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Magnetically aligned membrane mimetics enabling comparable chiroptical and magnetic resonance spectroscopy studies

Magnetically aligned membrane mimetics enabling comparable chiroptical and magnetic resonance spectroscopy studies
Magnetically aligned membrane mimetics enabling comparable chiroptical and magnetic resonance spectroscopy studies
Lipidic bicelles have been widely used for the analysis of integral membrane proteins where their spontaneous alignment in an magnetic field has been exploited for oriented sample solid-state NMR studies. Many of their physical properties however make them well suited to the analysis of membrane proteins by circular dichroism (CD) and synchrotron radiation circular dichroism (SRCD). In this paper we have identified bicelle compositions that permit comparable studies of integral membrane proteins by solid-state NMR, CD, and SRCD; complementary methods that can provide insights into protein structure and the interactions with drugs and other small molecules. Furthermore we have been able to identify bicelle compositions that allow the magnetic alignment of the bicelles at fields routinely available in magnetic CD instruments. This potentially provides a route to the preparation of samples for oriented CD that mitigates many of the issues associated with the preparation of mechanically aligned samples, although we demonstrate that the dynamics present within the system can complicate the analysis of such spectra.
Bicelles, Cell membranes, Circular Dichroism, Magnetically alignable lipid phases, NMR, Synchrotron radiation circular dichroism
0005-2736
Evans, Luke S.
c888a996-eb12-4e6b-9c2b-e6cda907674a
Hussain, Rohanah
d46302e5-4734-4f59-9ac1-bc7b1f62693c
Siligardi, Giuliano
ebce490a-2faa-4b98-9703-ae0d6f8e46c2
Williamson, Philip T.F.
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a
Evans, Luke S.
c888a996-eb12-4e6b-9c2b-e6cda907674a
Hussain, Rohanah
d46302e5-4734-4f59-9ac1-bc7b1f62693c
Siligardi, Giuliano
ebce490a-2faa-4b98-9703-ae0d6f8e46c2
Williamson, Philip T.F.
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a

Evans, Luke S., Hussain, Rohanah, Siligardi, Giuliano and Williamson, Philip T.F. (2020) Magnetically aligned membrane mimetics enabling comparable chiroptical and magnetic resonance spectroscopy studies. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1862 (9), [183343]. (doi:10.1016/j.bbamem.2020.183343).

Record type: Article

Abstract

Lipidic bicelles have been widely used for the analysis of integral membrane proteins where their spontaneous alignment in an magnetic field has been exploited for oriented sample solid-state NMR studies. Many of their physical properties however make them well suited to the analysis of membrane proteins by circular dichroism (CD) and synchrotron radiation circular dichroism (SRCD). In this paper we have identified bicelle compositions that permit comparable studies of integral membrane proteins by solid-state NMR, CD, and SRCD; complementary methods that can provide insights into protein structure and the interactions with drugs and other small molecules. Furthermore we have been able to identify bicelle compositions that allow the magnetic alignment of the bicelles at fields routinely available in magnetic CD instruments. This potentially provides a route to the preparation of samples for oriented CD that mitigates many of the issues associated with the preparation of mechanically aligned samples, although we demonstrate that the dynamics present within the system can complicate the analysis of such spectra.

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Magnetically aligned membrane mimetics - Accepted Manuscript
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More information

Accepted/In Press date: 4 May 2020
e-pub ahead of print date: 14 May 2020
Published date: 27 May 2020
Keywords: Bicelles, Cell membranes, Circular Dichroism, Magnetically alignable lipid phases, NMR, Synchrotron radiation circular dichroism

Identifiers

Local EPrints ID: 441182
URI: http://eprints.soton.ac.uk/id/eprint/441182
ISSN: 0005-2736
PURE UUID: 77ed7228-7f4b-4e00-92fd-abeb1b2585e1
ORCID for Philip T.F. Williamson: ORCID iD orcid.org/0000-0002-0231-8640

Catalogue record

Date deposited: 04 Jun 2020 16:30
Last modified: 26 Nov 2021 06:34

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Contributors

Author: Luke S. Evans
Author: Rohanah Hussain
Author: Giuliano Siligardi

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