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Docosahexaenoic acid and oleic acid induce altered DNA methylation of individual CpG loci in Jurkat T cells

Docosahexaenoic acid and oleic acid induce altered DNA methylation of individual CpG loci in Jurkat T cells
Docosahexaenoic acid and oleic acid induce altered DNA methylation of individual CpG loci in Jurkat T cells
Docosahexaenoic acid (DHA, 22:6n-3) and oleic acid (18:1n-9) can alter the DNA methylation of individual CpG loci in vivo and in vitro, although the targeting mechanism is unknown. We tested the hypothesis that the targeting of altered methylation is associated with putative transcription factor response elements (pTREs) proximal to modified loci. Jurkat cells were treated with 22:6n-3 or 18:1n-9 (both 15 μM) for eight days and DNA methylation measured using the MethylationEPIC 850K array. 1596 CpG loci were altered significantly (508 hypermethylated) by 22:6n-3 and 563 CpG loci (294 hypermethylated) by 18:1n-9. 78 loci were modified by both fatty acids. Induced differential methylation was not modified by the PPARα antagonist GW6471. DNA sequences proximal to differentially methylated CpG loci were enriched in zinc-finger pTREs. These findings suggest that zinc-finger-containing transcription factors may be involved in targeting altered DNA methylation modifying processes induced by fatty acids to individual CpG loci.
DNA methylome,, Docosahexaenoic acid, Epigenetics, Jurkat cells, Oleic acid, Response element
0952-3278
1-10
Perez-Mojica, Jose Eduardo
a33c4e4e-9e33-4693-aa53-4a0298b5b10b
Lillycrop, Karen
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Burdge, Graham
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Perez-Mojica, Jose Eduardo
a33c4e4e-9e33-4693-aa53-4a0298b5b10b
Lillycrop, Karen
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Burdge, Graham
09d60a07-8ca1-4351-9bf1-de6ffcfb2159

Perez-Mojica, Jose Eduardo, Lillycrop, Karen, Cooper, Cyrus, Calder, Philip and Burdge, Graham (2020) Docosahexaenoic acid and oleic acid induce altered DNA methylation of individual CpG loci in Jurkat T cells. Prostaglandins, Leukotrienes and Essential Fatty Acids, 158, 1-10, [102128]. (doi:10.1016/j.plefa.2020.102128).

Record type: Article

Abstract

Docosahexaenoic acid (DHA, 22:6n-3) and oleic acid (18:1n-9) can alter the DNA methylation of individual CpG loci in vivo and in vitro, although the targeting mechanism is unknown. We tested the hypothesis that the targeting of altered methylation is associated with putative transcription factor response elements (pTREs) proximal to modified loci. Jurkat cells were treated with 22:6n-3 or 18:1n-9 (both 15 μM) for eight days and DNA methylation measured using the MethylationEPIC 850K array. 1596 CpG loci were altered significantly (508 hypermethylated) by 22:6n-3 and 563 CpG loci (294 hypermethylated) by 18:1n-9. 78 loci were modified by both fatty acids. Induced differential methylation was not modified by the PPARα antagonist GW6471. DNA sequences proximal to differentially methylated CpG loci were enriched in zinc-finger pTREs. These findings suggest that zinc-finger-containing transcription factors may be involved in targeting altered DNA methylation modifying processes induced by fatty acids to individual CpG loci.

Text
Methylation paper Accepted 7 May 2020 - Accepted Manuscript
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More information

Accepted/In Press date: 7 May 2020
e-pub ahead of print date: 21 May 2020
Published date: July 2020
Keywords: DNA methylome,, Docosahexaenoic acid, Epigenetics, Jurkat cells, Oleic acid, Response element

Identifiers

Local EPrints ID: 441016
URI: http://eprints.soton.ac.uk/id/eprint/441016
ISSN: 0952-3278
PURE UUID: bc16e606-56c6-45a1-a6d8-140748906143
ORCID for Karen Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489
ORCID for Cyrus Cooper: ORCID iD orcid.org/0000-0003-3510-0709
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X
ORCID for Graham Burdge: ORCID iD orcid.org/0000-0002-7665-2967

Catalogue record

Date deposited: 27 May 2020 16:55
Last modified: 30 Apr 2021 04:01

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