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Microstreaming inside model cells induced by ultrasound and microbubbles

Microstreaming inside model cells induced by ultrasound and microbubbles
Microstreaming inside model cells induced by ultrasound and microbubbles
Studies on the bioeffects produced by ultrasound and microbubbles have focused primarily on transport in bulk tissue, drug uptake by individual cells, and disruption of biological membranes. Relatively little is known about the physical perturbations and fluid dynamics of the intracellular environment during ultrasound exposure. To investigate this, a custom acoustofluidic chamber was designed to expose model cells, in the form of giant unilamellar vesicles, to ultrasound and microbubbles. The motion of fluorescent tracer beads within the lumen of the vesicles was tracked during exposure to laminar flow (~1mm s-1), ultrasound (1MHz, ~150kPa, 60s), and phospholipid coated microbubbles, alone and in combination. To decouple the effects of fluid flow and ultrasound exposure, the system was also modelled numerically using boundary-driven streaming field equations. Both the experimental and numerical results indicate that all conditions produced internal streaming within the vesicles. Ultrasound alone produced an average bead velocity of 6.5±1.3 µm/s, which increased to 8.5±3.8 µm/s in the presence of microbubbles compared to 12±0.12 µm/s under laminar flow. Further research on intracellular forces in mammalian cells and the associated biological effects in vitro and in vivo are required to fully determine the implications for safety and/or therapy.
0743-7463
1-40
Pereno, Valerio
3106035d-e7d9-4af8-af59-dd5091532ea7
Lei, Junjun
7fb90120-6906-4ed2-9e12-7d0d17fffcd6
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
Pereno, Valerio
3106035d-e7d9-4af8-af59-dd5091532ea7
Lei, Junjun
7fb90120-6906-4ed2-9e12-7d0d17fffcd6
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6

Pereno, Valerio, Lei, Junjun, Carugo, Dario and Stride, Eleanor (2020) Microstreaming inside model cells induced by ultrasound and microbubbles. Langmuir, 36 (23), 1-40. (doi:10.1021/acs.langmuir.0c00536).

Record type: Article

Abstract

Studies on the bioeffects produced by ultrasound and microbubbles have focused primarily on transport in bulk tissue, drug uptake by individual cells, and disruption of biological membranes. Relatively little is known about the physical perturbations and fluid dynamics of the intracellular environment during ultrasound exposure. To investigate this, a custom acoustofluidic chamber was designed to expose model cells, in the form of giant unilamellar vesicles, to ultrasound and microbubbles. The motion of fluorescent tracer beads within the lumen of the vesicles was tracked during exposure to laminar flow (~1mm s-1), ultrasound (1MHz, ~150kPa, 60s), and phospholipid coated microbubbles, alone and in combination. To decouple the effects of fluid flow and ultrasound exposure, the system was also modelled numerically using boundary-driven streaming field equations. Both the experimental and numerical results indicate that all conditions produced internal streaming within the vesicles. Ultrasound alone produced an average bead velocity of 6.5±1.3 µm/s, which increased to 8.5±3.8 µm/s in the presence of microbubbles compared to 12±0.12 µm/s under laminar flow. Further research on intracellular forces in mammalian cells and the associated biological effects in vitro and in vivo are required to fully determine the implications for safety and/or therapy.

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Accepted/In Press date: 14 May 2020
e-pub ahead of print date: 14 May 2020

Identifiers

Local EPrints ID: 441143
URI: http://eprints.soton.ac.uk/id/eprint/441143
ISSN: 0743-7463
PURE UUID: e49f4e81-e124-407e-891d-56fe9ce0f35e

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Date deposited: 03 Jun 2020 16:30
Last modified: 26 Nov 2021 06:15

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Contributors

Author: Valerio Pereno
Author: Junjun Lei
Author: Dario Carugo
Author: Eleanor Stride

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