The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

In vitro effects of Bifidobacterium lactis-based synbiotics on human faecal bacteria

In vitro effects of Bifidobacterium lactis-based synbiotics on human faecal bacteria
In vitro effects of Bifidobacterium lactis-based synbiotics on human faecal bacteria
Synbiotic supplements contain pre- and probiotics and are used to modulate gut microbiota composition. This study aimed to investigate effects of two synbiotic mixtures on human faecal bacteria in vitro. Short chain fructooligosaccharides (FOS) (1% w/v) combined with either Bifidobacterium lactis Bb12 or Bifidobacterium lactis HN019 (106 colony-forming units (CFU)/mL)] were added to pH-controlled anaerobic batch cultures inoculated with human faeces. Maltodextrin (1% w/v), FOS (1% w/v) and the probiotic strains were also tested individually. Effects on bacteria, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were assessed over 48 h. With maltodextrin, FOS and the synbiotic mixtures, there was a significant increase in total bacteria and bifidobacteria numbers, compared to the negative control or probiotics alone. Increases in Atopobium cluster and Clostridium coccoides-Eubacterium rectale group occurred with FOS and maltodextrin, respectively. Additionally, maltodextrin, FOS and synbiotics resulted in a greater production of acetate and butyrate (SCFAs) compared to the negative control and probiotics alone, whereas concentrations of iso-valerate (BCFA) were lower with these treatments. In conclusion, synbiotic-induced in vitro bacterial changes and changes in SCFAs concentrations were not different from those observed with FOS alone. These data suggest that metabolic effects of these synbiotics are largely driven by the prebiotic component.
Batch culture system, Faecal microbiota, Maltodextrin, Prebiotic, Probiotic, Short-chain fatty acids
Henrique-Bana, Fernanda C.
11c07614-83fb-457b-8eb3-67767fff9549
Wang, Xuedan
ee500cff-fad1-4d52-bb76-ae9388b953ae
Costa, Giselle N.
40481b59-1ead-477a-8160-78cf2a8b66ae
Spinosa, Wilma A.
05947b5b-03c1-40c1-90f8-c49c88b237e6
Miglioranza, Lucia H. S.
d96fb62a-5b4f-4583-baef-d35afa336967
Scorletti, Eleonora
4e896544-2974-4f81-9696-1595d3c36814
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Byrne, Christopher D.
1370b997-cead-4229-83a7-53301ed2a43c
Gibson, Glenn R.
72eaa763-d677-4e72-8cd0-6afa0b7a5ab4
Henrique-Bana, Fernanda C.
11c07614-83fb-457b-8eb3-67767fff9549
Wang, Xuedan
ee500cff-fad1-4d52-bb76-ae9388b953ae
Costa, Giselle N.
40481b59-1ead-477a-8160-78cf2a8b66ae
Spinosa, Wilma A.
05947b5b-03c1-40c1-90f8-c49c88b237e6
Miglioranza, Lucia H. S.
d96fb62a-5b4f-4583-baef-d35afa336967
Scorletti, Eleonora
4e896544-2974-4f81-9696-1595d3c36814
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Byrne, Christopher D.
1370b997-cead-4229-83a7-53301ed2a43c
Gibson, Glenn R.
72eaa763-d677-4e72-8cd0-6afa0b7a5ab4

Henrique-Bana, Fernanda C., Wang, Xuedan, Costa, Giselle N., Spinosa, Wilma A., Miglioranza, Lucia H. S., Scorletti, Eleonora, Calder, Philip C., Byrne, Christopher D. and Gibson, Glenn R. (2020) In vitro effects of Bifidobacterium lactis-based synbiotics on human faecal bacteria. Food Research International, 128, [108776]. (doi:10.1016/j.foodres.2019.108776).

Record type: Article

Abstract

Synbiotic supplements contain pre- and probiotics and are used to modulate gut microbiota composition. This study aimed to investigate effects of two synbiotic mixtures on human faecal bacteria in vitro. Short chain fructooligosaccharides (FOS) (1% w/v) combined with either Bifidobacterium lactis Bb12 or Bifidobacterium lactis HN019 (106 colony-forming units (CFU)/mL)] were added to pH-controlled anaerobic batch cultures inoculated with human faeces. Maltodextrin (1% w/v), FOS (1% w/v) and the probiotic strains were also tested individually. Effects on bacteria, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were assessed over 48 h. With maltodextrin, FOS and the synbiotic mixtures, there was a significant increase in total bacteria and bifidobacteria numbers, compared to the negative control or probiotics alone. Increases in Atopobium cluster and Clostridium coccoides-Eubacterium rectale group occurred with FOS and maltodextrin, respectively. Additionally, maltodextrin, FOS and synbiotics resulted in a greater production of acetate and butyrate (SCFAs) compared to the negative control and probiotics alone, whereas concentrations of iso-valerate (BCFA) were lower with these treatments. In conclusion, synbiotic-induced in vitro bacterial changes and changes in SCFAs concentrations were not different from those observed with FOS alone. These data suggest that metabolic effects of these synbiotics are largely driven by the prebiotic component.

Text
Manuscript. Henrique-Bana et al. - Accepted Manuscript
Download (251kB)
Text
Figures and tables. Henrique-Bana et al. - Accepted Manuscript
Download (324kB)

More information

Accepted/In Press date: 28 October 2019
e-pub ahead of print date: 8 November 2019
Published date: February 2020
Keywords: Batch culture system, Faecal microbiota, Maltodextrin, Prebiotic, Probiotic, Short-chain fatty acids

Identifiers

Local EPrints ID: 436931
URI: http://eprints.soton.ac.uk/id/eprint/436931
PURE UUID: 980f88b6-552f-48ed-9ece-d73885b84471
ORCID for Philip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X
ORCID for Christopher D. Byrne: ORCID iD orcid.org/0000-0001-6322-7753

Catalogue record

Date deposited: 14 Jan 2020 17:30
Last modified: 26 Nov 2021 06:54

Export record

Altmetrics

Contributors

Author: Fernanda C. Henrique-Bana
Author: Xuedan Wang
Author: Giselle N. Costa
Author: Wilma A. Spinosa
Author: Lucia H. S. Miglioranza
Author: Eleonora Scorletti
Author: Glenn R. Gibson

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×