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Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform

Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform
Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform
Tuberculosis (TB) remains a leading cause of death worldwide. Lipid
rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics
and may be responsible for relapse and the need for long-term TB treatment.
We present a microfluidic system that acoustically traps live mycobacteria,
M. smegmatis, a model organism for M. tuberculosis. We then perform
optical analysis in the form of wavelength modulated Raman spectroscopy
(WMRS) on the trapped M. smegmatis for up to eight hours, and also in
the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis
exposed to INH change substantially in comparison to the unstressed condition.
Our work provides a real-time assessment of the impact of INH on the
increase of lipids in these mycobacteria, which could render the cells more
tolerant to antibiotics. This microfluidic platform may be used to study any
microorganism and to dynamically monitor its response to different conditions
and stimuli.
Real-time monitoring, live mycobacteria, microfluidic acoustic-Raman platform
2399-3642
1-8
Baron, Vincent O.
2cb84933-b07a-4d1a-aa20-eebdcb93c822
Chen, Mingzhou
79a02a48-4960-4670-af3e-4a0004364235
Hammarstrom, Bjorn
c1f33fd2-4031-4ec7-b36a-ec3aff48c164
Hammond, Robert J. H.
ee25b7b3-4cbd-44f6-836f-7b201392fda1
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Gillespie, Stephen H.
cce81e04-6067-4050-a284-9830ad6bd9a9
Dholakia, Kishan
c649b0a9-ab68-4571-b8c7-e84553d14174
Baron, Vincent O.
2cb84933-b07a-4d1a-aa20-eebdcb93c822
Chen, Mingzhou
79a02a48-4960-4670-af3e-4a0004364235
Hammarstrom, Bjorn
c1f33fd2-4031-4ec7-b36a-ec3aff48c164
Hammond, Robert J. H.
ee25b7b3-4cbd-44f6-836f-7b201392fda1
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Gillespie, Stephen H.
cce81e04-6067-4050-a284-9830ad6bd9a9
Dholakia, Kishan
c649b0a9-ab68-4571-b8c7-e84553d14174

Baron, Vincent O., Chen, Mingzhou, Hammarstrom, Bjorn, Hammond, Robert J. H., Glynne-Jones, Peter, Gillespie, Stephen H. and Dholakia, Kishan (2020) Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform. Communications Biology, 3 (1), 1-8, [236]. (doi:10.1038/s42003-020-0915-3).

Record type: Article

Abstract

Tuberculosis (TB) remains a leading cause of death worldwide. Lipid
rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics
and may be responsible for relapse and the need for long-term TB treatment.
We present a microfluidic system that acoustically traps live mycobacteria,
M. smegmatis, a model organism for M. tuberculosis. We then perform
optical analysis in the form of wavelength modulated Raman spectroscopy
(WMRS) on the trapped M. smegmatis for up to eight hours, and also in
the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis
exposed to INH change substantially in comparison to the unstressed condition.
Our work provides a real-time assessment of the impact of INH on the
increase of lipids in these mycobacteria, which could render the cells more
tolerant to antibiotics. This microfluidic platform may be used to study any
microorganism and to dynamically monitor its response to different conditions
and stimuli.

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More information

Accepted/In Press date: 26 March 2020
e-pub ahead of print date: 14 May 2020
Published date: 14 May 2020
Keywords: Real-time monitoring, live mycobacteria, microfluidic acoustic-Raman platform

Identifiers

Local EPrints ID: 441063
URI: http://eprints.soton.ac.uk/id/eprint/441063
ISSN: 2399-3642
PURE UUID: 40399c78-a6ec-40c9-b712-1cb28534b344
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953

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Date deposited: 28 May 2020 16:58
Last modified: 28 Apr 2022 01:46

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Contributors

Author: Vincent O. Baron
Author: Mingzhou Chen
Author: Bjorn Hammarstrom
Author: Robert J. H. Hammond
Author: Stephen H. Gillespie
Author: Kishan Dholakia

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