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A porphyrin-DNA chiroptical molecular ruler with base pair resolution

A porphyrin-DNA chiroptical molecular ruler with base pair resolution
A porphyrin-DNA chiroptical molecular ruler with base pair resolution
DNA-based molecular rulers enable scientists to determine important parameters across biology, from the measurement of protein binding interactions, to the study of membrane dynamics in cells. However existing rulers can suffer from poor nanometre resolution due to the flexible nature of linkers used to tether to the DNA framework. We aimed to overcome this problem using zinc and free-base porphyrin chromophores attached via short and rigid acetylene linkers. This connectivity enables the distance and angle between the porphyrins to be fine-tuned along the DNA scaffold. The porphyrins undergo favourable energy transfer and chiral exciton coupling interactions to act as highly sensitive molecular ruler probes. To validate the system, we monitored the detection of small changes in DNA structure upon intercalation of ethidium bromide. CD spectroscopy showed the porphyrins undergo highly sensitive changes in excitation coupling to facilitate base pair resolution of the novel system.
DNA, porphyrin, FRET, CD spectroscopy, ethidium bromide intercalation, exciton coupling
2296-2646
Burns, Jonathan R.
648a50f5-bcca-4989-8de3-60cc582b7ed9
Wood, James W.
2a643a0a-6f82-4a2c-9c3b-326d8bff06b5
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d
Burns, Jonathan R.
648a50f5-bcca-4989-8de3-60cc582b7ed9
Wood, James W.
2a643a0a-6f82-4a2c-9c3b-326d8bff06b5
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d

Burns, Jonathan R., Wood, James W. and Stulz, Eugen (2020) A porphyrin-DNA chiroptical molecular ruler with base pair resolution. Frontiers in Chemistry, 8, [113]. (doi:10.3389/fchem.2020.00113).

Record type: Article

Abstract

DNA-based molecular rulers enable scientists to determine important parameters across biology, from the measurement of protein binding interactions, to the study of membrane dynamics in cells. However existing rulers can suffer from poor nanometre resolution due to the flexible nature of linkers used to tether to the DNA framework. We aimed to overcome this problem using zinc and free-base porphyrin chromophores attached via short and rigid acetylene linkers. This connectivity enables the distance and angle between the porphyrins to be fine-tuned along the DNA scaffold. The porphyrins undergo favourable energy transfer and chiral exciton coupling interactions to act as highly sensitive molecular ruler probes. To validate the system, we monitored the detection of small changes in DNA structure upon intercalation of ethidium bromide. CD spectroscopy showed the porphyrins undergo highly sensitive changes in excitation coupling to facilitate base pair resolution of the novel system.

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Accepted/In Press date: 6 February 2020
Published date: 26 February 2020
Keywords: DNA, porphyrin, FRET, CD spectroscopy, ethidium bromide intercalation, exciton coupling

Identifiers

Local EPrints ID: 437895
URI: http://eprints.soton.ac.uk/id/eprint/437895
ISSN: 2296-2646
PURE UUID: c551d222-5963-454c-9a5a-1ff92ad08f58
ORCID for Eugen Stulz: ORCID iD orcid.org/0000-0002-5302-2276

Catalogue record

Date deposited: 21 Feb 2020 17:31
Last modified: 26 Nov 2021 06:15

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Contributors

Author: Jonathan R. Burns
Author: James W. Wood
Author: Eugen Stulz ORCID iD

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