Magnetically aligned membrane mimetics enabling comparable chiroptical and magnetic resonance spectroscopy studies
Magnetically aligned membrane mimetics enabling comparable chiroptical and magnetic resonance spectroscopy studies
Lipidic bicelles have been widely used for the analysis of integral membrane proteins where their spontaneous alignment in an magnetic field has been exploited for oriented sample solid-state NMR studies. Many of their physical properties however make them well suited to the analysis of membrane proteins by circular dichroism (CD) and synchrotron radiation circular dichroism (SRCD). In this paper we have identified bicelle compositions that permit comparable studies of integral membrane proteins by solid-state NMR, CD, and SRCD; complementary methods that can provide insights into protein structure and the interactions with drugs and other small molecules. Furthermore we have been able to identify bicelle compositions that allow the magnetic alignment of the bicelles at fields routinely available in magnetic CD instruments. This potentially provides a route to the preparation of samples for oriented CD that mitigates many of the issues associated with the preparation of mechanically aligned samples, although we demonstrate that the dynamics present within the system can complicate the analysis of such spectra.
Bicelles, Cell membranes, Circular Dichroism, Magnetically alignable lipid phases, NMR, Synchrotron radiation circular dichroism
Evans, Luke S.
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Hussain, Rohanah
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Siligardi, Giuliano
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Williamson, Philip T.F.
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a
1 September 2020
Evans, Luke S.
c888a996-eb12-4e6b-9c2b-e6cda907674a
Hussain, Rohanah
d46302e5-4734-4f59-9ac1-bc7b1f62693c
Siligardi, Giuliano
ebce490a-2faa-4b98-9703-ae0d6f8e46c2
Williamson, Philip T.F.
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a
Evans, Luke S., Hussain, Rohanah, Siligardi, Giuliano and Williamson, Philip T.F.
(2020)
Magnetically aligned membrane mimetics enabling comparable chiroptical and magnetic resonance spectroscopy studies.
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1862 (9), [183343].
(doi:10.1016/j.bbamem.2020.183343).
Abstract
Lipidic bicelles have been widely used for the analysis of integral membrane proteins where their spontaneous alignment in an magnetic field has been exploited for oriented sample solid-state NMR studies. Many of their physical properties however make them well suited to the analysis of membrane proteins by circular dichroism (CD) and synchrotron radiation circular dichroism (SRCD). In this paper we have identified bicelle compositions that permit comparable studies of integral membrane proteins by solid-state NMR, CD, and SRCD; complementary methods that can provide insights into protein structure and the interactions with drugs and other small molecules. Furthermore we have been able to identify bicelle compositions that allow the magnetic alignment of the bicelles at fields routinely available in magnetic CD instruments. This potentially provides a route to the preparation of samples for oriented CD that mitigates many of the issues associated with the preparation of mechanically aligned samples, although we demonstrate that the dynamics present within the system can complicate the analysis of such spectra.
Text
Magnetically aligned membrane mimetics
- Accepted Manuscript
More information
Accepted/In Press date: 4 May 2020
e-pub ahead of print date: 14 May 2020
Published date: 1 September 2020
Additional Information:
Funding Information:
LSE was the recipient of PhD funding from Diamond Light Source and the Centre for Biological Science, University of Southampton. Studies have been conducted on the B23 Beamline at Diamond under time allocations SM12844, SM14084 and SM16501. M
Publisher Copyright:
© 2020 Elsevier B.V.
Keywords:
Bicelles, Cell membranes, Circular Dichroism, Magnetically alignable lipid phases, NMR, Synchrotron radiation circular dichroism
Identifiers
Local EPrints ID: 441182
URI: http://eprints.soton.ac.uk/id/eprint/441182
ISSN: 0005-2736
PURE UUID: 77ed7228-7f4b-4e00-92fd-abeb1b2585e1
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Date deposited: 04 Jun 2020 16:30
Last modified: 17 Mar 2024 05:36
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Contributors
Author:
Luke S. Evans
Author:
Rohanah Hussain
Author:
Giuliano Siligardi
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