A novel mouse model of Campylobacter jejuni enteropathy and diarrhea
A novel mouse model of Campylobacter jejuni enteropathy and diarrhea
Campylobacter infections are among the leading bacterial causes of diarrhea and of 'environmental enteropathy' (EE) and growth failure worldwide. However, the lack of an inexpensive small animal model of enteric disease with Campylobacter has been a major limitation for understanding its pathogenesis, interventions or vaccine development. We describe a robust standard mouse model that can exhibit reproducible bloody diarrhea or growth failure, depending on the zinc or protein deficient diet and on antibiotic alteration of normal microbiota prior to infection. Zinc deficiency and the use of antibiotics create a niche for Campylobacter infection to establish by narrowing the metabolic flexibility of these mice for pathogen clearance and by promoting intestinal and systemic inflammation. Several biomarkers and intestinal pathology in this model also mimic those seen in human disease. This model provides a novel tool to test specific hypotheses regarding disease pathogenesis as well as vaccine development that is currently in progress.
Animals, Biomarkers/metabolism, Campylobacter Infections/complications, Campylobacter jejuni/pathogenicity, Diarrhea/etiology, Disease Models, Animal, Inflammation/etiology, Intestinal Diseases/etiology, Male, Mice, Mice, Inbred C57BL
1-23
Giallourou, Natasa
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Medlock, Gregory L.
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Bolick, David T.
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Medeiros, Pedro Hqs
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Ledwaba, Solanka E.
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Kolling, Glynis L.
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Tung, Kenneth
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Guerry, Patricia
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Swann, Jonathan R.
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Guerrant, Richard L.
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23 May 2018
Giallourou, Natasa
b5891ea7-98d4-49d7-b883-2c57ca2d962a
Medlock, Gregory L.
41c918e5-fee8-4a6e-a422-e5f09ccc8888
Bolick, David T.
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Medeiros, Pedro Hqs
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Ledwaba, Solanka E.
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Kolling, Glynis L.
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Tung, Kenneth
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Guerry, Patricia
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Swann, Jonathan R.
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Guerrant, Richard L.
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Giallourou, Natasa, Medlock, Gregory L., Bolick, David T., Medeiros, Pedro Hqs, Ledwaba, Solanka E., Kolling, Glynis L., Tung, Kenneth, Guerry, Patricia, Swann, Jonathan R. and Guerrant, Richard L.
(2018)
A novel mouse model of Campylobacter jejuni enteropathy and diarrhea.
PLOS Pathogens, 14 (3), , [e1007083].
(doi:10.1371/journal.ppat.1007083).
Abstract
Campylobacter infections are among the leading bacterial causes of diarrhea and of 'environmental enteropathy' (EE) and growth failure worldwide. However, the lack of an inexpensive small animal model of enteric disease with Campylobacter has been a major limitation for understanding its pathogenesis, interventions or vaccine development. We describe a robust standard mouse model that can exhibit reproducible bloody diarrhea or growth failure, depending on the zinc or protein deficient diet and on antibiotic alteration of normal microbiota prior to infection. Zinc deficiency and the use of antibiotics create a niche for Campylobacter infection to establish by narrowing the metabolic flexibility of these mice for pathogen clearance and by promoting intestinal and systemic inflammation. Several biomarkers and intestinal pathology in this model also mimic those seen in human disease. This model provides a novel tool to test specific hypotheses regarding disease pathogenesis as well as vaccine development that is currently in progress.
Text
journal.ppat.1007083
- Version of Record
More information
Accepted/In Press date: 9 May 2018
Published date: 23 May 2018
Keywords:
Animals, Biomarkers/metabolism, Campylobacter Infections/complications, Campylobacter jejuni/pathogenicity, Diarrhea/etiology, Disease Models, Animal, Inflammation/etiology, Intestinal Diseases/etiology, Male, Mice, Mice, Inbred C57BL
Identifiers
Local EPrints ID: 440708
URI: http://eprints.soton.ac.uk/id/eprint/440708
ISSN: 1553-7366
PURE UUID: d9804cc7-ce4c-4d53-973a-03cb03fc82f2
Catalogue record
Date deposited: 13 May 2020 17:07
Last modified: 17 Mar 2024 04:00
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Contributors
Author:
Natasa Giallourou
Author:
Gregory L. Medlock
Author:
David T. Bolick
Author:
Pedro Hqs Medeiros
Author:
Solanka E. Ledwaba
Author:
Glynis L. Kolling
Author:
Kenneth Tung
Author:
Patricia Guerry
Author:
Richard L. Guerrant
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