A SAM-I riboswitch with the ability to sense and respond to uncharged initiator tRNA
A SAM-I riboswitch with the ability to sense and respond to uncharged initiator tRNA
All known riboswitches use their aptamer to senese one metabolite signal and their expression platform to regulate gene expression. Here, we characterize a SAM-I riboswitch (SAM-IXcc) from the Xanthomonas campestris that regulates methionine synthesis via the met operon. In vitro and in vivo experiments show that SAM-IXcc controls the met operon primarily at the translational level in response to cellular S-adenosylmethionine (SAM) levels. Biochemical and genetic data demonstrate that SAM-IXcc expression platform not only can repress gene expression in response to SAM binding to SAM-IXcc aptamer but also can sense and bind uncharged initiator Met tRNA, resulting in the sequestering of the anti-Shine-Dalgarno (SD) sequence and freeing the SD for translation initiation. These findings identify a SAM-I riboswitch with a dual functioning expression platform that regulates methionine synthesis through a previously unrecognized mechanism and discover a natural tRNA-sensing RNA element. This SAM-I riboswitch appears to be highly conserved in Xanthomonas species.
Tang, Dong-Jie
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Du, Xinyu
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Shi, Qiang
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Zhang, Jian-Ling
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He, Yuan-Ping
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Chen, Yan-Miao
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Ming, Zhenhua
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Wang, Dan
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Zhong, Wan-Ying
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Liang, Yu-Wei
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Liu, Jin-Yang
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Huang, Jian-Ming
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Zhong, Yun-Shi
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An, Shi-Qi
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Gu, Hongzhou
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Tang, Ji-Liang
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3 June 2020
Tang, Dong-Jie
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Du, Xinyu
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Shi, Qiang
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Zhang, Jian-Ling
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He, Yuan-Ping
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Chen, Yan-Miao
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Ming, Zhenhua
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Wang, Dan
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Zhong, Wan-Ying
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Liang, Yu-Wei
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Liu, Jin-Yang
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Huang, Jian-Ming
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Zhong, Yun-Shi
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An, Shi-Qi
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Gu, Hongzhou
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Tang, Ji-Liang
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Tang, Dong-Jie, Du, Xinyu, Shi, Qiang, Zhang, Jian-Ling, He, Yuan-Ping, Chen, Yan-Miao, Ming, Zhenhua, Wang, Dan, Zhong, Wan-Ying, Liang, Yu-Wei, Liu, Jin-Yang, Huang, Jian-Ming, Zhong, Yun-Shi, An, Shi-Qi, Gu, Hongzhou and Tang, Ji-Liang
(2020)
A SAM-I riboswitch with the ability to sense and respond to uncharged initiator tRNA.
Nature Communications, 11 (1), [2794].
(doi:10.1038/s41467-020-16417-z).
Abstract
All known riboswitches use their aptamer to senese one metabolite signal and their expression platform to regulate gene expression. Here, we characterize a SAM-I riboswitch (SAM-IXcc) from the Xanthomonas campestris that regulates methionine synthesis via the met operon. In vitro and in vivo experiments show that SAM-IXcc controls the met operon primarily at the translational level in response to cellular S-adenosylmethionine (SAM) levels. Biochemical and genetic data demonstrate that SAM-IXcc expression platform not only can repress gene expression in response to SAM binding to SAM-IXcc aptamer but also can sense and bind uncharged initiator Met tRNA, resulting in the sequestering of the anti-Shine-Dalgarno (SD) sequence and freeing the SD for translation initiation. These findings identify a SAM-I riboswitch with a dual functioning expression platform that regulates methionine synthesis through a previously unrecognized mechanism and discover a natural tRNA-sensing RNA element. This SAM-I riboswitch appears to be highly conserved in Xanthomonas species.
Text
A SAM-I riboswitch
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e-pub ahead of print date: 3 June 2020
Published date: 3 June 2020
Additional Information:
Funding Information:
We thank professor Zihe Rao (Tsinghua University, Beijing, China) for providing manual DNA sequencing advice for in-line probing analysis, professor Guoliang Xu (Shanghai Institute of Biochemistry and Cell Biology, CAS, Shanghai, China) for offering space and equipment for in-line probing experiments, and professor Sanshu Li (School of Biomedical Science, Huaqiao University, Quanzhou, China) for helpful discussions and constructive suggestions. This work was supported by the National Natural Science Foundation of China (31470237, 91859104, 81861138004, 21673050), the 973 Program of the Ministry of Science and Technology of China (2015CB150600), the Ba Gui Scholar Program of Guangxi Zhuang Autonomous Region of China (2014A002), and the Outstanding Clinical Discipline Project of Shanghai Pudong (PWYgy2018-08).
Publisher Copyright:
© 2020, The Author(s).
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Local EPrints ID: 441335
URI: http://eprints.soton.ac.uk/id/eprint/441335
ISSN: 2041-1723
PURE UUID: 7062f660-07bf-41a4-85a9-9b22fdc93ef2
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Date deposited: 10 Jun 2020 16:30
Last modified: 16 Mar 2024 08:08
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Contributors
Author:
Dong-Jie Tang
Author:
Xinyu Du
Author:
Qiang Shi
Author:
Jian-Ling Zhang
Author:
Yuan-Ping He
Author:
Yan-Miao Chen
Author:
Zhenhua Ming
Author:
Dan Wang
Author:
Wan-Ying Zhong
Author:
Yu-Wei Liang
Author:
Jin-Yang Liu
Author:
Jian-Ming Huang
Author:
Yun-Shi Zhong
Author:
Shi-Qi An
Author:
Hongzhou Gu
Author:
Ji-Liang Tang
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