High-speed single-cell dielectric spectroscopy
High-speed single-cell dielectric spectroscopy
Single-cell impedance cytometry is a label-free analysis technique that is now widely used to measure the electrical properties of a cell and to differentiate different subpopulations. Current techniques are limited to measuring the impedance of a single cell at one or two simultaneous frequencies. Also, there are no methods that extrapolate the intrinsic electrical properties of single cells. We demonstrate a new approach that uses multifrequency impedance measurements to determine the complete intrinsic electrical properties of thousands of single cells at high throughput. The applicability of the method is demonstrated by measuring the properties of red blood cells and red cell ghosts, deriving the unique values of conductivity and permittivity of the membrane and cytoplasm for each individual cell.
cytometry, dielectric spectroscopy, impedance analysis, microfluidics, single-cell analysis
423-430
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
28 February 2020
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Abstract
Single-cell impedance cytometry is a label-free analysis technique that is now widely used to measure the electrical properties of a cell and to differentiate different subpopulations. Current techniques are limited to measuring the impedance of a single cell at one or two simultaneous frequencies. Also, there are no methods that extrapolate the intrinsic electrical properties of single cells. We demonstrate a new approach that uses multifrequency impedance measurements to determine the complete intrinsic electrical properties of thousands of single cells at high throughput. The applicability of the method is demonstrated by measuring the properties of red blood cells and red cell ghosts, deriving the unique values of conductivity and permittivity of the membrane and cytoplasm for each individual cell.
Text
High-Speed Single-Cell Dielectric
- Accepted Manuscript
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e-pub ahead of print date: 4 February 2020
Published date: 28 February 2020
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Publisher Copyright:
Copyright © 2020 American Chemical Society.
Keywords:
cytometry, dielectric spectroscopy, impedance analysis, microfluidics, single-cell analysis
Identifiers
Local EPrints ID: 437989
URI: http://eprints.soton.ac.uk/id/eprint/437989
ISSN: 2379-3694
PURE UUID: 1d62f6d3-880f-413d-a2f2-168478278173
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Date deposited: 25 Feb 2020 17:31
Last modified: 17 Mar 2024 05:20
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Author:
Daniel Spencer
Author:
Hywel Morgan
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