The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Bisphosphonate nanoclay edge-site interactions facilitate hydrogel self-assembly and sustained growth factor localization

Bisphosphonate nanoclay edge-site interactions facilitate hydrogel self-assembly and sustained growth factor localization
Bisphosphonate nanoclay edge-site interactions facilitate hydrogel self-assembly and sustained growth factor localization
Nanoclays have generated interest in biomaterial design for their ability to enhance the mechanics of polymeric materials and impart biological function. As well as their utility as physical cross-linkers, clays have been explored for sustained localization of biomolecules to promote in vivo tissue regeneration. To date, both biomolecule-clay and polymer-clay nanocomposite strategies have utilised the negatively charged clay particle surface. As such, biomolecule-clay and polymer-clay interactions are set in competition, potentially limiting the functional enhancements achieved. Here, we apply specific bisphosphonate interactions with the positively charged clay particle edge to develop self-assembling hydrogels and functionalized clay nanoparticles with preserved surface exchange capacity. Low concentrations of nanoclay are applied to cross-link hyaluronic acid polymers derivatised with a pendant bisphosphonate to generate hydrogels with enhanced mechanical properties and preserved protein binding able to sustain, for over six weeks in vivo, the localized activity of the clinically licensed growth factor BMP-2.
2041-1723
Kim, Yanghee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d
Yang, Xia
a9c00f99-add2-45d0-9b50-3d1c82de3af3
Shi, Liyang
cb0cdc3f-9a7b-4b18-b4c7-0f67c09b89e4
Lanham, Stuart
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Hilborn, Jons
a62f485f-ca16-43fd-bc42-655de173ab08
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Ossipov, Dmitri
06c7f8d4-7337-4404-bde1-151dccb18c26
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3
Kim, Yanghee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d
Yang, Xia
a9c00f99-add2-45d0-9b50-3d1c82de3af3
Shi, Liyang
cb0cdc3f-9a7b-4b18-b4c7-0f67c09b89e4
Lanham, Stuart
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Hilborn, Jons
a62f485f-ca16-43fd-bc42-655de173ab08
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Ossipov, Dmitri
06c7f8d4-7337-4404-bde1-151dccb18c26
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3

Kim, Yanghee, Yang, Xia, Shi, Liyang, Lanham, Stuart, Hilborn, Jons, Oreffo, Richard, Ossipov, Dmitri and Dawson, Jonathan (2020) Bisphosphonate nanoclay edge-site interactions facilitate hydrogel self-assembly and sustained growth factor localization. Nature Communications, 11 (1), [1365]. (doi:10.1038/s41467-020-15152-9).

Record type: Article

Abstract

Nanoclays have generated interest in biomaterial design for their ability to enhance the mechanics of polymeric materials and impart biological function. As well as their utility as physical cross-linkers, clays have been explored for sustained localization of biomolecules to promote in vivo tissue regeneration. To date, both biomolecule-clay and polymer-clay nanocomposite strategies have utilised the negatively charged clay particle surface. As such, biomolecule-clay and polymer-clay interactions are set in competition, potentially limiting the functional enhancements achieved. Here, we apply specific bisphosphonate interactions with the positively charged clay particle edge to develop self-assembling hydrogels and functionalized clay nanoparticles with preserved surface exchange capacity. Low concentrations of nanoclay are applied to cross-link hyaluronic acid polymers derivatised with a pendant bisphosphonate to generate hydrogels with enhanced mechanical properties and preserved protein binding able to sustain, for over six weeks in vivo, the localized activity of the clinically licensed growth factor BMP-2.

Text
Manuscript NCOMMS-226505 FIN ACCEPTED Kim et al 2020 - Accepted Manuscript
Download (3MB)

More information

Accepted/In Press date: 21 January 2020
e-pub ahead of print date: 13 March 2020

Identifiers

Local EPrints ID: 438546
URI: http://eprints.soton.ac.uk/id/eprint/438546
ISSN: 2041-1723
PURE UUID: 545c6f0e-4383-45e2-ac5c-7b7db9a7e628
ORCID for Stuart Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Jonathan Dawson: ORCID iD orcid.org/0000-0002-6712-0598

Catalogue record

Date deposited: 16 Mar 2020 17:30
Last modified: 26 Nov 2021 02:53

Export record

Altmetrics

Contributors

Author: Yanghee Kim
Author: Xia Yang
Author: Liyang Shi
Author: Stuart Lanham ORCID iD
Author: Jons Hilborn
Author: Richard Oreffo ORCID iD
Author: Dmitri Ossipov
Author: Jonathan Dawson ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×