The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Maternal protein restriction around conception alters the fetal mouse brain by reducing the neural stem cells and increasing neuronal differentiation during gestation, which is associated with the adult offspring behavioural deficits.

Maternal protein restriction around conception alters the fetal mouse brain by reducing the neural stem cells and increasing neuronal differentiation during gestation, which is associated with the adult offspring behavioural deficits.
Maternal protein restriction around conception alters the fetal mouse brain by reducing the neural stem cells and increasing neuronal differentiation during gestation, which is associated with the adult offspring behavioural deficits.
Maternal malnutrition during pregnancy is detrimental to fetal development and increases the risk of many chronic diseases in later life i.e. neurological consequences such as increased risk of schizophrenia. Previous studies have shown maternal protein malnutrition during pregnancy and lactation compromises brain development in late gestation and after birth, affecting structural, biochemical and pathway dynamics with lasting consequences for motor and cognitive function. However, the importance of nutrition during embryogenesis for early brain development is unknown. We have previously shown maternal low protein diet confined to the preimplantation period (Emb-LPD) in mice is sufficient to induce cardiometabolic and behavioural abnormalities in adult offspring. Using a diet model, female mice were fed different diets from conception to the end of pregnancy: normal protein diet (NPD), low protein diet (LPD) or embryonic LPD (Emb-LPD: LPD for 3.5 days, NPD thereafter). Fetal brains were analysed at three timepoints in gestation (E12.5, E14.5 & E17.5), with in vivo analysisfor neural stem cell and neuron markers, and in vitro techniques using the neurosphere culture assay. We have also carried out a number of follow up behavioural tests, including short term novel object recognition test in adult offspring. We have shown that Emb-LPD and sustained LPD reduce neural stem cell (NSC) and progenitor cell numbers through suppressed proliferation rates in both ganglionic eminences and cortex of the fetal brain at E14.5 & E17.5(p=0.05). Moreover, Emb-LPD causes remaining NSCs to upregulate the neuronal differentiation rate in compensation beyond control levels (p=0.01). We have also seen a significant deficit in short term memory in the Emb-LPD adult offspring (p=0.0001). This study is the first to clearly demonstrate that poor maternal nutrition around conception has advers effects on early brain development & the adult offspring behavioural deficits.
University of Milan
Gould, Joanna
96c504c1-273b-4104-98e6-87931fe763bf
Pearson-Farr, Jennifer, Elizabeth
e7da2e7c-e8e8-47d6-aca4-86b1a5b90653
Airey, Lauren E
57265993-8217-4bad-9f92-92d2de0aa317
Smith, Phoebe J.
0514b7c4-d671-4b68-8a95-9b5afddab988
Fleming, Tom
86f3e49b-afaf-4b64-9473-03a8ad9436cf
Willaime-Morawek, Sandrine
24a2981f-aa9e-4bf6-ad12-2ccf6b49f1c0
Gould, Joanna
96c504c1-273b-4104-98e6-87931fe763bf
Pearson-Farr, Jennifer, Elizabeth
e7da2e7c-e8e8-47d6-aca4-86b1a5b90653
Airey, Lauren E
57265993-8217-4bad-9f92-92d2de0aa317
Smith, Phoebe J.
0514b7c4-d671-4b68-8a95-9b5afddab988
Fleming, Tom
86f3e49b-afaf-4b64-9473-03a8ad9436cf
Willaime-Morawek, Sandrine
24a2981f-aa9e-4bf6-ad12-2ccf6b49f1c0

Gould, Joanna, Pearson-Farr, Jennifer, Elizabeth, Airey, Lauren E, Smith, Phoebe J., Fleming, Tom and Willaime-Morawek, Sandrine (2016) Maternal protein restriction around conception alters the fetal mouse brain by reducing the neural stem cells and increasing neuronal differentiation during gestation, which is associated with the adult offspring behavioural deficits. In Epigenetics and Periconception Environment-Proceedings of the EPICONCEPT Conference 2016. University of Milan..

Record type: Conference or Workshop Item (Paper)

Abstract

Maternal malnutrition during pregnancy is detrimental to fetal development and increases the risk of many chronic diseases in later life i.e. neurological consequences such as increased risk of schizophrenia. Previous studies have shown maternal protein malnutrition during pregnancy and lactation compromises brain development in late gestation and after birth, affecting structural, biochemical and pathway dynamics with lasting consequences for motor and cognitive function. However, the importance of nutrition during embryogenesis for early brain development is unknown. We have previously shown maternal low protein diet confined to the preimplantation period (Emb-LPD) in mice is sufficient to induce cardiometabolic and behavioural abnormalities in adult offspring. Using a diet model, female mice were fed different diets from conception to the end of pregnancy: normal protein diet (NPD), low protein diet (LPD) or embryonic LPD (Emb-LPD: LPD for 3.5 days, NPD thereafter). Fetal brains were analysed at three timepoints in gestation (E12.5, E14.5 & E17.5), with in vivo analysisfor neural stem cell and neuron markers, and in vitro techniques using the neurosphere culture assay. We have also carried out a number of follow up behavioural tests, including short term novel object recognition test in adult offspring. We have shown that Emb-LPD and sustained LPD reduce neural stem cell (NSC) and progenitor cell numbers through suppressed proliferation rates in both ganglionic eminences and cortex of the fetal brain at E14.5 & E17.5(p=0.05). Moreover, Emb-LPD causes remaining NSCs to upregulate the neuronal differentiation rate in compensation beyond control levels (p=0.01). We have also seen a significant deficit in short term memory in the Emb-LPD adult offspring (p=0.0001). This study is the first to clearly demonstrate that poor maternal nutrition around conception has advers effects on early brain development & the adult offspring behavioural deficits.

This record has no associated files available for download.

More information

Published date: 26 September 2016
Venue - Dates: Epiconcept Conference 2016, Giardini Naxos, Sicily, Italy, 2016-09-26 - 2016-09-29

Identifiers

Local EPrints ID: 441165
URI: http://eprints.soton.ac.uk/id/eprint/441165
PURE UUID: 469be3ff-0be3-496d-99e5-85001fc8cecc
ORCID for Sandrine Willaime-Morawek: ORCID iD orcid.org/0000-0002-1121-6419

Catalogue record

Date deposited: 03 Jun 2020 16:31
Last modified: 18 Feb 2021 17:11

Export record

Contributors

Author: Joanna Gould
Author: Jennifer, Elizabeth Pearson-Farr
Author: Lauren E Airey
Author: Phoebe J. Smith
Author: Tom Fleming

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×