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Maternal High Fat Diet (HFD) in the adult offspring brain modifies cell density and neuronal density

Maternal High Fat Diet (HFD) in the adult offspring brain modifies cell density and neuronal density
Maternal High Fat Diet (HFD) in the adult offspring brain modifies cell density and neuronal density
Introduction: an upward trend in maternal obesity is rising every year. Different data suggest that maternal obesity during gestation may have effects on a high risk of children of developing physiological and psychological dysfunctions in later life. Animal models suggest that a maternal high fat diet (HFD) during pregnancy could have enduring consequences on brain structure and development in the offspring. Our aim is to evaluate the effects of maternal HFD on both offspring brain development and neural stems cells (NSCs).
Methods: female mice were fed different diets from conception: chow diet (CD), HFD throughout gestation and lactation (HFD) or embryonic HFD (Emb-HFD: HFD for 3.5 days, CD thereafter). After weaning, the offspring were maintained on CD. 5 male brains and 6 female brains were collected per group and analysed by immunostaining.
Results and discussion: we showed an increase in cortical layer thickness (layer 2/3 P=0.0461 layer 6 P=0.0023) increase in total cortical cell density (layer 2/3 P=0.0087 layer 5 P=0.0266 layer 6 P=0.0080) and reduction in neuronal proportion (layer 2/3 P=0.0601 layer 4 P=0.0025 layer 6 P=0.0039) in the HFD males compared with CD males. Similar results have been found in female offspring brains, with increase cell density (layer 4 P=0.0058, layer 5 P=0.0010) in the HFD females compared with CD females. Additionally, when NSCs were examined in the subventricular zone, Emb-HFD males showed increased neural stem cells compared to CD males (P<0.05). However, there was no significant difference in the density of astrocytes or microglia between male groups. Further work will determine the cells responsible for the increase cell density. Taken together, our data suggests that neurogenesis and brain morphology are altered following maternal HFD and this might result in long term changes in brain architecture. Further research will be important for a better understanding of the effect of maternal HFD on brain development.
2052-1472
Ojeda Pedraza, Diego, Armando
967ac799-26ba-4589-b507-4a88a1ca1859
Jane-Coupe, K
3cc3d42e-3d6d-45ec-b7d5-db76a92c01cd
Earl, Megan
dc2b5afc-7ec0-49a2-ae61-44a7b9c52b6e
Hutton, Oliver
2127307e-5e63-45ed-82de-04238018c11b
Eckert, Judith
729bfa49-7053-458d-8e84-3e70e4d98e57
Willaime-Morawek, Sandrine
24a2981f-aa9e-4bf6-ad12-2ccf6b49f1c0
Ojeda Pedraza, Diego, Armando
967ac799-26ba-4589-b507-4a88a1ca1859
Jane-Coupe, K
3cc3d42e-3d6d-45ec-b7d5-db76a92c01cd
Earl, Megan
dc2b5afc-7ec0-49a2-ae61-44a7b9c52b6e
Hutton, Oliver
2127307e-5e63-45ed-82de-04238018c11b
Eckert, Judith
729bfa49-7053-458d-8e84-3e70e4d98e57
Willaime-Morawek, Sandrine
24a2981f-aa9e-4bf6-ad12-2ccf6b49f1c0

Ojeda Pedraza, Diego, Armando, Jane-Coupe, K, Earl, Megan, Hutton, Oliver, Eckert, Judith and Willaime-Morawek, Sandrine (2016) Maternal High Fat Diet (HFD) in the adult offspring brain modifies cell density and neuronal density. Reproduction Abstracts, 3. (doi:10.1530/repabs.3.O020).

Record type: Meeting abstract

Abstract

Introduction: an upward trend in maternal obesity is rising every year. Different data suggest that maternal obesity during gestation may have effects on a high risk of children of developing physiological and psychological dysfunctions in later life. Animal models suggest that a maternal high fat diet (HFD) during pregnancy could have enduring consequences on brain structure and development in the offspring. Our aim is to evaluate the effects of maternal HFD on both offspring brain development and neural stems cells (NSCs).
Methods: female mice were fed different diets from conception: chow diet (CD), HFD throughout gestation and lactation (HFD) or embryonic HFD (Emb-HFD: HFD for 3.5 days, CD thereafter). After weaning, the offspring were maintained on CD. 5 male brains and 6 female brains were collected per group and analysed by immunostaining.
Results and discussion: we showed an increase in cortical layer thickness (layer 2/3 P=0.0461 layer 6 P=0.0023) increase in total cortical cell density (layer 2/3 P=0.0087 layer 5 P=0.0266 layer 6 P=0.0080) and reduction in neuronal proportion (layer 2/3 P=0.0601 layer 4 P=0.0025 layer 6 P=0.0039) in the HFD males compared with CD males. Similar results have been found in female offspring brains, with increase cell density (layer 4 P=0.0058, layer 5 P=0.0010) in the HFD females compared with CD females. Additionally, when NSCs were examined in the subventricular zone, Emb-HFD males showed increased neural stem cells compared to CD males (P<0.05). However, there was no significant difference in the density of astrocytes or microglia between male groups. Further work will determine the cells responsible for the increase cell density. Taken together, our data suggests that neurogenesis and brain morphology are altered following maternal HFD and this might result in long term changes in brain architecture. Further research will be important for a better understanding of the effect of maternal HFD on brain development.

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More information

Published date: 10 July 2016
Venue - Dates: Society For Reproduction 2016, United Kingdom, 2016-07-10 - 2016-07-13

Identifiers

Local EPrints ID: 441164
URI: http://eprints.soton.ac.uk/id/eprint/441164
ISSN: 2052-1472
PURE UUID: 736acab3-455a-476b-ac1b-0ec0d03e2672
ORCID for Sandrine Willaime-Morawek: ORCID iD orcid.org/0000-0002-1121-6419

Catalogue record

Date deposited: 03 Jun 2020 16:31
Last modified: 18 Feb 2021 17:11

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Contributors

Author: Diego, Armando Ojeda Pedraza
Author: K Jane-Coupe
Author: Megan Earl
Author: Oliver Hutton
Author: Judith Eckert

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