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A comparative synthesis of transcriptomic analyses reveals major differences between WSSV-susceptible Litopenaeus vannamei and WSSV-refractory Macrobrachium rosenbergii

A comparative synthesis of transcriptomic analyses reveals major differences between WSSV-susceptible Litopenaeus vannamei and WSSV-refractory Macrobrachium rosenbergii
A comparative synthesis of transcriptomic analyses reveals major differences between WSSV-susceptible Litopenaeus vannamei and WSSV-refractory Macrobrachium rosenbergii
Since the 1990s White Spot Syndrome Virus (WSSV) has severely affected shrimp aquaculture worldwide causing a global pandemic of White Spot Disease (WSD) in penaeid culture. However, not all decapod species that can be infected by WSSV show the same susceptibility to the virus, thus raising interesting questions regarding the potential genetic traits that might confer resistance to WSSV. In order to shed light into the genetic markers of WSSV resistance, we employed a dual approach: i) we initially analysed the transcriptomes derived from the hepatopancreas of two species, the susceptible white shrimp Litopenaeus vannamei and the refractory fresh water prawn Macrobrachium rosenbergii, both infected with WSSV. We found a large number of differentially expressed genes (DEGs) belonging to the immune system (mostly anti-microbial peptides and haemolymph clotting components) that were generally up-regulated in M. rosenbergii and down-regulated in L. vannamei. Further, in both species we identified many up-regulated DEGs that were related to metabolism (suggesting a metabolic shift during the infection) and, interestingly, in L. vannamei only, we found several DEGs that were related to moult and suggested an inhibition of the moult cycle in this species following WSSV infection. ii) we then identified a limited number of genetic markers putatively linked with WSD tolerance by employing an ecological genomics approach in which we compared published reports with our own RNA-seq datasets for different decapod species infected with WSSV. Using this second comparative approach, we found nine candidate genes which are consistently down-regulated in susceptible species and up-regulated in refractory species and which have a role in immune response. Together our data offer novel insights into gene expression differences that can be found in susceptible and refractory decapod species infected with WSSV and provide a valuable resource towards our understanding of the potential genetic basis of tolerance to WSSV.
Refractory Macrobrachium rosenbergii, Susceptible Litopenaeus vannamei, White spot syndrome virus
0145-305X
Peruzza, L.
97dfdd24-f9e8-4d76-aee5-67bdd9b92541
Thamizhvanan, S.
120cdeaa-0213-47ac-bfc5-321aae723ea9
Vimal, S.
7e4fa71a-fa89-4f08-ac09-0c09b2a1909f
Vinaya Kumar, K.
6dcd97cd-cf67-46ef-8469-e4d5b3d9c84a
Shekhar, M.s.
7cc1c236-cf3e-42d5-a131-792ddd0af0c1
Smith, V.j.
59122748-f7c5-4d7e-ad0a-c522df9f8e84
Hauton, C.
7706f6ba-4497-42b2-8c6d-00df81676331
Vijayan, K.k.
0b05a8b9-f6c8-4c37-8b34-6012fc746830
Sahul Hameed, A.s.
8af1f9f5-408e-4ef6-80be-8c446ffc6634
Peruzza, L.
97dfdd24-f9e8-4d76-aee5-67bdd9b92541
Thamizhvanan, S.
120cdeaa-0213-47ac-bfc5-321aae723ea9
Vimal, S.
7e4fa71a-fa89-4f08-ac09-0c09b2a1909f
Vinaya Kumar, K.
6dcd97cd-cf67-46ef-8469-e4d5b3d9c84a
Shekhar, M.s.
7cc1c236-cf3e-42d5-a131-792ddd0af0c1
Smith, V.j.
59122748-f7c5-4d7e-ad0a-c522df9f8e84
Hauton, C.
7706f6ba-4497-42b2-8c6d-00df81676331
Vijayan, K.k.
0b05a8b9-f6c8-4c37-8b34-6012fc746830
Sahul Hameed, A.s.
8af1f9f5-408e-4ef6-80be-8c446ffc6634

Peruzza, L., Thamizhvanan, S., Vimal, S., Vinaya Kumar, K., Shekhar, M.s., Smith, V.j., Hauton, C., Vijayan, K.k. and Sahul Hameed, A.s. (2020) A comparative synthesis of transcriptomic analyses reveals major differences between WSSV-susceptible Litopenaeus vannamei and WSSV-refractory Macrobrachium rosenbergii. Developmental & Comparative Immunology, 104, [103564]. (doi:10.1016/j.dci.2019.103564).

Record type: Article

Abstract

Since the 1990s White Spot Syndrome Virus (WSSV) has severely affected shrimp aquaculture worldwide causing a global pandemic of White Spot Disease (WSD) in penaeid culture. However, not all decapod species that can be infected by WSSV show the same susceptibility to the virus, thus raising interesting questions regarding the potential genetic traits that might confer resistance to WSSV. In order to shed light into the genetic markers of WSSV resistance, we employed a dual approach: i) we initially analysed the transcriptomes derived from the hepatopancreas of two species, the susceptible white shrimp Litopenaeus vannamei and the refractory fresh water prawn Macrobrachium rosenbergii, both infected with WSSV. We found a large number of differentially expressed genes (DEGs) belonging to the immune system (mostly anti-microbial peptides and haemolymph clotting components) that were generally up-regulated in M. rosenbergii and down-regulated in L. vannamei. Further, in both species we identified many up-regulated DEGs that were related to metabolism (suggesting a metabolic shift during the infection) and, interestingly, in L. vannamei only, we found several DEGs that were related to moult and suggested an inhibition of the moult cycle in this species following WSSV infection. ii) we then identified a limited number of genetic markers putatively linked with WSD tolerance by employing an ecological genomics approach in which we compared published reports with our own RNA-seq datasets for different decapod species infected with WSSV. Using this second comparative approach, we found nine candidate genes which are consistently down-regulated in susceptible species and up-regulated in refractory species and which have a role in immune response. Together our data offer novel insights into gene expression differences that can be found in susceptible and refractory decapod species infected with WSSV and provide a valuable resource towards our understanding of the potential genetic basis of tolerance to WSSV.

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More information

Accepted/In Press date: 30 November 2019
e-pub ahead of print date: 6 December 2019
Published date: 1 March 2020
Keywords: Refractory Macrobrachium rosenbergii, Susceptible Litopenaeus vannamei, White spot syndrome virus

Identifiers

Local EPrints ID: 438371
URI: http://eprints.soton.ac.uk/id/eprint/438371
ISSN: 0145-305X
PURE UUID: 4446bbe6-2ec3-4514-aee3-df118d2339da
ORCID for C. Hauton: ORCID iD orcid.org/0000-0002-2313-4226

Catalogue record

Date deposited: 06 Mar 2020 17:34
Last modified: 26 Nov 2021 02:38

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Contributors

Author: L. Peruzza
Author: S. Thamizhvanan
Author: S. Vimal
Author: K. Vinaya Kumar
Author: M.s. Shekhar
Author: V.j. Smith
Author: C. Hauton ORCID iD
Author: K.k. Vijayan
Author: A.s. Sahul Hameed

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