Three-dimensional materials made from engineered oligonucleotides and nanoparticles
Three-dimensional materials made from engineered oligonucleotides and nanoparticles
In the last decades, the ability to assemble nanoparticles into programmed 2D or 3D structures by means of synthetic oligonucleotides has resulted into the fabrication of novel nanomaterials with unique physical and chemical properties. Nuclei acids are an exceptional platform for the architecture of complex structures, allowing routes to flexible and precise placing of functional nanoparticles. The physicochemical properties of featured materials can be readily tuned by varying the constituent building blocks.
In this thesis, the advanced programming of the assembly processes was combined with the use of innovative ligation techniques. DNA-functionalised nanoparticles of different composition, size and shape were prepared and used in DNA-guided assembling processes (Chapter 3).
Furthermore, engineered synthetic DNA strands were designed and employed for the fabrication of nanoparticle assemblies with stability and resistance against DNA denaturing conditions (Chapter 4). DNA ligation was realised using a UV-sensitive molecule, the 3-cyanovinyl carbazole. Following light-stimulation, this compound allows the formation of an interstrand crosslink within a DNA duplex, providing a novel tool for the manipulation of three-dimensional nanoparticles assemblies. In contrast with other crosslinking techniques, the carbazole interstrand bond can be reversed, using a different wavelength as an external trigger. The efficient application of the 3-cyanovinyl carbazole in extended nanoparticle crystals is demonstrated for the first time.
DNA-directed heterogeneous assemblies of plasmonic and fluorescent nanoparticles were also manufactured (Chapter 5). The interplay between gold and upconversion nanoparticles within the same aggregate structure and the effect on the linear and non-linear optical properties was studied.
University of Southampton
De Fazio, Angela Federica
cb7894d0-7bd8-41c9-97b2-b3e4f6d44d04
September 2019
De Fazio, Angela Federica
cb7894d0-7bd8-41c9-97b2-b3e4f6d44d04
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
De Fazio, Angela Federica
(2019)
Three-dimensional materials made from engineered oligonucleotides and nanoparticles.
University of Southampton, Doctoral Thesis, 201pp.
Record type:
Thesis
(Doctoral)
Abstract
In the last decades, the ability to assemble nanoparticles into programmed 2D or 3D structures by means of synthetic oligonucleotides has resulted into the fabrication of novel nanomaterials with unique physical and chemical properties. Nuclei acids are an exceptional platform for the architecture of complex structures, allowing routes to flexible and precise placing of functional nanoparticles. The physicochemical properties of featured materials can be readily tuned by varying the constituent building blocks.
In this thesis, the advanced programming of the assembly processes was combined with the use of innovative ligation techniques. DNA-functionalised nanoparticles of different composition, size and shape were prepared and used in DNA-guided assembling processes (Chapter 3).
Furthermore, engineered synthetic DNA strands were designed and employed for the fabrication of nanoparticle assemblies with stability and resistance against DNA denaturing conditions (Chapter 4). DNA ligation was realised using a UV-sensitive molecule, the 3-cyanovinyl carbazole. Following light-stimulation, this compound allows the formation of an interstrand crosslink within a DNA duplex, providing a novel tool for the manipulation of three-dimensional nanoparticles assemblies. In contrast with other crosslinking techniques, the carbazole interstrand bond can be reversed, using a different wavelength as an external trigger. The efficient application of the 3-cyanovinyl carbazole in extended nanoparticle crystals is demonstrated for the first time.
DNA-directed heterogeneous assemblies of plasmonic and fluorescent nanoparticles were also manufactured (Chapter 5). The interplay between gold and upconversion nanoparticles within the same aggregate structure and the effect on the linear and non-linear optical properties was studied.
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Three-dimensional materials made from engineered oligonucleotides and nanoparticles
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Published date: September 2019
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Local EPrints ID: 438903
URI: http://eprints.soton.ac.uk/id/eprint/438903
PURE UUID: 7280297e-ef9b-40d5-b2eb-7245363021ff
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Date deposited: 26 Mar 2020 17:31
Last modified: 17 Mar 2024 03:12
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Author:
Angela Federica De Fazio
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