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Modelling vaporised hydrogen peroxide efficacy against mono-species biofilms

Modelling vaporised hydrogen peroxide efficacy against mono-species biofilms
Modelling vaporised hydrogen peroxide efficacy against mono-species biofilms

This pilot study investigates a novel approach towards efficacy testing of antimicrobial cleaning agents; focusing primarily on hydrogen peroxide vapour (HPV). Contaminated surfaces are recognised modes of pathogen transmission within healthcare environments and increase the risk of pathogen acquisition in newly admitted patients. Studies have shown these pathogens can survive on surfaces for extended periods of time in spite of cleaning. This resilience is characteristic of biofilm formation and recent publications have identified their presence in hospitals. In this study, biofilm models comprised of multidrug-resistant organisms (MDROs) were generated using a drip flow reactor and exposed to HPV decontamination. The MDROs included Acinetobacter baumannii, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Upon exposure, samples were periodically removed and enumerated to generate kill curves for each species, consequently revealing any inherent resistances; such as catalase-producing organisms which expressed reduced susceptibility. Epifluorescence microscopy revealed an abundance of viable and non-viable microcolonies before and after decontamination, respectively. Greater than 6-Log10 reduction was achieved within a 100 minutes exposure time. This pilot study puts forward a potential methodology for testing antimicrobial agents against biofilms and supports the efficacy of HPV.

82-88
Watson, F.L.
c4c3927e-47bf-4448-a0c2-13a767e28ad8
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Wilks, S.A.
86c1f41a-12b3-451c-9245-b1a21775e993
Chewins, J.
32f2ee49-5dc0-41b2-a174-cad06f7faf14
Watson, F.L.
c4c3927e-47bf-4448-a0c2-13a767e28ad8
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Wilks, S.A.
86c1f41a-12b3-451c-9245-b1a21775e993
Chewins, J.
32f2ee49-5dc0-41b2-a174-cad06f7faf14

Watson, F.L., Keevil, C.W., Wilks, S.A. and Chewins, J. (2018) Modelling vaporised hydrogen peroxide efficacy against mono-species biofilms. European Journal of Parenteral and Pharmaceutical Sciences, 23 (3), 82-88.

Record type: Article

Abstract

This pilot study investigates a novel approach towards efficacy testing of antimicrobial cleaning agents; focusing primarily on hydrogen peroxide vapour (HPV). Contaminated surfaces are recognised modes of pathogen transmission within healthcare environments and increase the risk of pathogen acquisition in newly admitted patients. Studies have shown these pathogens can survive on surfaces for extended periods of time in spite of cleaning. This resilience is characteristic of biofilm formation and recent publications have identified their presence in hospitals. In this study, biofilm models comprised of multidrug-resistant organisms (MDROs) were generated using a drip flow reactor and exposed to HPV decontamination. The MDROs included Acinetobacter baumannii, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Upon exposure, samples were periodically removed and enumerated to generate kill curves for each species, consequently revealing any inherent resistances; such as catalase-producing organisms which expressed reduced susceptibility. Epifluorescence microscopy revealed an abundance of viable and non-viable microcolonies before and after decontamination, respectively. Greater than 6-Log10 reduction was achieved within a 100 minutes exposure time. This pilot study puts forward a potential methodology for testing antimicrobial agents against biofilms and supports the efficacy of HPV.

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More information

Published date: 1 January 2018

Identifiers

Local EPrints ID: 440607
URI: http://eprints.soton.ac.uk/id/eprint/440607
PURE UUID: c177463d-ba14-4517-ae39-e7687813d1bc
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706
ORCID for S.A. Wilks: ORCID iD orcid.org/0000-0002-4134-9415

Catalogue record

Date deposited: 12 May 2020 16:31
Last modified: 13 May 2020 00:31

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