Dysregulated neurovascular control underlies declining microvascular functionality in people with non-alcoholic fatty liver disease (NAFLD) at risk of liver fibrosis
Dysregulated neurovascular control underlies declining microvascular functionality in people with non-alcoholic fatty liver disease (NAFLD) at risk of liver fibrosis
Background/Aims: Increasing evidence shows that non-alcoholic fatty liver disease (NAFLD) is associated with dysregulation of microvascular perfusion independently of established cardio-metabolic risk factors. We investigated whether hepatic manifestations of NAFLD such as liver fibrosis and liver fat are associated with microvascular hemodynamics through dysregulation of neurovascular control. Methods: Microvascular dilator (post-occlusive reactive hyperemia) and sympathetically mediated constrictor (deep inspiratory breath-hold) responses were measured at the forearm and finger, respectively, using laser Doppler fluximetry. Non-linear complexity-based analysis was used to assess the information content and variability of the resting blood flux (BF) signals, attributable to oscillatory flow-motion activity, and over multiple sampling frequencies. Results: Measurements were made in 189 adults (113 men) with NAFLD, with (n = 65) and without (n = 124) type 2 diabetes mellitus (T2DM), age = 50.9 ± 11.7 years (mean ± SD). Microvascular dilator and constrictor capacity were both negatively associated with age (r = −0.178, p = 0.014, and r = −0.201, p = 0.007, respectively) and enhanced liver fibrosis (ELF) score (r = −0.155, p = 0.038 and r = −0.418, p < 0.0001, respectively). There was no association with measures of liver fat, obesity or T2DM. Lempel-Ziv complexity (LZC) and sample entropy (SE) of the BF signal measured at the two skin sites were associated negatively with age (p < 0.01 and p < 0.001) and positively with ELF score (p < 0.05 and p < 0.0001). In individuals with an ELF score ≥7.8 the influence of both neurogenic and respiratory flow-motion activity on LZC was up-rated (p < 0.0001). Conclusion: Altered microvascular network functionality occurs in adults with NAFLD suggesting a mechanistic role for dysregulated neurovascular control in individuals at risk of severe liver fibrosis.
NAFLD, blood flow, flow-motion, liver fibrosis, microcirculation, non-linear complexity analysis, skin, sympathetic nervous system
Clough, Geraldine
9f19639e-a929-4976-ac35-259f9011c494
Chipperfield, Andrew
524269cd-5f30-4356-92d4-891c14c09340
Thanaj, Marjola
fb9baacc-4255-483d-8efa-e4fa983a9b2f
Scorletti, Eleonora
4e896544-2974-4f81-9696-1595d3c36814
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Byrne, Christopher
1370b997-cead-4229-83a7-53301ed2a43c
3 June 2020
Clough, Geraldine
9f19639e-a929-4976-ac35-259f9011c494
Chipperfield, Andrew
524269cd-5f30-4356-92d4-891c14c09340
Thanaj, Marjola
fb9baacc-4255-483d-8efa-e4fa983a9b2f
Scorletti, Eleonora
4e896544-2974-4f81-9696-1595d3c36814
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Byrne, Christopher
1370b997-cead-4229-83a7-53301ed2a43c
Clough, Geraldine, Chipperfield, Andrew, Thanaj, Marjola, Scorletti, Eleonora, Calder, Philip and Byrne, Christopher
(2020)
Dysregulated neurovascular control underlies declining microvascular functionality in people with non-alcoholic fatty liver disease (NAFLD) at risk of liver fibrosis.
Frontiers in Physiology, 11, [551].
(doi:10.3389/fphys.2020.00551).
Abstract
Background/Aims: Increasing evidence shows that non-alcoholic fatty liver disease (NAFLD) is associated with dysregulation of microvascular perfusion independently of established cardio-metabolic risk factors. We investigated whether hepatic manifestations of NAFLD such as liver fibrosis and liver fat are associated with microvascular hemodynamics through dysregulation of neurovascular control. Methods: Microvascular dilator (post-occlusive reactive hyperemia) and sympathetically mediated constrictor (deep inspiratory breath-hold) responses were measured at the forearm and finger, respectively, using laser Doppler fluximetry. Non-linear complexity-based analysis was used to assess the information content and variability of the resting blood flux (BF) signals, attributable to oscillatory flow-motion activity, and over multiple sampling frequencies. Results: Measurements were made in 189 adults (113 men) with NAFLD, with (n = 65) and without (n = 124) type 2 diabetes mellitus (T2DM), age = 50.9 ± 11.7 years (mean ± SD). Microvascular dilator and constrictor capacity were both negatively associated with age (r = −0.178, p = 0.014, and r = −0.201, p = 0.007, respectively) and enhanced liver fibrosis (ELF) score (r = −0.155, p = 0.038 and r = −0.418, p < 0.0001, respectively). There was no association with measures of liver fat, obesity or T2DM. Lempel-Ziv complexity (LZC) and sample entropy (SE) of the BF signal measured at the two skin sites were associated negatively with age (p < 0.01 and p < 0.001) and positively with ELF score (p < 0.05 and p < 0.0001). In individuals with an ELF score ≥7.8 the influence of both neurogenic and respiratory flow-motion activity on LZC was up-rated (p < 0.0001). Conclusion: Altered microvascular network functionality occurs in adults with NAFLD suggesting a mechanistic role for dysregulated neurovascular control in individuals at risk of severe liver fibrosis.
Text
Clough Frontiers accepted version 290420
- Accepted Manuscript
More information
Accepted/In Press date: 30 April 2020
Published date: 3 June 2020
Additional Information:
Funding Information:
We would like to thank the WELCOME and INSYTE Trial Investigators who helped with recruitment and data collection; the research nurses at Southampton General Hospital (Gillian Wise, Bridget Clancy, Sanchia Triggs, Gemma Rood, Andria Staniford, Norma Diaper, and Jennifer Hedges); and Lucinda England for research governance administration. We would also like to thank the WELCOME and INSYTE Trial participants. Funding. This work was supported by the National Institute of Health Research through the NIHR Southampton Biomedical Research Centre and Diabetes United Kingdom (BDA 09/0003937). CB, ES, and PC were supported in part by the Southampton NIHR Biomedical Research Centre. MT was supported by an EPSRC DTP Studentship.
Publisher Copyright:
© Copyright © 2020 Clough, Chipperfield, Thanaj, Scorletti, Calder and Byrne.
Keywords:
NAFLD, blood flow, flow-motion, liver fibrosis, microcirculation, non-linear complexity analysis, skin, sympathetic nervous system
Identifiers
Local EPrints ID: 439758
URI: http://eprints.soton.ac.uk/id/eprint/439758
ISSN: 1664-042X
PURE UUID: 0ff24c12-de44-4d21-8a7b-951951734b36
Catalogue record
Date deposited: 01 May 2020 16:39
Last modified: 17 Mar 2024 02:56
Export record
Altmetrics
Contributors
Author:
Marjola Thanaj
Author:
Eleonora Scorletti
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics