Design and fabrication of capillary-driven flow device for point-of-care diagnostics
Design and fabrication of capillary-driven flow device for point-of-care diagnostics
Point-of-care (POC) diagnostics enables the diagnosis and monitoring of patients from the clinic or their home. Ideally, POC devices should be compact, portable and operatable without the requirement of expertise or complex fluid mechanical controls. This paper showcases a chip-and-dip device, which works on the principle of capillary-driven flow microfluidics and allows analytes’ detection by multiple microchannels in a single microchip via smartphone imaging. The chip-and-dip device, fabricated with inexpensive materials, works by simply dipping the reagents-coated microchip consisting of microchannels into a fluidic sample. The sample is loaded into the microchannels via capillary action and reacts with the reagents to produce a colourimetric signal. Unlike dipstick tests, this device allows the loading of bacterial/pathogenic samples for antimicrobial testing. A single device can be coated with multiple reagents, and more analytes can be detected in one sample. This platform could be used for a wide variety of assays. Here, we show the design, fabrication and working principle of the chip-and-dip flow device along with a specific application consisting in the determination of β-lactamase activity and cortisol. The simplicity, robustness and multiplexing capability of the chip-and-dip device will allow it to be used for POC diagnostics.
analytical chemistry, capillary-driven flow, colorimetry, lab-on-a-chip, microfluidics, optical detections, point-of-care (POC) diagnostics, β-lactamase
Hassan, Sammer-Ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
15 April 2020
Hassan, Sammer-Ul
8a5ae3f1-3451-4093-879e-85f40953da8b
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Hassan, Sammer-Ul and Zhang, Xunli
(2020)
Design and fabrication of capillary-driven flow device for point-of-care diagnostics.
Biosensors, 10 (4), [39].
(doi:10.3390/bios10040039).
Abstract
Point-of-care (POC) diagnostics enables the diagnosis and monitoring of patients from the clinic or their home. Ideally, POC devices should be compact, portable and operatable without the requirement of expertise or complex fluid mechanical controls. This paper showcases a chip-and-dip device, which works on the principle of capillary-driven flow microfluidics and allows analytes’ detection by multiple microchannels in a single microchip via smartphone imaging. The chip-and-dip device, fabricated with inexpensive materials, works by simply dipping the reagents-coated microchip consisting of microchannels into a fluidic sample. The sample is loaded into the microchannels via capillary action and reacts with the reagents to produce a colourimetric signal. Unlike dipstick tests, this device allows the loading of bacterial/pathogenic samples for antimicrobial testing. A single device can be coated with multiple reagents, and more analytes can be detected in one sample. This platform could be used for a wide variety of assays. Here, we show the design, fabrication and working principle of the chip-and-dip flow device along with a specific application consisting in the determination of β-lactamase activity and cortisol. The simplicity, robustness and multiplexing capability of the chip-and-dip device will allow it to be used for POC diagnostics.
Text
Design and Fabrication of Capillary-Driven Flow Device Hassan2020
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Accepted/In Press date: 13 April 2020
Published date: 15 April 2020
Additional Information:
Funding Information:
Funding: This research was funded by the Economic Social Science Research Council, grant number ES/S000208/1.
Funding Information:
Acknowledgments: This research was performed as part of the DOSA Project (Diagnostics for One Health and User Driven Solutions for AMR, www.dosa-diagnostics.org. DOSA is funded by UK Research and Innovation/Economic Social Science Research Council, Newton Fund, and the Government of India’s Department of Biotechnology.
Publisher Copyright:
© 2020 by the authors.
Keywords:
analytical chemistry, capillary-driven flow, colorimetry, lab-on-a-chip, microfluidics, optical detections, point-of-care (POC) diagnostics, β-lactamase
Identifiers
Local EPrints ID: 439545
URI: http://eprints.soton.ac.uk/id/eprint/439545
ISSN: 2079-6374
PURE UUID: 63b0bfbd-c41f-4362-b1e2-0797e2055d94
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Date deposited: 27 Apr 2020 16:30
Last modified: 17 Mar 2024 03:10
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