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Avidity of serogroup A meningococcal IgG antibodies after immunization with different doses of a tetravalent A/C/Y/W135 polysaccharide vaccine

Avidity of serogroup A meningococcal IgG antibodies after immunization with different doses of a tetravalent A/C/Y/W135 polysaccharide vaccine
Avidity of serogroup A meningococcal IgG antibodies after immunization with different doses of a tetravalent A/C/Y/W135 polysaccharide vaccine
In the absence of an affordable conjugate meningococcal vaccine, mass vaccination campaigns with polysaccharide vaccines are the means to control meningitis epidemics in sub-Saharan Africa. Facing global vaccine shortage, the use of reduced doses, which have been shown to be protective by serum bactericidal activity, can save many lives. In this study, we investigated the antibody responses and avidity of IgG antibodies evoked against the serogroup A capsule of Neisseria meningitidis by different doses of an A/C/Y/W135 polysaccharide vaccine. Volunteers in Uganda were vaccinated with 1/10, 1/5 or a full dose (50 μg) and revaccinated with a full dose after 1 year. Specific IgG geometric mean concentrations and geometric mean avidity indices (GMAI) were determined by a modified enzyme-linked immunosorbent assay (ELISA) using thiocyanate as a chaotropic agent. After vaccination with 1/10 or 1/5 doses, the GMAI increased from 1 month to 1 year. One year following the initial dose, the GMAI levels were higher in the arm receiving reduced doses than for the arm receiving a full dose. Following the second full dose, avidity indices equalized at approximately the same level in the three arms. Although there are practical challenges to the use of reduced doses in the field, our findings suggest that reduced doses of polysaccharide vaccine are able to elicit antibodies of as good avidity against serogroup A polysaccharide as a full dose.
0300-9475
87-94
Barnes, G. K.
2a2219b1-94e7-46cb-b51b-b7622277f4bc
Naess, Lisbeth M.
679f154b-8aca-4393-932f-fd344c0343c0
Rosenqvist, Einar
1297a80b-f2bd-41db-af98-d0eb2d21fa43
Guerin, Philippe J.
78394525-7c10-4fd9-b2cd-4840aaa2dcb2
Caugant, Dominique A.
836e66dc-88e0-4711-80f5-a48b5f9756b3
Fogg, Carole
42057537-d443-462a-8944-c804252c973b
Barnes, G. K.
2a2219b1-94e7-46cb-b51b-b7622277f4bc
Naess, Lisbeth M.
679f154b-8aca-4393-932f-fd344c0343c0
Rosenqvist, Einar
1297a80b-f2bd-41db-af98-d0eb2d21fa43
Guerin, Philippe J.
78394525-7c10-4fd9-b2cd-4840aaa2dcb2
Caugant, Dominique A.
836e66dc-88e0-4711-80f5-a48b5f9756b3
Fogg, Carole
42057537-d443-462a-8944-c804252c973b

Barnes, G. K., Naess, Lisbeth M., Rosenqvist, Einar, Guerin, Philippe J., Caugant, Dominique A. and Fogg, Carole (2011) Avidity of serogroup A meningococcal IgG antibodies after immunization with different doses of a tetravalent A/C/Y/W135 polysaccharide vaccine. Scandinavian Journal of Immunology, 74 (1), 87-94. (doi:10.1111/j.1365-3083.2011.02535.x).

Record type: Article

Abstract

In the absence of an affordable conjugate meningococcal vaccine, mass vaccination campaigns with polysaccharide vaccines are the means to control meningitis epidemics in sub-Saharan Africa. Facing global vaccine shortage, the use of reduced doses, which have been shown to be protective by serum bactericidal activity, can save many lives. In this study, we investigated the antibody responses and avidity of IgG antibodies evoked against the serogroup A capsule of Neisseria meningitidis by different doses of an A/C/Y/W135 polysaccharide vaccine. Volunteers in Uganda were vaccinated with 1/10, 1/5 or a full dose (50 μg) and revaccinated with a full dose after 1 year. Specific IgG geometric mean concentrations and geometric mean avidity indices (GMAI) were determined by a modified enzyme-linked immunosorbent assay (ELISA) using thiocyanate as a chaotropic agent. After vaccination with 1/10 or 1/5 doses, the GMAI increased from 1 month to 1 year. One year following the initial dose, the GMAI levels were higher in the arm receiving reduced doses than for the arm receiving a full dose. Following the second full dose, avidity indices equalized at approximately the same level in the three arms. Although there are practical challenges to the use of reduced doses in the field, our findings suggest that reduced doses of polysaccharide vaccine are able to elicit antibodies of as good avidity against serogroup A polysaccharide as a full dose.

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e-pub ahead of print date: 17 February 2011
Published date: 1 July 2011

Identifiers

Local EPrints ID: 437020
URI: http://eprints.soton.ac.uk/id/eprint/437020
ISSN: 0300-9475
PURE UUID: 887c61b4-69ef-47bd-bfd4-cac59b15d4c0
ORCID for Carole Fogg: ORCID iD orcid.org/0000-0002-3000-6185

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Date deposited: 15 Jan 2020 17:31
Last modified: 07 Oct 2020 02:24

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Contributors

Author: G. K. Barnes
Author: Lisbeth M. Naess
Author: Einar Rosenqvist
Author: Philippe J. Guerin
Author: Dominique A. Caugant
Author: Carole Fogg ORCID iD

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