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Comparative anti-inflammatory effects of plant- and marine-derived omega-3 fatty acids explored in an endothelial cell line

Comparative anti-inflammatory effects of plant- and marine-derived omega-3 fatty acids explored in an endothelial cell line
Comparative anti-inflammatory effects of plant- and marine-derived omega-3 fatty acids explored in an endothelial cell line
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) lower risk of cardiovascular disease. The primary source of EPA and DHA is fatty fish. Plant-derived alpha linolenic acid (ALA) and stearidonic acid (SDA) could provide sustainable land-based alternatives, but their functionality is underexplored. Omega-3 fatty acids (n-3 FAs) may influence atherogenic processes through changing endothelial cell (EC) function and lowering inflammation. This study compared effects of marine- and plant-derived n-3 FAs on EC inflammatory responses. EA.hy926 cells were exposed to ALA, SDA, EPA or DHA prior to stimulation with tumor necrosis factor (TNF)-α. All FAs were shown to be incorporated into ECs in a dose-dependent manner. SDA (50 μM) decreased both production and cell-surface expression of intercellular adhesion molecule (ICAM)-1; however EPA and DHA resulted in greater reduction of ICAM-1 production and expression. EPA and DHA also significantly lowered production of monocyte chemoattractant protein 1, interleukin (IL)-6 and IL-8. ALA, SDA and DHA (50 μM) all reduced adhesion of THP-1 monocytes to EA.hy926 cells. DHA significantly decreased nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)p105 gene expression and phosphorylated NFκBp65 protein. Both EPA and DHA (50 μM) significantly decreased cyclooxygenase (COX)-2 protein. Thus, both marine-derived n-3 FAs, particularly DHA, had potent anti-inflammatory effects in this EC model. Of the plant-derived n-3 FAs, SDA showed the greatest inhibition of inflammation. Although neither ALA nor SDA reproduced the anti-inflammatory effects of EPA and DHA in this model, there is some potential for SDA to be a sustainable anti-inflammatory alternative to the marine n-3 FAs.
Atherosclerosis, Cytokine, Endothelial cell, Inflammation, Omega-3, Plant and marine-derived fatty acids
1388-1981
Baker, Ella
7cd5b762-d7d7-4584-b9a7-dba555085440
Valenzuela, Carina, Alejandra
1a12a9b9-6504-4392-90c5-246644b0ad5c
de Souza, Camila
df8676e9-b890-492f-8725-bcfe652277c3
Yaqoob, Parveen
26b58c23-1716-4dc6-adfe-45b8bc846d76
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Baker, Ella
7cd5b762-d7d7-4584-b9a7-dba555085440
Valenzuela, Carina, Alejandra
1a12a9b9-6504-4392-90c5-246644b0ad5c
de Souza, Camila
df8676e9-b890-492f-8725-bcfe652277c3
Yaqoob, Parveen
26b58c23-1716-4dc6-adfe-45b8bc846d76
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6

Baker, Ella, Valenzuela, Carina, Alejandra, de Souza, Camila, Yaqoob, Parveen, Miles, Elizabeth and Calder, Philip (2020) Comparative anti-inflammatory effects of plant- and marine-derived omega-3 fatty acids explored in an endothelial cell line. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1865 (6), [158662]. (doi:10.1016/j.bbalip.2020.158662).

Record type: Article

Abstract

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) lower risk of cardiovascular disease. The primary source of EPA and DHA is fatty fish. Plant-derived alpha linolenic acid (ALA) and stearidonic acid (SDA) could provide sustainable land-based alternatives, but their functionality is underexplored. Omega-3 fatty acids (n-3 FAs) may influence atherogenic processes through changing endothelial cell (EC) function and lowering inflammation. This study compared effects of marine- and plant-derived n-3 FAs on EC inflammatory responses. EA.hy926 cells were exposed to ALA, SDA, EPA or DHA prior to stimulation with tumor necrosis factor (TNF)-α. All FAs were shown to be incorporated into ECs in a dose-dependent manner. SDA (50 μM) decreased both production and cell-surface expression of intercellular adhesion molecule (ICAM)-1; however EPA and DHA resulted in greater reduction of ICAM-1 production and expression. EPA and DHA also significantly lowered production of monocyte chemoattractant protein 1, interleukin (IL)-6 and IL-8. ALA, SDA and DHA (50 μM) all reduced adhesion of THP-1 monocytes to EA.hy926 cells. DHA significantly decreased nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)p105 gene expression and phosphorylated NFκBp65 protein. Both EPA and DHA (50 μM) significantly decreased cyclooxygenase (COX)-2 protein. Thus, both marine-derived n-3 FAs, particularly DHA, had potent anti-inflammatory effects in this EC model. Of the plant-derived n-3 FAs, SDA showed the greatest inhibition of inflammation. Although neither ALA nor SDA reproduced the anti-inflammatory effects of EPA and DHA in this model, there is some potential for SDA to be a sustainable anti-inflammatory alternative to the marine n-3 FAs.

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More information

Accepted/In Press date: 8 February 2020
e-pub ahead of print date: 11 February 2020
Published date: June 2020
Keywords: Atherosclerosis, Cytokine, Endothelial cell, Inflammation, Omega-3, Plant and marine-derived fatty acids

Identifiers

Local EPrints ID: 437986
URI: http://eprints.soton.ac.uk/id/eprint/437986
ISSN: 1388-1981
PURE UUID: cb27b31d-9067-46f2-9532-84e2e84524b6
ORCID for Elizabeth Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 25 Feb 2020 17:31
Last modified: 22 Nov 2021 08:02

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Contributors

Author: Ella Baker
Author: Carina, Alejandra Valenzuela
Author: Camila de Souza
Author: Parveen Yaqoob
Author: Elizabeth Miles ORCID iD
Author: Philip Calder ORCID iD

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