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Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins

Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins
Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins
Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, ‘nativeomics’, unifying ‘omics’ (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.
Native mass spectrometry, top-down mass spectrometry, protein-ligand binding, membrane proteins, structural biology, endogenous ligands, Nativeomics, lipids, de-orphanisation
1548-7091
505-508
Gault, Joseph
c15d2b21-defd-4c42-bf46-2dfca93535d6
Liko, Idlir
10e8e8d2-fde8-42b7-8e05-ad18e9997323
Landreh, Michael
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Shutin, Denis
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Reddy Bolla, Jani
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Jefferies, Damien
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Agasid, Mark
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Yen, Hsin-Yung
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Ladds, Marcus J. G. W.
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Lane, David P.
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Khalid, Syma
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Mullen, Christopher
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Remes, Phil
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Huguet, Romain
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McAlister, Graeme
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Goodwin, Michael
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Viner, Rosa
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Skya, John
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Robinson, Carol V.
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Gault, Joseph
c15d2b21-defd-4c42-bf46-2dfca93535d6
Liko, Idlir
10e8e8d2-fde8-42b7-8e05-ad18e9997323
Landreh, Michael
08603478-f3b4-42ed-958d-ad9b5cdc1f4c
Shutin, Denis
2e2b2d52-8982-49cf-9383-bd712936e2a4
Reddy Bolla, Jani
2938cca2-6559-431c-a357-2bae109e6c75
Jefferies, Damien
8df97e21-8df6-4571-bfbe-3edc41e16967
Agasid, Mark
442c8273-1d46-4755-99eb-79f048a27d32
Yen, Hsin-Yung
c5a329c2-073d-4cfe-9ad5-b2a382f2795e
Ladds, Marcus J. G. W.
ee0e1b91-2551-453c-81d0-b2604d9c9c53
Lane, David P.
4a30dd01-4f98-4d97-887b-86186ab33518
Khalid, Syma
90fbd954-7248-4f47-9525-4d6af9636394
Mullen, Christopher
499f82bf-84f7-47f3-9a04-422ff4893f7e
Remes, Phil
d18704fa-2da0-407e-9994-e7c1c49b0241
Huguet, Romain
f492765e-e51f-4686-9da1-9ab6d8e5e67e
McAlister, Graeme
9ce62a07-b408-48ea-be8a-fef7fab625c0
Goodwin, Michael
243f5217-1ae3-4208-a137-f259590d3be5
Viner, Rosa
bb05f90d-2bd7-4905-bb09-d54df22b194c
Skya, John
f6f70002-42a3-4d96-ad77-26814fc3e4e0
Robinson, Carol V.
54050df3-1bf7-42d9-a5eb-c39e010f1bbe

Gault, Joseph, Liko, Idlir, Landreh, Michael, Shutin, Denis, Reddy Bolla, Jani, Jefferies, Damien, Agasid, Mark, Yen, Hsin-Yung, Ladds, Marcus J. G. W., Lane, David P., Khalid, Syma, Mullen, Christopher, Remes, Phil, Huguet, Romain, McAlister, Graeme, Goodwin, Michael, Viner, Rosa, Skya, John and Robinson, Carol V. (2020) Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins. Nature Methods, 505-508. (doi:10.1038/s41592-020-0821-0).

Record type: Article

Abstract

Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, ‘nativeomics’, unifying ‘omics’ (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.

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Nativeomics Paper Combined ACCEPTED - Accepted Manuscript
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More information

Accepted/In Press date: 25 March 2020
e-pub ahead of print date: 4 May 2020
Keywords: Native mass spectrometry, top-down mass spectrometry, protein-ligand binding, membrane proteins, structural biology, endogenous ligands, Nativeomics, lipids, de-orphanisation

Identifiers

Local EPrints ID: 441237
URI: http://eprints.soton.ac.uk/id/eprint/441237
ISSN: 1548-7091
PURE UUID: 74e054a2-b791-4b8b-9442-305f58b5e5da
ORCID for Syma Khalid: ORCID iD orcid.org/0000-0002-3694-5044

Catalogue record

Date deposited: 05 Jun 2020 16:31
Last modified: 04 Nov 2020 05:01

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Contributors

Author: Joseph Gault
Author: Idlir Liko
Author: Michael Landreh
Author: Denis Shutin
Author: Jani Reddy Bolla
Author: Damien Jefferies
Author: Mark Agasid
Author: Hsin-Yung Yen
Author: Marcus J. G. W. Ladds
Author: David P. Lane
Author: Syma Khalid ORCID iD
Author: Christopher Mullen
Author: Phil Remes
Author: Romain Huguet
Author: Graeme McAlister
Author: Michael Goodwin
Author: Rosa Viner
Author: John Skya
Author: Carol V. Robinson

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