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A single monoclonal antibody against the peanut allergen Ara H 2 protects against systemic and local peanut allergy

A single monoclonal antibody against the peanut allergen Ara H 2 protects against systemic and local peanut allergy
A single monoclonal antibody against the peanut allergen Ara H 2 protects against systemic and local peanut allergy
Background: peanut allergy is the most prevalent and dangerous food allergy. Peanuts consist of a large number of different allergens and peanut-allergic patients are frequently sensitized to multiple allergens. Hence, conventional de-sensitization approaches aim at targeting as many allergens as possible.

Methods: the monoclonal anti-Ara h 2 antibody (mAb) was produced by hybridoma cells derived from WT BALB/c mice after immunization with a vaccine based on Virus-Like-Particles coupled to Ara h 2. BALB/c mice were sensitized intraperitoneally with peanut extract absorbed to Alum and mAbs were applied i.v.. Challenge was performed the next day with the whole peanut extract intravenously and via skin prick test.

Results: here we show in peanut-allergic mice that a single high-affinity monoclonal antibody specific for Ara h 2 is able to block systemic and local allergic reactions induced by the complex peanut extract. We confirm in vitro binding of the mAb to the inhibitory low-affinity FcRIIb receptor using a sensitive biosensor and demonstrate in vivo that protection was dependent on FcRIIb.

Conclusion: a single mAb specific for Ara h 2 is able to improve local and systemic allergic symptoms induced by the whole allergen mixture.
Antibody therapy, FcγRIIb dependency, Food allergy
1018-2438
334-341
Cragg, Mark
ec97f80e-f3c8-49b7-a960-20dff648b78c
Cragg, Mark
ec97f80e-f3c8-49b7-a960-20dff648b78c

Cragg, Mark (2020) A single monoclonal antibody against the peanut allergen Ara H 2 protects against systemic and local peanut allergy. International Archives of Allergy and Immunology, 181 (5), 334-341. (doi:10.1159/000505917).

Record type: Article

Abstract

Background: peanut allergy is the most prevalent and dangerous food allergy. Peanuts consist of a large number of different allergens and peanut-allergic patients are frequently sensitized to multiple allergens. Hence, conventional de-sensitization approaches aim at targeting as many allergens as possible.

Methods: the monoclonal anti-Ara h 2 antibody (mAb) was produced by hybridoma cells derived from WT BALB/c mice after immunization with a vaccine based on Virus-Like-Particles coupled to Ara h 2. BALB/c mice were sensitized intraperitoneally with peanut extract absorbed to Alum and mAbs were applied i.v.. Challenge was performed the next day with the whole peanut extract intravenously and via skin prick test.

Results: here we show in peanut-allergic mice that a single high-affinity monoclonal antibody specific for Ara h 2 is able to block systemic and local allergic reactions induced by the complex peanut extract. We confirm in vitro binding of the mAb to the inhibitory low-affinity FcRIIb receptor using a sensitive biosensor and demonstrate in vivo that protection was dependent on FcRIIb.

Conclusion: a single mAb specific for Ara h 2 is able to improve local and systemic allergic symptoms induced by the whole allergen mixture.

Text
Peanut_mAb_International_Archives_FS_PE_MVMSC - Accepted Manuscript
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More information

e-pub ahead of print date: 10 March 2020
Keywords: Antibody therapy, FcγRIIb dependency, Food allergy

Identifiers

Local EPrints ID: 438847
URI: http://eprints.soton.ac.uk/id/eprint/438847
ISSN: 1018-2438
PURE UUID: 6ccdc9bf-1bf6-437c-aed8-7f06c3b9c173
ORCID for Mark Cragg: ORCID iD orcid.org/0000-0003-2077-089X

Catalogue record

Date deposited: 25 Mar 2020 17:31
Last modified: 26 Nov 2021 06:14

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