Butement, Jonathan, Holloway, Paul, Welsh, Joshua A, Holloway, Judith, Englyst, Nicola, Horak, Peter, West, Jonathan and Wilkinson, James S (2020) Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering. Lab on a Chip, 20 (7), 1267-1280. (doi:10.1039/C9LC01182J).
Abstract
Size measurement of extracellular vesicles is hampered by the high cost and measurement uncertainty of conventional flow cytometers which is mainly due to the use of non-specialised free space optics. Integrated cytometry, where the optics and fluidics are embedded in a monolithic chip shows promise for the production of low cost, micro-flow cytometers dedicated for extracellular vesicle (EV) analysis with improved size measurement accuracy and precision. This research demonstrates a unique integrated cytometer for sub-micron particle size measurement using multi-angle scattering analysis. A combination of three technologies is used: (i) Dean-based hydrodynamic focussing to deliver a tight sample core stream to the analysis region, (ii) integrated waveguides with multimode interference devices to focus a narrow excitation beam onto the sample stream, and (iii) an angular array of collection waveguides to measure particle scattering distribution and calculate diameter. Low index 200 nm liposomes could be detected and polystyrene size standards as small as 400 nm diameter could be measured with an uncertainty of ± 21 nm (1/2 IQR) demonstrating a first step on the path to high performance integrated cytometry of EVs.
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- Faculties (pre 2018 reorg) > Faculty of Medicine (pre 2018 reorg) > Human Development & Health (pre 2018 reorg) > Human Nutrition & Metabolism (pre 2018 reorg)
Current Faculties > Faculty of Medicine > Human Development and Health > Human Development & Health (pre 2018 reorg) > Human Nutrition & Metabolism (pre 2018 reorg)
Human Development and Health > Human Development & Health (pre 2018 reorg) > Human Nutrition & Metabolism (pre 2018 reorg) - Faculties (pre 2018 reorg) > Faculty of Medicine (pre 2018 reorg) > Human Development & Health (pre 2018 reorg)
Current Faculties > Faculty of Medicine > Human Development and Health > Human Development & Health (pre 2018 reorg)
Human Development and Health > Human Development & Health (pre 2018 reorg) - Current Faculties > Faculty of Engineering and Physical Sciences > Zepler Institute for Photonics and Nanoelectronics > Nanophotonics Group > Photonic Systems Circuits and Sensors Group
Zepler Institute for Photonics and Nanoelectronics > Nanophotonics Group > Photonic Systems Circuits and Sensors Group - Faculties (pre 2018 reorg) > Faculty of Natural and Environmental Sciences (pre 2018 reorg) > Institute for Life Sciences (pre 2018 reorg)
Current Faculties > Faculty of Environmental and Life Sciences > Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg)
Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg) - Current Faculties > Faculty of Medicine > Clinical and Experimental Sciences > Respiratory
Clinical and Experimental Sciences > Respiratory - Current Faculties > Faculty of Engineering and Physical Sciences > Zepler Institute for Photonics and Nanoelectronics > Nanophotonics Group
Zepler Institute for Photonics and Nanoelectronics > Nanophotonics Group - Current Faculties > Faculty of Engineering and Physical Sciences > Zepler Institute for Photonics and Nanoelectronics > Fibre and Systems Group
Zepler Institute for Photonics and Nanoelectronics > Fibre and Systems Group - Current Faculties > Faculty of Medicine > Cancer Sciences
Cancer Sciences - Current Faculties > Faculty of Medicine > Human Development and Health
Human Development and Health - Faculties (pre 2018 reorg) > Faculty of Medicine (pre 2018 reorg) > Cancer Sciences (pre 2018 reorg)
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