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Photopatterned PLED arrays for biosensing applications

Photopatterned PLED arrays for biosensing applications
Photopatterned PLED arrays for biosensing applications

A solid-state optical biosensor integrated with a single layer flexible blue Polymer Light Emitting Diode (PLED) as the light source was demonstrated and used for the detection of biomolecules labeled with two different fluorescent dyes. An anti-rabbit IgG antibody labeled with the fluorescent dyes, that was adsorbed on the opposite side of a polyethylene tetraphthalate (PET) flexible substrate, was excited by light emitted from the PLED. Light is then absorbed by the dye-labeled antibody resulting in a red shift of the polymer emission spectrum. Emission originating from the dye is also observed. The emission spectral shift and its efficiency depend primarily on the concentration of the biomolecule solution, the overlap between the emission spectra of the polymer and the absorption spectra of the dye and the PLED emission characteristics. Biomolecules immobilized onto distinct areas of a plastic substrate might then selectively be detected with high sensitivity after reacting with fluorescently labeled counterpart molecules.

Dye-labeled biomolecules, Fluorescent probe, Optical sensor, Photopatterned multi-colour PLEDs
0167-9317
1511-1514
Vasilopoulou, Maria
aad1381e-d091-4090-8c7c-b74bed22393d
Georgiadou, Dimitra G.
84977176-3678-4fb3-a3dd-2044a49c853b
Palilis, Leonidas C.
b09e9554-54da-4be5-aa08-bda894e2b86f
Botsialas, Athanasios
b23cb54a-8266-4389-b188-f96c206cd6d2
Petrou, Panagiota S.
3d15fa2f-64e6-4d1d-a569-170855c9c957
Kakabakos, Sotirios E.
35aa15b6-03cf-4a0a-a78c-f4a51c2a4a47
Argitis, Panagiotis
ab9c4ea6-3dd2-4e34-935d-81bfb360f358
Vasilopoulou, Maria
aad1381e-d091-4090-8c7c-b74bed22393d
Georgiadou, Dimitra G.
84977176-3678-4fb3-a3dd-2044a49c853b
Palilis, Leonidas C.
b09e9554-54da-4be5-aa08-bda894e2b86f
Botsialas, Athanasios
b23cb54a-8266-4389-b188-f96c206cd6d2
Petrou, Panagiota S.
3d15fa2f-64e6-4d1d-a569-170855c9c957
Kakabakos, Sotirios E.
35aa15b6-03cf-4a0a-a78c-f4a51c2a4a47
Argitis, Panagiotis
ab9c4ea6-3dd2-4e34-935d-81bfb360f358

Vasilopoulou, Maria, Georgiadou, Dimitra G., Palilis, Leonidas C., Botsialas, Athanasios, Petrou, Panagiota S., Kakabakos, Sotirios E. and Argitis, Panagiotis (2009) Photopatterned PLED arrays for biosensing applications. Microelectronic Engineering, 86 (4-6), 1511-1514. (doi:10.1016/j.mee.2009.01.063).

Record type: Article

Abstract

A solid-state optical biosensor integrated with a single layer flexible blue Polymer Light Emitting Diode (PLED) as the light source was demonstrated and used for the detection of biomolecules labeled with two different fluorescent dyes. An anti-rabbit IgG antibody labeled with the fluorescent dyes, that was adsorbed on the opposite side of a polyethylene tetraphthalate (PET) flexible substrate, was excited by light emitted from the PLED. Light is then absorbed by the dye-labeled antibody resulting in a red shift of the polymer emission spectrum. Emission originating from the dye is also observed. The emission spectral shift and its efficiency depend primarily on the concentration of the biomolecule solution, the overlap between the emission spectra of the polymer and the absorption spectra of the dye and the PLED emission characteristics. Biomolecules immobilized onto distinct areas of a plastic substrate might then selectively be detected with high sensitivity after reacting with fluorescently labeled counterpart molecules.

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More information

Accepted/In Press date: 23 January 2009
e-pub ahead of print date: 1 February 2009
Published date: 1 April 2009
Keywords: Dye-labeled biomolecules, Fluorescent probe, Optical sensor, Photopatterned multi-colour PLEDs

Identifiers

Local EPrints ID: 440493
URI: http://eprints.soton.ac.uk/id/eprint/440493
ISSN: 0167-9317
PURE UUID: 14112664-9182-4cc9-bcec-29419ddccda0
ORCID for Dimitra G. Georgiadou: ORCID iD orcid.org/0000-0002-2620-3346

Catalogue record

Date deposited: 05 May 2020 16:42
Last modified: 07 Oct 2020 02:27

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Contributors

Author: Maria Vasilopoulou
Author: Leonidas C. Palilis
Author: Athanasios Botsialas
Author: Panagiota S. Petrou
Author: Sotirios E. Kakabakos
Author: Panagiotis Argitis

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