The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Effects of temperature and nutrient supply on resource allocation, photosynthetic strategy, and metabolic rates of Synechococcus sp.

Effects of temperature and nutrient supply on resource allocation, photosynthetic strategy, and metabolic rates of Synechococcus sp.
Effects of temperature and nutrient supply on resource allocation, photosynthetic strategy, and metabolic rates of Synechococcus sp.
Temperature and nutrient supply are key factors that control phytoplankton ecophysiology, but their role is commonly investigated in isolation. Their combined effect on resource allocation, photosynthetic strategy, and metabolism remains poorly understood. To characterize the photosynthetic strategy and resource allocation under different conditions, we analyzed the responses of a marine cyanobacterium (Synechococcus PCC 7002) to multiple combinations of temperature and nutrient supply. We measured the abundance of proteins involved in the dark (RuBisCO, rbcL) and light (Photosystem II, psbA) photosynthetic reactions, the content of chlorophyll a, carbon and nitrogen, and the rates of photosynthesis, respiration, and growth. We found that rbcL and psbA abundance increased with nutrient supply, whereas a temperature‐induced increase in psbA occurred only in nutrient‐replete treatments. Low temperature and abundant nutrients caused increased RuBisCO abundance, a pattern we observed also in natural phytoplankton assemblages across a wide latitudinal range. Photosynthesis and respiration increased with temperature only under nutrient‐sufficient conditions. These results suggest that nutrient supply exerts a stronger effect than temperature upon both photosynthetic protein abundance and metabolic rates in Synechococcus sp. and that the temperature effect on photosynthetic physiology and metabolism is nutrient dependent. The preferential resource allocation into the light instead of the dark reactions of photosynthesis as temperature rises is likely related to the different temperature dependence of dark‐reaction enzymatic rates versus photochemistry. These findings contribute to our understanding of the strategies for photosynthetic energy allocation in phytoplankton inhabiting contrasting environments.
D1 (PsbA) protein of PSII, RuBisCO, activation energy, metabolic rates, nutrient supply, photosynthetic strategy, temperature
1529-8817
818-829
Fernández-González, Cristina
8d96ad7d-91df-4562-9b95-32d07884601f
Pérez-Lorenzo, María
2d744955-4e8c-4a87-b192-707750b40ef6
Pratt, Nicola
c94f98bd-897c-4853-bebd-be93b8aecc8a
Moore, Christopher
7ec80b7b-bedc-4dd5-8924-0f5d01927b12
Bibby, Thomas
e04ea079-dd90-4ead-9840-00882de27ebd
Marañón, Emilio
c1799c8b-0849-400f-88c3-7ba064feff5c
Fernández-González, Cristina
8d96ad7d-91df-4562-9b95-32d07884601f
Pérez-Lorenzo, María
2d744955-4e8c-4a87-b192-707750b40ef6
Pratt, Nicola
c94f98bd-897c-4853-bebd-be93b8aecc8a
Moore, Christopher
7ec80b7b-bedc-4dd5-8924-0f5d01927b12
Bibby, Thomas
e04ea079-dd90-4ead-9840-00882de27ebd
Marañón, Emilio
c1799c8b-0849-400f-88c3-7ba064feff5c

Fernández-González, Cristina, Pérez-Lorenzo, María, Pratt, Nicola, Moore, Christopher, Bibby, Thomas and Marañón, Emilio (2020) Effects of temperature and nutrient supply on resource allocation, photosynthetic strategy, and metabolic rates of Synechococcus sp. Journal of Phycology, 56 (3), 818-829. (doi:10.1111/jpy.12983).

Record type: Article

Abstract

Temperature and nutrient supply are key factors that control phytoplankton ecophysiology, but their role is commonly investigated in isolation. Their combined effect on resource allocation, photosynthetic strategy, and metabolism remains poorly understood. To characterize the photosynthetic strategy and resource allocation under different conditions, we analyzed the responses of a marine cyanobacterium (Synechococcus PCC 7002) to multiple combinations of temperature and nutrient supply. We measured the abundance of proteins involved in the dark (RuBisCO, rbcL) and light (Photosystem II, psbA) photosynthetic reactions, the content of chlorophyll a, carbon and nitrogen, and the rates of photosynthesis, respiration, and growth. We found that rbcL and psbA abundance increased with nutrient supply, whereas a temperature‐induced increase in psbA occurred only in nutrient‐replete treatments. Low temperature and abundant nutrients caused increased RuBisCO abundance, a pattern we observed also in natural phytoplankton assemblages across a wide latitudinal range. Photosynthesis and respiration increased with temperature only under nutrient‐sufficient conditions. These results suggest that nutrient supply exerts a stronger effect than temperature upon both photosynthetic protein abundance and metabolic rates in Synechococcus sp. and that the temperature effect on photosynthetic physiology and metabolism is nutrient dependent. The preferential resource allocation into the light instead of the dark reactions of photosynthesis as temperature rises is likely related to the different temperature dependence of dark‐reaction enzymatic rates versus photochemistry. These findings contribute to our understanding of the strategies for photosynthetic energy allocation in phytoplankton inhabiting contrasting environments.

Text
Fernandez-Gonzalez_et_al._Accepted_pdf - Accepted Manuscript
Download (501kB)

More information

Accepted/In Press date: 23 February 2020
e-pub ahead of print date: 4 March 2020
Published date: 1 June 2020
Additional Information: Funding Information: This research was supported by the Spanish Ministry of Science and Innovation through research grant PGC2018-094553-B-I00 to E. M. We thank the scientific complement and crew of the RRS James Cook, RVIB Nathaniel B. Palmer and RRS Discovery during AMT-19, NBP12-01, D350 and D354, respectively, for all of their assistance. These cruises were supported by grants from the National Science Foundation, USA (ANT-0944254) and National Environmental Research Council, UK (NE/F019254/1 and NE/G009155/1). C. F.-G. acknowledges the receipt of a predoctoral research fellowship from Xunta de Galicia. We thank ?ngeles Saavedra for advice with statistical analyses and M. J. Cabrerizo for comments on an earlier version of the manuscript. Publisher Copyright: © 2020 Phycological Society of America Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
Keywords: D1 (PsbA) protein of PSII, RuBisCO, activation energy, metabolic rates, nutrient supply, photosynthetic strategy, temperature

Identifiers

Local EPrints ID: 439414
URI: http://eprints.soton.ac.uk/id/eprint/439414
ISSN: 1529-8817
PURE UUID: 5f0746b1-3300-4ea8-9344-e589602ad7bb
ORCID for Nicola Pratt: ORCID iD orcid.org/0000-0002-0664-3467
ORCID for Christopher Moore: ORCID iD orcid.org/0000-0002-9541-6046

Catalogue record

Date deposited: 22 Apr 2020 16:30
Last modified: 26 Nov 2021 06:56

Export record

Altmetrics

Contributors

Author: Cristina Fernández-González
Author: María Pérez-Lorenzo
Author: Nicola Pratt ORCID iD
Author: Thomas Bibby
Author: Emilio Marañón

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×