Investigating a suitable model for the study of vitamin D mediated regulation of human placental gene expression
Investigating a suitable model for the study of vitamin D mediated regulation of human placental gene expression
Transfer and metabolism of vitamin D across the human placenta is required for fetal development. However, these fundamental mechanisms are not well understood and model systems are required to help understand them. The BeWo choriocarcinoma cell line is derived from extravillous trophoblast but is used as a model for villous syncytiotrophoblast and the placental barrier. Questions have been raised about the suitability of the BeWo cell line as a model for villous trophoblast. This study compares the expression of amino acid transporters and vitamin D related genes in human term placenta with the BeWo and human embryonic kidney (HEK)293 cell lines. HEK293 cells, as transporting epithelium may be more similar to placenta. Gene expression in term placenta was much more similar to HEK293 than BeWo. This study provides further evidence that the BeWo cell line is not an appropriate model for villous trophoblast and a model that more closely represents the human placenta is now required to investigate the effects of vitamin D on the placenta ex-vivo.
Amino acid transporters, Placenta, Vitamin D
Simner, Claire
5390a8d9-733e-4336-9017-15057d049b2f
Ashley, Brogan
9124861e-115b-422b-adda-6582a369eece
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Harvey, Nicholas
ce487fb4-d360-4aac-9d17-9466d6cba145
Lewis, Rohan
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Cleal, Jane
18cfd2c1-bd86-4a13-b38f-c321af56da66
May 2020
Simner, Claire
5390a8d9-733e-4336-9017-15057d049b2f
Ashley, Brogan
9124861e-115b-422b-adda-6582a369eece
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Harvey, Nicholas
ce487fb4-d360-4aac-9d17-9466d6cba145
Lewis, Rohan
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Cleal, Jane
18cfd2c1-bd86-4a13-b38f-c321af56da66
Simner, Claire, Ashley, Brogan, Cooper, Cyrus, Harvey, Nicholas, Lewis, Rohan and Cleal, Jane
(2020)
Investigating a suitable model for the study of vitamin D mediated regulation of human placental gene expression.
The Journal of Steroid Biochemistry and Molecular Biology, 199, [105576].
(doi:10.1016/j.jsbmb.2019.105576).
Abstract
Transfer and metabolism of vitamin D across the human placenta is required for fetal development. However, these fundamental mechanisms are not well understood and model systems are required to help understand them. The BeWo choriocarcinoma cell line is derived from extravillous trophoblast but is used as a model for villous syncytiotrophoblast and the placental barrier. Questions have been raised about the suitability of the BeWo cell line as a model for villous trophoblast. This study compares the expression of amino acid transporters and vitamin D related genes in human term placenta with the BeWo and human embryonic kidney (HEK)293 cell lines. HEK293 cells, as transporting epithelium may be more similar to placenta. Gene expression in term placenta was much more similar to HEK293 than BeWo. This study provides further evidence that the BeWo cell line is not an appropriate model for villous trophoblast and a model that more closely represents the human placenta is now required to investigate the effects of vitamin D on the placenta ex-vivo.
Text
Clean Revision Simner et al 031219
- Accepted Manuscript
More information
Accepted/In Press date: 31 December 2019
e-pub ahead of print date: 2 January 2020
Published date: May 2020
Additional Information:
Funding Information:
This research was supported by the Gerald Kerkut Charitable Trust, UK .
Publisher Copyright:
© 2020 Elsevier Ltd
Keywords:
Amino acid transporters, Placenta, Vitamin D
Identifiers
Local EPrints ID: 437159
URI: http://eprints.soton.ac.uk/id/eprint/437159
ISSN: 0960-0760
PURE UUID: 9ebd4421-de6e-4c66-a7c9-cca6948d9112
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Date deposited: 20 Jan 2020 17:31
Last modified: 18 Mar 2024 05:04
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Author:
Claire Simner
Author:
Brogan Ashley
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