Combining multiplex immunohistochemistry with computer aided topographical and single-cell analysis to investigate the mechanism of spontaneous regression in a case of metastatic congenital neuroblastoma
Combining multiplex immunohistochemistry with computer aided topographical and single-cell analysis to investigate the mechanism of spontaneous regression in a case of metastatic congenital neuroblastoma
This is a description of a novel combination of chromogenic multiplex immunohistochemistry, digital pathology, computer-aided cell detection and topographical analysis of tumour tissue to allow detailed study of the immune infiltrate. This is applied to a rare clinical case, where a tumour sample is available from an infant with metastatic neuroblastoma at the point of spontaneous regression. This allowed detailed analysis of the immune infiltrate, including spatial distribution and phenotype of lymphoid and myeloid populations, with a distinction between heterogeneous areas within the intra- and extra-tumoural immune microenvironments. The mechanism of spontaneous regression in congenital neuroblastoma is poorly understood, but the data obtained suggested an immune mediated phenomenon, characterised by an adaptive T cell driven response with a significant delayed-type hypersensitivity (granulomatous) component.
neuroblastoma, spontaneous regression, tumour infiltrating lymphocytes
Hammouche, Dalia
ccfd09ef-9e71-4518-9dec-5972a80d4216
Easton, Alistair
69b30349-7d9d-4a32-9b58-9bda9d32fc26
Lopez, Monette
ddb501fb-a414-4b3f-87df-439b0b4c89d4
Gray, Juliet
12d5e17c-97bb-4d6d-8fc4-3914b730ed42
23 September 2020
Hammouche, Dalia
ccfd09ef-9e71-4518-9dec-5972a80d4216
Easton, Alistair
69b30349-7d9d-4a32-9b58-9bda9d32fc26
Lopez, Monette
ddb501fb-a414-4b3f-87df-439b0b4c89d4
Gray, Juliet
12d5e17c-97bb-4d6d-8fc4-3914b730ed42
Hammouche, Dalia, Easton, Alistair, Lopez, Monette and Gray, Juliet
(2020)
Combining multiplex immunohistochemistry with computer aided topographical and single-cell analysis to investigate the mechanism of spontaneous regression in a case of metastatic congenital neuroblastoma.
Case Reports and Images in Oncology.
(doi:10.47496/nl.CRIO.2020.01).
Abstract
This is a description of a novel combination of chromogenic multiplex immunohistochemistry, digital pathology, computer-aided cell detection and topographical analysis of tumour tissue to allow detailed study of the immune infiltrate. This is applied to a rare clinical case, where a tumour sample is available from an infant with metastatic neuroblastoma at the point of spontaneous regression. This allowed detailed analysis of the immune infiltrate, including spatial distribution and phenotype of lymphoid and myeloid populations, with a distinction between heterogeneous areas within the intra- and extra-tumoural immune microenvironments. The mechanism of spontaneous regression in congenital neuroblastoma is poorly understood, but the data obtained suggested an immune mediated phenomenon, characterised by an adaptive T cell driven response with a significant delayed-type hypersensitivity (granulomatous) component.
Text
NL-CRIO-2020-1-107
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Submitted date: 29 April 2020
Accepted/In Press date: 7 September 2020
Published date: 23 September 2020
Keywords:
neuroblastoma, spontaneous regression, tumour infiltrating lymphocytes
Identifiers
Local EPrints ID: 441192
URI: http://eprints.soton.ac.uk/id/eprint/441192
PURE UUID: e6d62b67-dc33-47f7-8b84-6cd199c2b9ea
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Date deposited: 04 Jun 2020 16:31
Last modified: 17 Mar 2024 05:36
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Contributors
Author:
Dalia Hammouche
Author:
Alistair Easton
Author:
Monette Lopez
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