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Spectroscopy on the wing: Investigating possible differences in protein secondary structures in feather shafts of birds using Raman spectroscopy

Spectroscopy on the wing: Investigating possible differences in protein secondary structures in feather shafts of birds using Raman spectroscopy
Spectroscopy on the wing: Investigating possible differences in protein secondary structures in feather shafts of birds using Raman spectroscopy
The central shaft of a bird’s flight feather bears most of the aerodynamic load during flight and exhibits some remarkable mechanical properties. The shaft comprises two parts, the calamus and the rachis. The calamus is at the base of the shaft, while the rachis is the longer upper part which supports the vanes. The shaft is composed of a fibrous outer cortex, and an inner foam-like core. Recent nanoindentation experiments have indicated that reduced modulus values, Er, for the inner and outer regions of the cortex can vary, with the Er values of the inner region slightly greater than those of the outer region.
In this work, Raman spectroscopy is used to investigate the protein secondary structures in the inner and outer regions of the feather cortex. Analysis of the Amide Iregion of Raman spectra taken from four birds (Swan, Gull, Mallard and Kestrel) shows that the β-sheet structural component decreases between the inner and outer region, relative to the protein side-chain components. This finding is consistent with the proposal that Er values are greater in the inner region than the outer region.
This work has shown that Raman spectroscopy can be used effectively to study the change in protein secondary structure between the inner and outer regions of a feather shaft.
Feather keratin, Feather shaft, Feathers, Protein secondary structure, Raman spectroscopy
1047-8477
Laurent, Christian
fdcd3853-e6d3-47a8-afed-650ae70f65d9
Dyke, John
46393b45-6694-46f3-af20-d7369d26199f
Cook, Richard
06f8322d-81be-4f82-9326-19e55541c78f
Dyke, Gareth
3bc72760-3e83-4bc8-808d-2adff0a09b6d
De Kat, Roeland
d46a99a4-8653-4698-9ef4-46dd0c77ba5d
Laurent, Christian
fdcd3853-e6d3-47a8-afed-650ae70f65d9
Dyke, John
46393b45-6694-46f3-af20-d7369d26199f
Cook, Richard
06f8322d-81be-4f82-9326-19e55541c78f
Dyke, Gareth
3bc72760-3e83-4bc8-808d-2adff0a09b6d
De Kat, Roeland
d46a99a4-8653-4698-9ef4-46dd0c77ba5d

Laurent, Christian, Dyke, John, Cook, Richard, Dyke, Gareth and De Kat, Roeland (2020) Spectroscopy on the wing: Investigating possible differences in protein secondary structures in feather shafts of birds using Raman spectroscopy. Journal of Structural Biology, 211 (1), [107529]. (doi:10.1016/j.jsb.2020.107529).

Record type: Article

Abstract

The central shaft of a bird’s flight feather bears most of the aerodynamic load during flight and exhibits some remarkable mechanical properties. The shaft comprises two parts, the calamus and the rachis. The calamus is at the base of the shaft, while the rachis is the longer upper part which supports the vanes. The shaft is composed of a fibrous outer cortex, and an inner foam-like core. Recent nanoindentation experiments have indicated that reduced modulus values, Er, for the inner and outer regions of the cortex can vary, with the Er values of the inner region slightly greater than those of the outer region.
In this work, Raman spectroscopy is used to investigate the protein secondary structures in the inner and outer regions of the feather cortex. Analysis of the Amide Iregion of Raman spectra taken from four birds (Swan, Gull, Mallard and Kestrel) shows that the β-sheet structural component decreases between the inner and outer region, relative to the protein side-chain components. This finding is consistent with the proposal that Er values are greater in the inner region than the outer region.
This work has shown that Raman spectroscopy can be used effectively to study the change in protein secondary structure between the inner and outer regions of a feather shaft.

Text
MASTERFeatherRamanPaper - Accepted Manuscript
Restricted to Repository staff only until 19 May 2021.
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More information

Accepted/In Press date: 3 May 2020
e-pub ahead of print date: 19 May 2020
Published date: 1 July 2020
Keywords: Feather keratin, Feather shaft, Feathers, Protein secondary structure, Raman spectroscopy

Identifiers

Local EPrints ID: 441045
URI: http://eprints.soton.ac.uk/id/eprint/441045
ISSN: 1047-8477
PURE UUID: 07f18e77-6639-41f9-8191-862f3c9d66da
ORCID for Christian Laurent: ORCID iD orcid.org/0000-0002-2946-9705
ORCID for John Dyke: ORCID iD orcid.org/0000-0002-9808-303X
ORCID for Roeland De Kat: ORCID iD orcid.org/0000-0002-6851-4409

Catalogue record

Date deposited: 28 May 2020 16:57
Last modified: 18 Feb 2021 17:27

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