The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Microbial community Phylogeny and function in relation to environment in the Norwegian Sea: A high-throughput community-sequencing-based assessment

Microbial community Phylogeny and function in relation to environment in the Norwegian Sea: A high-throughput community-sequencing-based assessment
Microbial community Phylogeny and function in relation to environment in the Norwegian Sea: A high-throughput community-sequencing-based assessment
Significant changes to seasonal ice cover, stratification, and warming are altering the oceanic boundaries between Polar and Atlantic Water masses. The resultant increased mixing and intrusion of Atlantic Waters into the Arctic region is resulting in novel competition between extant microbial communities that drive biogeochemical cycles and underpin the food-web in these
regions. However, it remains unclear how extant microbial communities will respond to these new opportunities and challenges. This work aims to provide an insight into how the bacterial and microbial eukaryotic communities present across a transect in the Norwegian Sea may be impacted by predicted future environmental change to the Arctic region through the use of Next Generation
Sequencing methodologies. It is revealed that the microbial communities of the region are being partitioned into distinct assemblages that correlate with gradients of temperature and salinity.
Analysis of the microbial communities from locations influenced by both Polar and Atlantic waters is used to indicate which components of the microbial communities will be selected for as these waters mix. The results of these analyses suggests the potential for the displacement of bacterial
communities found at locations determined to be highly influenced by Polar Water, by bacterial communities from locations found to be primarily influenced by Atlantic Waters. This response appears consistent for all abundance fractions and constituent taxonomic groups within the bacterial community.
Analysis of the eukaryotic community suggests a more complex response whereby abundant eukaryotic cold water associated species could dominate over temperate associated species, and different eukaryotic lineages display contrasting responses. Metatranscriptomes are generated for the
eukaryotic community to determine the functional differences between the regional communities. Partitioning was observed which matched the gradient of Polar Water influence implying the presence of distinct genetic profiles between regional communities.
Each station is observed to feature different profiles of gene expression for genes related to key ecosystem process including primary production, nutrient cycling, biogeochemical cycles, the carbon cycle and metabolic processes. However, despite some differences in the expression of functional profiles, functionality is found to be largely conserved across regional communities, suggesting increase Atlantic Water influence within the sampled region may not result in large perturbations to ecosystem functionality, despite potential changes to community composition.
This study has significant implications for the vulnerability of polar associated community assemblages, which may become displaced under predicted increases
of Atlantic mixing and warming within the Arctic region.
University of Southampton
Carter-Gates, Michael, James Alexander
d72c2af4-045b-4be0-855f-31356b3b33bd
Carter-Gates, Michael, James Alexander
d72c2af4-045b-4be0-855f-31356b3b33bd
Bibby, Thomas
e04ea079-dd90-4ead-9840-00882de27ebd

Carter-Gates, Michael, James Alexander (2019) Microbial community Phylogeny and function in relation to environment in the Norwegian Sea: A high-throughput community-sequencing-based assessment. University of Southampton, Doctoral Thesis, 301pp.

Record type: Thesis (Doctoral)

Abstract

Significant changes to seasonal ice cover, stratification, and warming are altering the oceanic boundaries between Polar and Atlantic Water masses. The resultant increased mixing and intrusion of Atlantic Waters into the Arctic region is resulting in novel competition between extant microbial communities that drive biogeochemical cycles and underpin the food-web in these
regions. However, it remains unclear how extant microbial communities will respond to these new opportunities and challenges. This work aims to provide an insight into how the bacterial and microbial eukaryotic communities present across a transect in the Norwegian Sea may be impacted by predicted future environmental change to the Arctic region through the use of Next Generation
Sequencing methodologies. It is revealed that the microbial communities of the region are being partitioned into distinct assemblages that correlate with gradients of temperature and salinity.
Analysis of the microbial communities from locations influenced by both Polar and Atlantic waters is used to indicate which components of the microbial communities will be selected for as these waters mix. The results of these analyses suggests the potential for the displacement of bacterial
communities found at locations determined to be highly influenced by Polar Water, by bacterial communities from locations found to be primarily influenced by Atlantic Waters. This response appears consistent for all abundance fractions and constituent taxonomic groups within the bacterial community.
Analysis of the eukaryotic community suggests a more complex response whereby abundant eukaryotic cold water associated species could dominate over temperate associated species, and different eukaryotic lineages display contrasting responses. Metatranscriptomes are generated for the
eukaryotic community to determine the functional differences between the regional communities. Partitioning was observed which matched the gradient of Polar Water influence implying the presence of distinct genetic profiles between regional communities.
Each station is observed to feature different profiles of gene expression for genes related to key ecosystem process including primary production, nutrient cycling, biogeochemical cycles, the carbon cycle and metabolic processes. However, despite some differences in the expression of functional profiles, functionality is found to be largely conserved across regional communities, suggesting increase Atlantic Water influence within the sampled region may not result in large perturbations to ecosystem functionality, despite potential changes to community composition.
This study has significant implications for the vulnerability of polar associated community assemblages, which may become displaced under predicted increases
of Atlantic mixing and warming within the Arctic region.

Text
Carter-Gates, Michael_PhD_Thesis_Dec_2019 - Author's Original
Restricted to Repository staff only until 1 January 2022.
Available under License University of Southampton Thesis Licence.

More information

Published date: 2019

Identifiers

Local EPrints ID: 436684
URI: http://eprints.soton.ac.uk/id/eprint/436684
PURE UUID: 94a0b5b1-c9a3-4039-9587-66a68848006a

Catalogue record

Date deposited: 20 Dec 2019 18:30
Last modified: 21 Nov 2021 19:13

Export record

Contributors

Author: Michael, James Alexander Carter-Gates
Thesis advisor: Thomas Bibby

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×