In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children
In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children
Children with autism spectrum disorders (ASD) often suffer gastrointestinal problems consistent with imbalances in the gut microbial population. Treatment with antibiotics or pro/prebiotics has been postulated to regulate microbiota and improve gut symptoms, but there is a lack of evidence for such approaches, especially for prebiotics. This study assessed the influence of a prebiotic galactooligosaccharide (B-GOS) on gut microbial ecology and metabolic function using faecal samples from autistic and non-autistic children in an in vitro gut model system. Bacteriology was analysed using flow cytometry combined with fluorescence in situ hybridization and metabolic activity by HPLC and 1H-NMR. Consistent with previous studies, the microbiota of children with ASD contained a higher number of Clostridium spp. and a lower number of bifidobacteria compared with non-autistic children. B-GOS administration significantly increased bifidobacterial populations in each compartment of the models, both with autistic and non-autistic-derived samples, and lactobacilli in the final vessel of non-autistic models. In addition, changes in other bacterial population have been seen in particular for Clostridium, Rosburia, Bacteroides, Atopobium, Faecalibacterium prausnitzii, Sutterella spp. and Veillonellaceae. Furthermore, the addition of B-GOS to the models significantly altered short-chain fatty acid production in both groups, and increased ethanol and lactate in autistic children.
Anti-Bacterial Agents, Autistic Disorder/microbiology, Bacteroides/metabolism, Bifidobacterium/growth & development, Child, Clostridium/metabolism, Fatty Acids, Volatile/metabolism, Feces/microbiology, Fermentation, Humans, In Situ Hybridization, Fluorescence, Lactic Acid, Lactobacillus/metabolism, Microbiota, Oligosaccharides/metabolism, Prebiotics
1-10
Grimaldi, Roberta
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Cela, Drinalda
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Swann, Jonathan R.
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Vulevic, Jelena
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Gibson, Glenn R.
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Tzortzis, George
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Costabile, Adele
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February 2017
Grimaldi, Roberta
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Cela, Drinalda
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Swann, Jonathan R.
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Vulevic, Jelena
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Gibson, Glenn R.
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Tzortzis, George
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Costabile, Adele
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Grimaldi, Roberta, Cela, Drinalda, Swann, Jonathan R., Vulevic, Jelena, Gibson, Glenn R., Tzortzis, George and Costabile, Adele
(2017)
In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children.
FEMS Microbiology Ecology, 93 (2), , [fiw233].
(doi:10.1093/femsec/fiw233).
Abstract
Children with autism spectrum disorders (ASD) often suffer gastrointestinal problems consistent with imbalances in the gut microbial population. Treatment with antibiotics or pro/prebiotics has been postulated to regulate microbiota and improve gut symptoms, but there is a lack of evidence for such approaches, especially for prebiotics. This study assessed the influence of a prebiotic galactooligosaccharide (B-GOS) on gut microbial ecology and metabolic function using faecal samples from autistic and non-autistic children in an in vitro gut model system. Bacteriology was analysed using flow cytometry combined with fluorescence in situ hybridization and metabolic activity by HPLC and 1H-NMR. Consistent with previous studies, the microbiota of children with ASD contained a higher number of Clostridium spp. and a lower number of bifidobacteria compared with non-autistic children. B-GOS administration significantly increased bifidobacterial populations in each compartment of the models, both with autistic and non-autistic-derived samples, and lactobacilli in the final vessel of non-autistic models. In addition, changes in other bacterial population have been seen in particular for Clostridium, Rosburia, Bacteroides, Atopobium, Faecalibacterium prausnitzii, Sutterella spp. and Veillonellaceae. Furthermore, the addition of B-GOS to the models significantly altered short-chain fatty acid production in both groups, and increased ethanol and lactate in autistic children.
Text
fiw233
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More information
Accepted/In Press date: 16 November 2016
e-pub ahead of print date: 16 November 2016
Published date: February 2017
Keywords:
Anti-Bacterial Agents, Autistic Disorder/microbiology, Bacteroides/metabolism, Bifidobacterium/growth & development, Child, Clostridium/metabolism, Fatty Acids, Volatile/metabolism, Feces/microbiology, Fermentation, Humans, In Situ Hybridization, Fluorescence, Lactic Acid, Lactobacillus/metabolism, Microbiota, Oligosaccharides/metabolism, Prebiotics
Identifiers
Local EPrints ID: 440837
URI: http://eprints.soton.ac.uk/id/eprint/440837
ISSN: 0168-6496
PURE UUID: aef6610c-2f47-48ba-b60a-883ea0d84353
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Date deposited: 20 May 2020 16:31
Last modified: 17 Mar 2024 04:00
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Contributors
Author:
Roberta Grimaldi
Author:
Drinalda Cela
Author:
Jelena Vulevic
Author:
Glenn R. Gibson
Author:
George Tzortzis
Author:
Adele Costabile
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