Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters
Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters
Prebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host. Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli. This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites. Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments. In total, three different doses (1, 0·5 and 0·33 g equivalent to 0·1, 0·05 and 0·033 g/l) were tested. Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively. The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses. Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates. The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect. However, the applicability of such changes remains to be shown in an in vivo trial.
Acetic Acid/metabolism, Bacteria/drug effects, Bifidobacterium/drug effects, Butyric Acid/metabolism, Colon/metabolism, Fatty Acids, Volatile/metabolism, Feces, Fermentation, Galactose/pharmacology, Gastrointestinal Microbiome/drug effects, Humans, Lactobacillus/drug effects, Metabolomics, Oligosaccharides/pharmacology, Prebiotics, Propionates/metabolism
480-486
Grimaldi, Roberta
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Swann, Jonathan R.
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Vulevic, Jelena
135d2516-09f6-4c50-8c43-acf1b08c0cf1
Gibson, Glenn R.
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Costabile, Adele
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14 August 2016
Grimaldi, Roberta
0b4ed1f9-efab-4569-97a1-5d01e85749f8
Swann, Jonathan R.
7c11a66b-f4b8-4dbf-aa17-ad8b0561b85c
Vulevic, Jelena
135d2516-09f6-4c50-8c43-acf1b08c0cf1
Gibson, Glenn R.
24ac4753-4f78-475f-9766-5da179e2ab92
Costabile, Adele
2f54d3f0-8414-4844-8296-e97f6097e09e
Grimaldi, Roberta, Swann, Jonathan R., Vulevic, Jelena, Gibson, Glenn R. and Costabile, Adele
(2016)
Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters.
British Journal of Nutrition, 116 (3), .
(doi:10.1017/S0007114516002269).
Abstract
Prebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host. Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli. This study aimed to determine the in vitro potential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno® GOS (B-GOS) on gut microbiota composition and their metabolites. Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose-response anaerobic batch culture experiments. In total, three different doses (1, 0·5 and 0·33 g equivalent to 0·1, 0·05 and 0·033 g/l) were tested. Changes in the gut microbiota during a time course were identified by fluorescence in situ hybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by 1H-NMR analysis and GC, respectively. The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses. Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates. The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect. However, the applicability of such changes remains to be shown in an in vivo trial.
Text
fermentation properties and potential prebiotic activity of bimuno galactooligosaccharide 65 galactooligosaccharide content on in vitro gut microbiota parameters
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More information
Accepted/In Press date: 7 May 2016
e-pub ahead of print date: 8 June 2016
Published date: 14 August 2016
Keywords:
Acetic Acid/metabolism, Bacteria/drug effects, Bifidobacterium/drug effects, Butyric Acid/metabolism, Colon/metabolism, Fatty Acids, Volatile/metabolism, Feces, Fermentation, Galactose/pharmacology, Gastrointestinal Microbiome/drug effects, Humans, Lactobacillus/drug effects, Metabolomics, Oligosaccharides/pharmacology, Prebiotics, Propionates/metabolism
Identifiers
Local EPrints ID: 440824
URI: http://eprints.soton.ac.uk/id/eprint/440824
ISSN: 0007-1145
PURE UUID: a2b6b074-77b4-48e9-b089-0ea67af29a64
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Date deposited: 20 May 2020 16:30
Last modified: 17 Mar 2024 04:00
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Contributors
Author:
Roberta Grimaldi
Author:
Jelena Vulevic
Author:
Glenn R. Gibson
Author:
Adele Costabile
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