Design and fabrication of optical flow cell for multiplex detection of β-lactamase in microchannels
Design and fabrication of optical flow cell for multiplex detection of β-lactamase in microchannels
Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip.
microfluidics; point-of-care (POC) diagnostics; antimicrobial resistance (AMR); lab-on-a-chip; absorbance; optical detections; linear actuators; beta-lactamase; analytical chemistry
1-10
Hassan, Sammer-Ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
5 April 2020
Hassan, Sammer-Ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Hassan, Sammer-Ul and Zhang, Xunli
(2020)
Design and fabrication of optical flow cell for multiplex detection of β-lactamase in microchannels.
Micromachines, 11 (4), , [385].
(doi:10.3390/mi11040385).
Abstract
Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip.
Text
micromachines-11-00385-1
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Accepted/In Press date: 3 April 2020
Published date: 5 April 2020
Additional Information:
Funding Information:
Funding: This research was funded by Economic Social Science Research Council, grant number ES/S000208/1.
Funding Information:
Acknowledgments: The presented study was done as part of the DOSA Project (Diagnostics for One Health and User Driven Solutions for AMR, www.dosa-diagnostics.org.) DOSA is funded by UK Research and Innovation /Economic Social Science Research Council, Newton Fund and the Government of India’s Department of Biotechnology.
Publisher Copyright:
© 2020 by the authors.
Keywords:
microfluidics; point-of-care (POC) diagnostics; antimicrobial resistance (AMR); lab-on-a-chip; absorbance; optical detections; linear actuators; beta-lactamase; analytical chemistry
Identifiers
Local EPrints ID: 439174
URI: http://eprints.soton.ac.uk/id/eprint/439174
ISSN: 2072-666X
PURE UUID: c06cabde-ced9-46d0-82b9-616200e3cb72
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Date deposited: 06 Apr 2020 16:31
Last modified: 17 Mar 2024 03:10
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