READ ME File For 'Dataset for: Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering' Dataset DOI: https://doi.org/10.5258/SOTON/D1173 ReadMe Author: Dr Jonathan Butement, University of Southampton Feb 2020 This dataset supports the publication: Jonathan T. Butement, Paul M. Holloway, Joshua A. Welsh, Judith A. Holloway, Nicola A. Englyst, Peter Horak, Jonathan West and James S. Wilkinson (2019) Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering RsC Lab on a chip 2020 Filename: Description: Flow_cytometry_matlab_scripts.zip Matlab scripts for analysis of flow cytometry data Paper_figures.zip .tif files for paper and SI figures. Fig_1c_MMI_imaging_length_model_data.zip .txt file with MMI electric field strength from BPM model and matlab script for comparison to analytical model fig_4_MMI beam imaging.zip .tif files of fluorescently imaged MMI beam Fig_5a_dean_focussing_imaging.zip Fluorescence microscopy images used to calculate dean focussing effectiveness Fig_5b_2mps_confocal_flow_focussing.avi video summary of confocal data for figure 5b ******************nm_pulse_data.zip .mat data files with pmt voltage pulses recorded for size standard samples generated from raw tpc5 files using 'EVC_trace_analysis_2018_multiblock__multifile_v05_p1000.m'shows pulse shapes for figs 6a and SI fig 1 DPLC200nm_pulse_data.zip .mat DPLC liposome data files with pmt voltage pulses recorded for size standard samples generated from raw tpc5 files using 'EVC_trace_analysis_2018_multiblock__multifile_v05_p1000.m for pulse shapes for SI fig 4a Relative_scattering_distribution_data.zip .mat data files of relative scattering distribution saved from running 'EVC_combi_v11_p400_3D_750mms_t0_7m_time_corr.m' matlab script for fig 7 and SI-fig 5-6 Geographic location of data collection: University of Southampton, U.K. Date of data collection 2016-07-01 to 2019-12-01 Related projects: European Union’s Seventh Framework Programme (FP7/2007–2013) ERC grant agreement no. 291216 ‘Wideband Integrated Photonics for Accessible Biomedical Diagnostics’ Medical Research Council for a Discovery Grant (JTB, MC_PC_15078) Dataset available under a CC BY-NC 4.0 licence Publisher: University of Southampton, U.K. Date: Feb 2020