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Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria

Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria
Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria
A study to assess the diagnostic capabilities of three parasite lactate dehydrogenase (pan-pLDH) tests, Vistapan®, Carestart™ and Parabank®, was conducted in Uganda. An HRP2 test, Paracheck-Pf®, and a Giemsa-stained blood film were performed with the pLDH tests for outpatients with suspected malaria. In total, 460 subjects were recruited: 248 with positive blood films and 212 with negative blood films. Plasmodium falciparum was present in 95% of infections. Sensitivity above 90% was shown by two pLDH tests, Carestart (95.6%) and Vistapan (91.9%), and specificity above 90% by Parabank (94.3%) and Carestart (91.5%). Sensitivity decreased with low parasitaemia (χ2 trend, P <0.001); however, all tests achieved sensitivity >90% with parasitaemia ≥100/μl. All tests had good inter-reader reliability (κ > 0.95). Two weeks after diagnosis, 4–10% of pLDH tests were still positive compared with 69.7% of the HRP2 tests. All tests had similar ease of use. In conclusion, two pLDH tests performed well in diagnosing P. falciparum malaria, and all pLDH tests became negative after treatment more quickly than the HRP2. Therefore the rapid test of choice for use with artemisinin-combination therapies in this area would be one of these new pLDH tests.
0035-9203
25-31
Fogg, Carole
42057537-d443-462a-8944-c804252c973b
Twesigye, Roger
9912528d-5bc8-423d-85d0-0049029df10e
Batwala, Vincent
9b0222d2-3a6c-48a7-85bf-d7fbdc12bc77
Piola, Patrice
d5f4e60e-a212-4b81-9d3e-f0f6f3e5f440
Nabasumba, Carolyn
9c33c0ca-2079-4eef-aa0a-216dc1244184
Kiguli, James
19adf3bc-a43c-4033-94ed-bb15cf67705d
Mutebi, Frederick
c39086de-b826-48fe-8aa7-a38fcf2d0436
Hook, Christa
0a8200e5-67ef-4b27-8926-736d4bf44cc3
Guillerm, Martine
c0c3ce5e-14aa-4ea9-8c64-e5bbd195ac09
Moody, Anthony
9d1da79b-cd90-4336-a801-a2579011746f
Guthmann, Jean-Paul
ae239f74-b3b4-4f18-95fb-d5690d062d67
Fogg, Carole
42057537-d443-462a-8944-c804252c973b
Twesigye, Roger
9912528d-5bc8-423d-85d0-0049029df10e
Batwala, Vincent
9b0222d2-3a6c-48a7-85bf-d7fbdc12bc77
Piola, Patrice
d5f4e60e-a212-4b81-9d3e-f0f6f3e5f440
Nabasumba, Carolyn
9c33c0ca-2079-4eef-aa0a-216dc1244184
Kiguli, James
19adf3bc-a43c-4033-94ed-bb15cf67705d
Mutebi, Frederick
c39086de-b826-48fe-8aa7-a38fcf2d0436
Hook, Christa
0a8200e5-67ef-4b27-8926-736d4bf44cc3
Guillerm, Martine
c0c3ce5e-14aa-4ea9-8c64-e5bbd195ac09
Moody, Anthony
9d1da79b-cd90-4336-a801-a2579011746f
Guthmann, Jean-Paul
ae239f74-b3b4-4f18-95fb-d5690d062d67

Fogg, Carole, Twesigye, Roger, Batwala, Vincent, Piola, Patrice, Nabasumba, Carolyn, Kiguli, James, Mutebi, Frederick, Hook, Christa, Guillerm, Martine, Moody, Anthony and Guthmann, Jean-Paul (2008) Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria. Transactions of the Royal Society of Tropical Medicine and Hygiene, 102 (1), 25-31. (doi:10.1016/j.trstmh.2007.09.014).

Record type: Article

Abstract

A study to assess the diagnostic capabilities of three parasite lactate dehydrogenase (pan-pLDH) tests, Vistapan®, Carestart™ and Parabank®, was conducted in Uganda. An HRP2 test, Paracheck-Pf®, and a Giemsa-stained blood film were performed with the pLDH tests for outpatients with suspected malaria. In total, 460 subjects were recruited: 248 with positive blood films and 212 with negative blood films. Plasmodium falciparum was present in 95% of infections. Sensitivity above 90% was shown by two pLDH tests, Carestart (95.6%) and Vistapan (91.9%), and specificity above 90% by Parabank (94.3%) and Carestart (91.5%). Sensitivity decreased with low parasitaemia (χ2 trend, P <0.001); however, all tests achieved sensitivity >90% with parasitaemia ≥100/μl. All tests had good inter-reader reliability (κ > 0.95). Two weeks after diagnosis, 4–10% of pLDH tests were still positive compared with 69.7% of the HRP2 tests. All tests had similar ease of use. In conclusion, two pLDH tests performed well in diagnosing P. falciparum malaria, and all pLDH tests became negative after treatment more quickly than the HRP2. Therefore the rapid test of choice for use with artemisinin-combination therapies in this area would be one of these new pLDH tests.

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Accepted/In Press date: 25 September 2007
Published date: 1 January 2008

Identifiers

Local EPrints ID: 437004
URI: http://eprints.soton.ac.uk/id/eprint/437004
ISSN: 0035-9203
PURE UUID: bea78823-3b43-4b27-84a9-b2760c8d5242
ORCID for Carole Fogg: ORCID iD orcid.org/0000-0002-3000-6185

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Date deposited: 15 Jan 2020 17:30
Last modified: 27 Jan 2020 13:58

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Contributors

Author: Carole Fogg ORCID iD
Author: Roger Twesigye
Author: Vincent Batwala
Author: Patrice Piola
Author: Carolyn Nabasumba
Author: James Kiguli
Author: Frederick Mutebi
Author: Christa Hook
Author: Martine Guillerm
Author: Anthony Moody
Author: Jean-Paul Guthmann

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