Creating supported plasma membrane bilayers using acoustic pressure
Creating supported plasma membrane bilayers using acoustic pressure
Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics.
Acoustic pressure, GPMVs, Plasma membrane bilayers, Plasma membrane vesicles, Supported bilayers
Sezgin, Erdinc
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Carugo, Dario
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Levental, Ilya
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Stride, Eleanor
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Eggeling, Christian
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18 February 2020
Sezgin, Erdinc
7b0b5507-2421-46f8-8ef0-04a7bc510348
Carugo, Dario
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Levental, Ilya
b8f94f05-786e-4c19-b14a-a982a458d981
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
Eggeling, Christian
8003f376-30f6-49b4-bb50-bab8545b5ffd
Sezgin, Erdinc, Carugo, Dario, Levental, Ilya, Stride, Eleanor and Eggeling, Christian
(2020)
Creating supported plasma membrane bilayers using acoustic pressure.
Membranes, 10 (2), [30].
(doi:10.3390/membranes10020030).
Abstract
Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics.
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membranes-10-00030 (1)
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Accepted/In Press date: 14 February 2020
Published date: 18 February 2020
Additional Information:
Funding Information:
Funding: We acknowledge funding by the Wolfson Foundation, the Medical Research Council (MRC, grant number MC_UU_12010/unit programmes G0902418 and MC_UU_12025), MRC/BBSRC/EPSRC (grant number MR/K01577X/1), the Wellcome Trust (grant ref 104924/14/Z/14), the Deutsche Forschungsgemeinschaft (Research unit 1905 “Structure and function of the peroxisomal translocon”), Oxford—internal funds (John Fell Fund and EPA Cephalosporin Fund) and Wellcome Institutional Strategic Support Fund (ISSF). ES is funded by the Newton-Katip Celebi Institutional Links grant (352333122).
Funding Information:
Acknowledgments: We thank Umesh Sai Jonnalagadda and Elisabetta Bottaro for their effort on the acoustic devices, and James Fisk and David Salisbury for fabricating the acoustofluidic device’s components. We thank the Wolfson Imaging Centre Oxford and the Micron Advanced Bioimaging Unit (Wellcome Trust Strategic Award 091911) for providing microscope facility and financial support.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords:
Acoustic pressure, GPMVs, Plasma membrane bilayers, Plasma membrane vesicles, Supported bilayers
Identifiers
Local EPrints ID: 438380
URI: http://eprints.soton.ac.uk/id/eprint/438380
ISSN: 2077-0375
PURE UUID: f9539bd0-0f46-4c9f-95cf-1cab50ed8e75
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Date deposited: 09 Mar 2020 17:30
Last modified: 16 Mar 2024 06:52
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Contributors
Author:
Erdinc Sezgin
Author:
Ilya Levental
Author:
Eleanor Stride
Author:
Christian Eggeling
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