The University of Southampton
University of Southampton Institutional Repository

Maternal protein restriction around conception reduces the neural stem cells during mouse fetal brain development and alters neuronal differentiation during gestation

Maternal protein restriction around conception reduces the neural stem cells during mouse fetal brain development and alters neuronal differentiation during gestation
Maternal protein restriction around conception reduces the neural stem cells during mouse fetal brain development and alters neuronal differentiation during gestation
Introduction: maternal malnutrition during pregnancy is detrimental to fetal development and increases the risk of many chronic diseases in later life i.e. neurological consequences such as increased risk of schizophrenia. Previous studies have shown maternal protein malnutrition during pregnancy and lactation compromises brain development in late gestation and after birth, affecting structural, biochemical and pathway dynamics with lasting consequences for motor and cognitive function. However, the importance of nutrition during embryogenesis for early brain development is unknown. We have previously shown maternal low protein diet confined to the preimplantation period (Emb-LPD) in mice is sufficient to induce cardiometabolic and locomotory behavioural abnormalities in adult offspring.Methods: using a diet model, female mice were fed different diets from conception to the end of pregnancy: normal protein diet (NPD), low protein diet (LPD) or embryonic LPD (Emb-LPD: LPD for 3.5 days, NPD thereafter). Fetal brains were analysed at three time points in gestation (E12.5, E14.5 & E17.5), with in vivo analysis using FACS and immunofluorescence for neural stem cell and neuron markers, and in vitro techniques using the neurosphere culture assay. We have also carried out a number of follow up behavioural tests for memory including novel object recognition in adult offspring.Results & discussion: we have shown that Emb-LPD and sustained LPD reduce neural stem cell (NSC) and progenitor cell numbers through suppressed proliferation rates in both ganglionic eminences and cortex of the fetal brain at E12.5, E14.5 & E17.5. Moreover, Emb-LPD causes remaining NSCs to upregulate the neuronal differentiation rate in compensation beyond control levels. We have also seen a deficit in short term memory in the Emb-LPD adult offspring. This data is the first to clearly demonstrate that poor maternal nutrition around conception has adverse effects on early brain development, which might be associated with the adult behavioural deficits.
2052-1472
Gould, Joanna
96c504c1-273b-4104-98e6-87931fe763bf
Smith, Phoebe J.
0514b7c4-d671-4b68-8a95-9b5afddab988
Airey, Chris J.
92a7831b-b617-42e0-8ebd-bd794ecfd21c
Warricker, Frazer D.M.
57296e2b-40de-43ce-bf95-007af1bae9e7
Restall, Katie
b6f45023-84bf-4d48-87f9-6f202379ab55
Egar, J
c8183ad9-6236-47b3-9acd-7487ac0f2c01
Marfy-smith, Stephanie J
ee107f53-c871-4a11-b494-dd185cdf5f1e
Dewing, Jennifer
868fbf01-7b6e-499f-abf9-47409278373f
Fleming, Tom
86f3e49b-afaf-4b64-9473-03a8ad9436cf
Willaime-Morawek, Sandrine
24a2981f-aa9e-4bf6-ad12-2ccf6b49f1c0
Gould, Joanna
96c504c1-273b-4104-98e6-87931fe763bf
Smith, Phoebe J.
0514b7c4-d671-4b68-8a95-9b5afddab988
Airey, Chris J.
92a7831b-b617-42e0-8ebd-bd794ecfd21c
Warricker, Frazer D.M.
57296e2b-40de-43ce-bf95-007af1bae9e7
Restall, Katie
b6f45023-84bf-4d48-87f9-6f202379ab55
Egar, J
c8183ad9-6236-47b3-9acd-7487ac0f2c01
Marfy-smith, Stephanie J
ee107f53-c871-4a11-b494-dd185cdf5f1e
Dewing, Jennifer
868fbf01-7b6e-499f-abf9-47409278373f
Fleming, Tom
86f3e49b-afaf-4b64-9473-03a8ad9436cf
Willaime-Morawek, Sandrine
24a2981f-aa9e-4bf6-ad12-2ccf6b49f1c0

Gould, Joanna, Smith, Phoebe J., Airey, Chris J., Warricker, Frazer D.M., Restall, Katie, Egar, J, Marfy-smith, Stephanie J, Dewing, Jennifer, Fleming, Tom and Willaime-Morawek, Sandrine (2016) Maternal protein restriction around conception reduces the neural stem cells during mouse fetal brain development and alters neuronal differentiation during gestation. Reproduction Abstracts. (doi:10.1530/repabs.3.O024).

Record type: Meeting abstract

Abstract

Introduction: maternal malnutrition during pregnancy is detrimental to fetal development and increases the risk of many chronic diseases in later life i.e. neurological consequences such as increased risk of schizophrenia. Previous studies have shown maternal protein malnutrition during pregnancy and lactation compromises brain development in late gestation and after birth, affecting structural, biochemical and pathway dynamics with lasting consequences for motor and cognitive function. However, the importance of nutrition during embryogenesis for early brain development is unknown. We have previously shown maternal low protein diet confined to the preimplantation period (Emb-LPD) in mice is sufficient to induce cardiometabolic and locomotory behavioural abnormalities in adult offspring.Methods: using a diet model, female mice were fed different diets from conception to the end of pregnancy: normal protein diet (NPD), low protein diet (LPD) or embryonic LPD (Emb-LPD: LPD for 3.5 days, NPD thereafter). Fetal brains were analysed at three time points in gestation (E12.5, E14.5 & E17.5), with in vivo analysis using FACS and immunofluorescence for neural stem cell and neuron markers, and in vitro techniques using the neurosphere culture assay. We have also carried out a number of follow up behavioural tests for memory including novel object recognition in adult offspring.Results & discussion: we have shown that Emb-LPD and sustained LPD reduce neural stem cell (NSC) and progenitor cell numbers through suppressed proliferation rates in both ganglionic eminences and cortex of the fetal brain at E12.5, E14.5 & E17.5. Moreover, Emb-LPD causes remaining NSCs to upregulate the neuronal differentiation rate in compensation beyond control levels. We have also seen a deficit in short term memory in the Emb-LPD adult offspring. This data is the first to clearly demonstrate that poor maternal nutrition around conception has adverse effects on early brain development, which might be associated with the adult behavioural deficits.

Full text not available from this repository.

More information

Published date: 2016
Venue - Dates: Society For Reproduction 2016, United Kingdom, 2016-07-10 - 2016-07-13

Identifiers

Local EPrints ID: 441117
URI: http://eprints.soton.ac.uk/id/eprint/441117
ISSN: 2052-1472
PURE UUID: faab64fe-ee41-4b14-ad55-b50b752eb091
ORCID for Sandrine Willaime-Morawek: ORCID iD orcid.org/0000-0002-1121-6419

Catalogue record

Date deposited: 02 Jun 2020 16:30
Last modified: 18 Feb 2021 17:11

Export record

Altmetrics

Contributors

Author: Joanna Gould
Author: Phoebe J. Smith
Author: Chris J. Airey
Author: Frazer D.M. Warricker
Author: Katie Restall
Author: J Egar
Author: Stephanie J Marfy-smith
Author: Jennifer Dewing
Author: Tom Fleming

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×