Docosahexaenoic acid and oleic acid induce altered DNA methylation of individual CpG loci in Jurkat T cells
Docosahexaenoic acid and oleic acid induce altered DNA methylation of individual CpG loci in Jurkat T cells
Docosahexaenoic acid (DHA, 22:6n-3) and oleic acid (18:1n-9) can alter the DNA methylation of individual CpG loci in vivo and in vitro, although the targeting mechanism is unknown. We tested the hypothesis that the targeting of altered methylation is associated with putative transcription factor response elements (pTREs) proximal to modified loci. Jurkat cells were treated with 22:6n-3 or 18:1n-9 (both 15 μM) for eight days and DNA methylation measured using the MethylationEPIC 850K array. 1596 CpG loci were altered significantly (508 hypermethylated) by 22:6n-3 and 563 CpG loci (294 hypermethylated) by 18:1n-9. 78 loci were modified by both fatty acids. Induced differential methylation was not modified by the PPARα antagonist GW6471. DNA sequences proximal to differentially methylated CpG loci were enriched in zinc-finger pTREs. These findings suggest that zinc-finger-containing transcription factors may be involved in targeting altered DNA methylation modifying processes induced by fatty acids to individual CpG loci.
DNA methylome,, Docosahexaenoic acid, Epigenetics, Jurkat cells, Oleic acid, Response element
1-10
Perez-Mojica, Jose Eduardo
a33c4e4e-9e33-4693-aa53-4a0298b5b10b
Lillycrop, Karen
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Burdge, Graham
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
July 2020
Perez-Mojica, Jose Eduardo
a33c4e4e-9e33-4693-aa53-4a0298b5b10b
Lillycrop, Karen
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Burdge, Graham
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Perez-Mojica, Jose Eduardo, Lillycrop, Karen, Cooper, Cyrus, Calder, Philip and Burdge, Graham
(2020)
Docosahexaenoic acid and oleic acid induce altered DNA methylation of individual CpG loci in Jurkat T cells.
Prostaglandins, Leukotrienes and Essential Fatty Acids, 158, , [102128].
(doi:10.1016/j.plefa.2020.102128).
Abstract
Docosahexaenoic acid (DHA, 22:6n-3) and oleic acid (18:1n-9) can alter the DNA methylation of individual CpG loci in vivo and in vitro, although the targeting mechanism is unknown. We tested the hypothesis that the targeting of altered methylation is associated with putative transcription factor response elements (pTREs) proximal to modified loci. Jurkat cells were treated with 22:6n-3 or 18:1n-9 (both 15 μM) for eight days and DNA methylation measured using the MethylationEPIC 850K array. 1596 CpG loci were altered significantly (508 hypermethylated) by 22:6n-3 and 563 CpG loci (294 hypermethylated) by 18:1n-9. 78 loci were modified by both fatty acids. Induced differential methylation was not modified by the PPARα antagonist GW6471. DNA sequences proximal to differentially methylated CpG loci were enriched in zinc-finger pTREs. These findings suggest that zinc-finger-containing transcription factors may be involved in targeting altered DNA methylation modifying processes induced by fatty acids to individual CpG loci.
Text
Methylation paper Accepted 7 May 2020
- Accepted Manuscript
More information
Accepted/In Press date: 7 May 2020
e-pub ahead of print date: 21 May 2020
Published date: July 2020
Additional Information:
Funding Information:
The study was funded by The Medical Research Council (UK) and The National Council for Science and Technology (CONACYT) (Mexico).
Funding Information:
GCB has received research funding from Nestle, Abbott Nutrition and Danone. He has served as member of the Scientific Advisory Board of BASF and is member of the BASF Asia Grant Panel. PCC acts as a consultant to BASF AS, Cargill, Smartfish, DSM and Fresenius-Kabi. The authors state they have no conflicts of interest related to the work presented in this manuscript. None of the other authors has disclosures or conflicts of interest to report.
Publisher Copyright:
© 2020 Elsevier Ltd
Keywords:
DNA methylome,, Docosahexaenoic acid, Epigenetics, Jurkat cells, Oleic acid, Response element
Identifiers
Local EPrints ID: 441016
URI: http://eprints.soton.ac.uk/id/eprint/441016
ISSN: 0952-3278
PURE UUID: bc16e606-56c6-45a1-a6d8-140748906143
Catalogue record
Date deposited: 27 May 2020 16:55
Last modified: 18 Mar 2024 05:10
Export record
Altmetrics
Contributors
Author:
Jose Eduardo Perez-Mojica
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
