Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formation
Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formation
Synthetic osteo‐promoting materials that are able to stimulate and accelerate bone formation without the addition of exogenous cells or growth factors represent a major opportunity for an aging world population. A co‐assembling system that integrates hyaluronic acid tyramine (HA‐Tyr ), bioactive peptide amphiphiles (GHK‐Cu2+), and Laponite (Lap ) to engineer hydrogels with physical, mechanical, and biomolecular signals that can be tuned to enhance bone regeneration is reported. The central design element of the multicomponent hydrogels is the integration of self‐assembly and enzyme‐mediated oxidative coupling to optimize structure and mechanical properties in combination with the incorporation of an osteo‐ and angio‐promoting segments to facilitate signaling. Spectroscopic techniques are used to confirm the interplay of orthogonal covalent and supramolecular interactions in multicomponent hydrogel formation. Furthermore, physico‐mechanical characterizations reveal that the multicomponent hydrogels exhibit improved compressive strength, stress relaxation profile, low swelling ratio, and retarded enzymatic degradation compared to the single component hydrogels. Applicability is validated in vitro using human mesenchymal stem cells and human umbilical vein endothelial cells, and in vivo using a rabbit maxillary sinus floor reconstruction model. Animals treated with the HA‐Tyr‐HA‐Tyr‐GHK‐Cu2+ hydrogels exhibit significantly enhanced bone formation relative to controls including the commercially available Bio‐Oss.
bone formation, cranio-maxillofacial surgery, multicomponent self-assembly, nanocomposite hydrogels, nanosilicates, self- assembling peptides
1-13
Okesola, Babatunde O.
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Ni, Shilei
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Derkus, Burak
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Galeano, Carles C.
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Hasan, Abshar
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Wu, Yuanhao
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Ramis, Jopeth
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Buttery, Lee
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Dawson, Jonathan I.
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D'Este, Matteo
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Oreffo, Richard O.C.
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Eglin, David
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Sun, Hongchen
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Mata, Alvaro
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3 April 2020
Okesola, Babatunde O.
45408cc8-db61-485a-807b-695e2301d8f5
Ni, Shilei
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Derkus, Burak
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Galeano, Carles C.
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Hasan, Abshar
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Wu, Yuanhao
020953e1-848a-4f74-8abc-bd532afa7600
Ramis, Jopeth
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Buttery, Lee
83e7e228-9f50-40e8-bf7d-e14b1a357cdf
Dawson, Jonathan I.
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D'Este, Matteo
66e669fa-9b51-4fac-b44b-3341cf6ac3bc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Eglin, David
41659588-43dd-4e79-b337-817da93d6386
Sun, Hongchen
26ca977a-48dd-47ce-8c0d-fe19b16f528a
Mata, Alvaro
c67ceb11-02c5-429c-a5e6-308c322f176b
Okesola, Babatunde O., Ni, Shilei, Derkus, Burak, Galeano, Carles C., Hasan, Abshar, Wu, Yuanhao, Ramis, Jopeth, Buttery, Lee, Dawson, Jonathan I., D'Este, Matteo, Oreffo, Richard O.C., Eglin, David, Sun, Hongchen and Mata, Alvaro
(2020)
Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formation.
Advanced Functional Materials, 30 (14), , [1906205].
(doi:10.1002/adfm.201906205).
Abstract
Synthetic osteo‐promoting materials that are able to stimulate and accelerate bone formation without the addition of exogenous cells or growth factors represent a major opportunity for an aging world population. A co‐assembling system that integrates hyaluronic acid tyramine (HA‐Tyr ), bioactive peptide amphiphiles (GHK‐Cu2+), and Laponite (Lap ) to engineer hydrogels with physical, mechanical, and biomolecular signals that can be tuned to enhance bone regeneration is reported. The central design element of the multicomponent hydrogels is the integration of self‐assembly and enzyme‐mediated oxidative coupling to optimize structure and mechanical properties in combination with the incorporation of an osteo‐ and angio‐promoting segments to facilitate signaling. Spectroscopic techniques are used to confirm the interplay of orthogonal covalent and supramolecular interactions in multicomponent hydrogel formation. Furthermore, physico‐mechanical characterizations reveal that the multicomponent hydrogels exhibit improved compressive strength, stress relaxation profile, low swelling ratio, and retarded enzymatic degradation compared to the single component hydrogels. Applicability is validated in vitro using human mesenchymal stem cells and human umbilical vein endothelial cells, and in vivo using a rabbit maxillary sinus floor reconstruction model. Animals treated with the HA‐Tyr‐HA‐Tyr‐GHK‐Cu2+ hydrogels exhibit significantly enhanced bone formation relative to controls including the commercially available Bio‐Oss.
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More information
e-pub ahead of print date: 16 February 2020
Published date: 3 April 2020
Additional Information:
Funding Information:
B.O.O. and S.N. contributed equally to this work. The work was supported by the ERC Starting Grant (STROFUNSCAFF) and the UK Regenerative Medicine Platform (UKRMP2) Acellular Smart Materials. R.O. and J.D. gratefully acknowledge funding support from the UK Regenerative Medicine Platform Hub Acellular SMART materials 3D architecture (MR/R015651/1) and the UK Regenerative Medicine Platform (MR/L012626/1 Southampton Imaging). D.E., M.E. and A.M. acknowledge funding support from AO Foundation (AOCMF‐17‐19M). J.D. thankfully acknowledge EPSRC for a fellowship (EP/L010259/1). H.S. acknowledge funding support from the National Key Research and Development Program of China (No. 2016YFC1102800) and the National Natural Science Foundation of China (No. 81870741). J.R. thanks Technological Institute of the Philippines for Ph.D. studentship. The authors thank Dr. Stephen Thorpe at the School of Engineering and Materials Science, QMUL for help with interpreting the mechanics of the hydrogels. They also thank Dr. Vicente Araullo‐Peters and Dr. Roberto Buccafusca at Nanovision and the School of Biological and Chemical Sciences (SBCS), QMUL for technical support.
Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords:
bone formation, cranio-maxillofacial surgery, multicomponent self-assembly, nanocomposite hydrogels, nanosilicates, self- assembling peptides
Identifiers
Local EPrints ID: 441425
URI: http://eprints.soton.ac.uk/id/eprint/441425
ISSN: 1616-301X
PURE UUID: 147c51e0-e1ea-4f1c-98e6-f5ca3b657da9
Catalogue record
Date deposited: 11 Jun 2020 16:41
Last modified: 17 Mar 2024 03:14
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Contributors
Author:
Babatunde O. Okesola
Author:
Shilei Ni
Author:
Burak Derkus
Author:
Carles C. Galeano
Author:
Abshar Hasan
Author:
Yuanhao Wu
Author:
Jopeth Ramis
Author:
Lee Buttery
Author:
Matteo D'Este
Author:
David Eglin
Author:
Hongchen Sun
Author:
Alvaro Mata
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