A single monoclonal antibody against the peanut allergen Ara H 2 protects against systemic and local peanut allergy
A single monoclonal antibody against the peanut allergen Ara H 2 protects against systemic and local peanut allergy
Background: Peanut allergy is the most prevalent and dangerous food allergy. Peanuts consist of a large number of different allergens and peanut-allergic patients are frequently sensitized to multiple allergens. Hence, conventional desensitization approaches aim at targeting as many allergens as possible. Methods: The monoclonal anti-Ara h 2 antibody (mAb) was produced by hybridoma cells derived from WT BALB/c mice after immunization with a vaccine based on virus-like particles coupled to Ara h 2. BALB/c mice were sensitized intraperitoneally with peanut extract absorbed to alum and mAbs were applied i.v. Challenge was performed the next day with the whole peanut extract intravenously and via skin prick test. Results: Here we show in peanut-allergic mice that a single high-affinity mAb specific for Ara h 2 is able to block systemic and local allergic reactions induced by the complex peanut extract. We confirm in vitro binding of the mAb to the inhibitory low-affinity FcγRIIb receptor using a sensitive biosensor and demonstrate in vivo that protection was dependent on FcγRIIb. Conclusion: A single mAb specific for Ara h 2 is able to improve local and systemic allergic symptoms induced by the whole allergen mixture.
Antibody therapy, FcγRIIb dependency, Food allergy
334-341
Cragg, Mark
ec97f80e-f3c8-49b7-a960-20dff648b78c
1 May 2020
Cragg, Mark
ec97f80e-f3c8-49b7-a960-20dff648b78c
Cragg, Mark
(2020)
A single monoclonal antibody against the peanut allergen Ara H 2 protects against systemic and local peanut allergy.
International Archives of Allergy and Immunology, 181 (5), .
(doi:10.1159/000505917).
Abstract
Background: Peanut allergy is the most prevalent and dangerous food allergy. Peanuts consist of a large number of different allergens and peanut-allergic patients are frequently sensitized to multiple allergens. Hence, conventional desensitization approaches aim at targeting as many allergens as possible. Methods: The monoclonal anti-Ara h 2 antibody (mAb) was produced by hybridoma cells derived from WT BALB/c mice after immunization with a vaccine based on virus-like particles coupled to Ara h 2. BALB/c mice were sensitized intraperitoneally with peanut extract absorbed to alum and mAbs were applied i.v. Challenge was performed the next day with the whole peanut extract intravenously and via skin prick test. Results: Here we show in peanut-allergic mice that a single high-affinity mAb specific for Ara h 2 is able to block systemic and local allergic reactions induced by the complex peanut extract. We confirm in vitro binding of the mAb to the inhibitory low-affinity FcγRIIb receptor using a sensitive biosensor and demonstrate in vivo that protection was dependent on FcγRIIb. Conclusion: A single mAb specific for Ara h 2 is able to improve local and systemic allergic symptoms induced by the whole allergen mixture.
Text
Peanut_mAb_International_Archives_FS_PE_MVMSC
- Accepted Manuscript
More information
e-pub ahead of print date: 10 March 2020
Published date: 1 May 2020
Additional Information:
© 2020 The Author(s) Published by S. Karger AG, Basel.
Keywords:
Antibody therapy, FcγRIIb dependency, Food allergy
Identifiers
Local EPrints ID: 438847
URI: http://eprints.soton.ac.uk/id/eprint/438847
ISSN: 1018-2438
PURE UUID: 6ccdc9bf-1bf6-437c-aed8-7f06c3b9c173
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Date deposited: 25 Mar 2020 17:31
Last modified: 17 Mar 2024 05:25
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