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SAR11 dominance among metabolically active low nucleic acid bacterioplankton in surface waters along an Atlantic Meridional Transect

SAR11 dominance among metabolically active low nucleic acid bacterioplankton in surface waters along an Atlantic Meridional Transect
SAR11 dominance among metabolically active low nucleic acid bacterioplankton in surface waters along an Atlantic Meridional Transect
Low nucleic acid (LNA) bacterioplankton (sorted by flow cytometry) were characterised in surface water samples along a meridional transect from 48°N to 40° S across the Atlantic Ocean. The LNA bacterioplankton abundance and metabolic activity, assessed by their 35S-methionine uptake rate, were similar along the transect, representing 36 ± 6 and 36 ± 11% of total bacterioplankton, respectively. Fluorescence in situ hybridisation analysis of the flow-sorted cells revealed that the LNA bacterioplankton population was dominated (59 ± 4%) by and contained virtually all the identifiable SAR11 clade cells throughout the Atlantic Ocean. Therefore, the present study provides ecological characterisation of this flow-sorted group and suggests both phylogenetic and functional constancy of the LNA bacterioplankton at the basin-scale.
flow cytometry sorting, cell metabolic activity, SAR11 clade, CARD-FISH, radioactive tracer labelling, Atlantic Ocean
0948-3055
107-113
Mary, I.
759ba210-d47f-4199-b0cf-27e585371ca9
Heywood, J.L.
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Fuchs, B.M.
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Amann, R.
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Tarran, G.A.
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Burkill, P.H.
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Zubkov, M.V.
b1dfb3a0-bcff-430c-9031-358a22b50743
Mary, I.
759ba210-d47f-4199-b0cf-27e585371ca9
Heywood, J.L.
4d5bb933-4925-4b14-9db3-bd0c7da927d4
Fuchs, B.M.
1edd27c8-6b61-47f1-9c65-788d3fef9fd3
Amann, R.
c56b595a-e2d2-43f4-b058-a34b5fcf05f8
Tarran, G.A.
c6e9fb51-321c-4fb6-a2b0-00a58c344d73
Burkill, P.H.
91175019-8b55-4fb5-84ea-334c12de2557
Zubkov, M.V.
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Mary, I., Heywood, J.L., Fuchs, B.M., Amann, R., Tarran, G.A., Burkill, P.H. and Zubkov, M.V. (2006) SAR11 dominance among metabolically active low nucleic acid bacterioplankton in surface waters along an Atlantic Meridional Transect. Aquatic Microbial Ecology, 45 (2), 107-113.

Record type: Article

Abstract

Low nucleic acid (LNA) bacterioplankton (sorted by flow cytometry) were characterised in surface water samples along a meridional transect from 48°N to 40° S across the Atlantic Ocean. The LNA bacterioplankton abundance and metabolic activity, assessed by their 35S-methionine uptake rate, were similar along the transect, representing 36 ± 6 and 36 ± 11% of total bacterioplankton, respectively. Fluorescence in situ hybridisation analysis of the flow-sorted cells revealed that the LNA bacterioplankton population was dominated (59 ± 4%) by and contained virtually all the identifiable SAR11 clade cells throughout the Atlantic Ocean. Therefore, the present study provides ecological characterisation of this flow-sorted group and suggests both phylogenetic and functional constancy of the LNA bacterioplankton at the basin-scale.

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More information

Published date: 2006
Keywords: flow cytometry sorting, cell metabolic activity, SAR11 clade, CARD-FISH, radioactive tracer labelling, Atlantic Ocean

Identifiers

Local EPrints ID: 43952
URI: http://eprints.soton.ac.uk/id/eprint/43952
ISSN: 0948-3055
PURE UUID: 4be898a8-219f-4c15-8e13-0ac4d213b6fe

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Date deposited: 02 Feb 2007
Last modified: 08 Jan 2022 01:08

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Contributors

Author: I. Mary
Author: J.L. Heywood
Author: B.M. Fuchs
Author: R. Amann
Author: G.A. Tarran
Author: P.H. Burkill
Author: M.V. Zubkov

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