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Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation

Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation
Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation
Consistent mechanical performance from implantation through healing and scaffold degradation is highly desired for tissue-regenerative scaffolds, e.g. when used for vascular grafts. The aim of this study was the paired in vivo mechanical assessment of biostable and fast degrading electrospun polyester-urethane scaffolds to isolate the effects of material degradation and tissue formation after implantation. Biostable and degradable polyester-urethane scaffolds with substantial fibre alignment were manufactured by electrospinning. Scaffold samples were implanted paired in subcutaneous position in rats for 7, 14 and 28 days. Morphology, mechanical properties and tissue ingrowth of the scaffolds were assessed before implantation and after retrieval. Tissue ingrowth after 28 days was 83 ± 10% in the biostable scaffold and 77 ± 4% in the degradable scaffold. For the biostable scaffold, the elastic modulus at 12% strain increased significantly between 7 and 14 days and decreased significantly thereafter in fibre but not in cross-fibre direction. The degradable scaffold exhibited a significant increase in the elastic modulus at 12% strain from 7 to 14 days after which it did not decrease but remained at the same magnitude, both in fibre and in cross-fibre direction. Considering that the degradable scaffold loses its material strength predominantly during the first 14 days of hydrolytic degradation (as observed in our previous in vitro study), the consistency of the elastic modulus of the degradable scaffold after 14 days is an indication that the regenerated tissue construct retains it mechanical properties.
Electrospinning, elastic modulus;, mechanical properties;, soft tissue regeneration;, degradation
2523-3971
Krynauw, Hugo
bca56033-a190-40cc-83a3-176eee16fc50
Omar, Rodaina
db341f39-93b5-4898-b8de-f6b2ed3d0479
Koehne, Josepha
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Limbert, Georges
a1b88cb4-c5d9-4c6e-b6c9-7f4c4aa1c2ec
Davies, Neil
1b142484-f30b-4390-85a9-581cbda11121
Bezuidenhout, Deon
9b2c8bc3-625e-4000-bc02-2be1bcf8710d
Franz, Thomas
fe0873a7-c37b-4db5-99c0-70b62615ba3b
Krynauw, Hugo
bca56033-a190-40cc-83a3-176eee16fc50
Omar, Rodaina
db341f39-93b5-4898-b8de-f6b2ed3d0479
Koehne, Josepha
e676d9c3-49f9-4731-9845-750d69555055
Limbert, Georges
a1b88cb4-c5d9-4c6e-b6c9-7f4c4aa1c2ec
Davies, Neil
1b142484-f30b-4390-85a9-581cbda11121
Bezuidenhout, Deon
9b2c8bc3-625e-4000-bc02-2be1bcf8710d
Franz, Thomas
fe0873a7-c37b-4db5-99c0-70b62615ba3b

Krynauw, Hugo, Omar, Rodaina, Koehne, Josepha, Limbert, Georges, Davies, Neil, Bezuidenhout, Deon and Franz, Thomas (2020) Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation. SN Applied Sciences, 2, [953]. (doi:10.1007/s42452-020-2764-6).

Record type: Article

Abstract

Consistent mechanical performance from implantation through healing and scaffold degradation is highly desired for tissue-regenerative scaffolds, e.g. when used for vascular grafts. The aim of this study was the paired in vivo mechanical assessment of biostable and fast degrading electrospun polyester-urethane scaffolds to isolate the effects of material degradation and tissue formation after implantation. Biostable and degradable polyester-urethane scaffolds with substantial fibre alignment were manufactured by electrospinning. Scaffold samples were implanted paired in subcutaneous position in rats for 7, 14 and 28 days. Morphology, mechanical properties and tissue ingrowth of the scaffolds were assessed before implantation and after retrieval. Tissue ingrowth after 28 days was 83 ± 10% in the biostable scaffold and 77 ± 4% in the degradable scaffold. For the biostable scaffold, the elastic modulus at 12% strain increased significantly between 7 and 14 days and decreased significantly thereafter in fibre but not in cross-fibre direction. The degradable scaffold exhibited a significant increase in the elastic modulus at 12% strain from 7 to 14 days after which it did not decrease but remained at the same magnitude, both in fibre and in cross-fibre direction. Considering that the degradable scaffold loses its material strength predominantly during the first 14 days of hydrolytic degradation (as observed in our previous in vitro study), the consistency of the elastic modulus of the degradable scaffold after 14 days is an indication that the regenerated tissue construct retains it mechanical properties.

Text
SNAS-D-19-07383_R1 - Accepted Manuscript
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More information

Accepted/In Press date: 15 April 2020
Published date: 24 April 2020
Keywords: Electrospinning, elastic modulus;, mechanical properties;, soft tissue regeneration;, degradation

Identifiers

Local EPrints ID: 439720
URI: http://eprints.soton.ac.uk/id/eprint/439720
ISSN: 2523-3971
PURE UUID: 047f1640-efad-4548-b822-d5203b84d7f1

Catalogue record

Date deposited: 30 Apr 2020 16:31
Last modified: 26 Nov 2021 07:16

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Contributors

Author: Hugo Krynauw
Author: Rodaina Omar
Author: Josepha Koehne
Author: Georges Limbert
Author: Neil Davies
Author: Deon Bezuidenhout
Author: Thomas Franz

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