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Creating supported plasma membrane bilayers using acoustic pressure

Creating supported plasma membrane bilayers using acoustic pressure
Creating supported plasma membrane bilayers using acoustic pressure
Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics.
Acoustic pressure, GPMVs, Plasma membrane bilayers, Plasma membrane vesicles, Supported bilayers
2077-0375
Sezgin, Erdinc
7b0b5507-2421-46f8-8ef0-04a7bc510348
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Levental, Ilya
b8f94f05-786e-4c19-b14a-a982a458d981
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
Eggeling, Christian
8003f376-30f6-49b4-bb50-bab8545b5ffd
Sezgin, Erdinc
7b0b5507-2421-46f8-8ef0-04a7bc510348
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Levental, Ilya
b8f94f05-786e-4c19-b14a-a982a458d981
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
Eggeling, Christian
8003f376-30f6-49b4-bb50-bab8545b5ffd

Sezgin, Erdinc, Carugo, Dario, Levental, Ilya, Stride, Eleanor and Eggeling, Christian (2020) Creating supported plasma membrane bilayers using acoustic pressure. Membranes, 10 (30), [30]. (doi:10.3390/membranes10020030).

Record type: Article

Abstract

Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics.

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More information

Accepted/In Press date: 14 February 2020
Published date: 18 February 2020
Keywords: Acoustic pressure, GPMVs, Plasma membrane bilayers, Plasma membrane vesicles, Supported bilayers

Identifiers

Local EPrints ID: 438380
URI: http://eprints.soton.ac.uk/id/eprint/438380
ISSN: 2077-0375
PURE UUID: f9539bd0-0f46-4c9f-95cf-1cab50ed8e75

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Date deposited: 09 Mar 2020 17:30
Last modified: 25 Nov 2021 21:30

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Contributors

Author: Erdinc Sezgin
Author: Dario Carugo
Author: Ilya Levental
Author: Eleanor Stride
Author: Christian Eggeling

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