The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Design and fabrication of optical flow cell for multiplex detection of β-lactamase in microchannels

Design and fabrication of optical flow cell for multiplex detection of β-lactamase in microchannels
Design and fabrication of optical flow cell for multiplex detection of β-lactamase in microchannels
Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip.
microfluidics; point-of-care (POC) diagnostics; antimicrobial resistance (AMR); lab-on-a-chip; absorbance; optical detections; linear actuators; beta-lactamase; analytical chemistry
2072-666X
1-10
Hassan, Sammer-Ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Hassan, Sammer-Ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1

Hassan, Sammer-Ul and Zhang, Xunli (2020) Design and fabrication of optical flow cell for multiplex detection of β-lactamase in microchannels. Micromachines, 11 (4), 1-10, [385]. (doi:10.3390/mi11040385).

Record type: Article

Abstract

Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip.

Text
micromachines-11-00385-1 - Version of Record
Available under License Creative Commons Attribution.
Download (2MB)

More information

Accepted/In Press date: 3 April 2020
Published date: 5 April 2020
Keywords: microfluidics; point-of-care (POC) diagnostics; antimicrobial resistance (AMR); lab-on-a-chip; absorbance; optical detections; linear actuators; beta-lactamase; analytical chemistry

Identifiers

Local EPrints ID: 439174
URI: http://eprints.soton.ac.uk/id/eprint/439174
ISSN: 2072-666X
PURE UUID: c06cabde-ced9-46d0-82b9-616200e3cb72
ORCID for Sammer-Ul Hassan: ORCID iD orcid.org/0000-0002-0319-5814
ORCID for Xunli Zhang: ORCID iD orcid.org/0000-0002-4375-1571

Catalogue record

Date deposited: 06 Apr 2020 16:31
Last modified: 26 Nov 2021 02:52

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×