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High-speed single-cell dielectric spectroscopy

High-speed single-cell dielectric spectroscopy
High-speed single-cell dielectric spectroscopy
Single-cell impedance cytometry is a label-free analysis technique that is now widely used to measure the electrical properties of a cell and to differentiate different subpopulations. Current techniques are limited to measuring the impedance of a single cell at one or two simultaneous frequencies. Also, there are no methods that extrapolate the intrinsic electrical properties of single cells. We demonstrate a new approach that uses multifrequency impedance measurements to determine the complete intrinsic electrical properties of thousands of single cells at high throughput. The applicability of the method is demonstrated by measuring the properties of red blood cells and red cell ghosts, deriving the unique values of conductivity and permittivity of the membrane and cytoplasm for each individual cell.
cytometry, dielectric spectroscopy, impedance analysis, microfluidics, single-cell analysis
2379-3694
423-430
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Spencer, Daniel and Morgan, Hywel (2020) High-speed single-cell dielectric spectroscopy. ACS Sensors, 5 (2), 423-430. (doi:10.1021/acssensors.9b02119).

Record type: Article

Abstract

Single-cell impedance cytometry is a label-free analysis technique that is now widely used to measure the electrical properties of a cell and to differentiate different subpopulations. Current techniques are limited to measuring the impedance of a single cell at one or two simultaneous frequencies. Also, there are no methods that extrapolate the intrinsic electrical properties of single cells. We demonstrate a new approach that uses multifrequency impedance measurements to determine the complete intrinsic electrical properties of thousands of single cells at high throughput. The applicability of the method is demonstrated by measuring the properties of red blood cells and red cell ghosts, deriving the unique values of conductivity and permittivity of the membrane and cytoplasm for each individual cell.

Text
High-Speed Single-Cell Dielectric - Accepted Manuscript
Restricted to Repository staff only until 4 February 2021.
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More information

e-pub ahead of print date: 4 February 2020
Keywords: cytometry, dielectric spectroscopy, impedance analysis, microfluidics, single-cell analysis

Identifiers

Local EPrints ID: 437989
URI: http://eprints.soton.ac.uk/id/eprint/437989
ISSN: 2379-3694
PURE UUID: 1d62f6d3-880f-413d-a2f2-168478278173
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 25 Feb 2020 17:31
Last modified: 08 Oct 2020 16:51

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Contributors

Author: Daniel Spencer
Author: Hywel Morgan ORCID iD

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