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Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formation

Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formation
Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formation
Synthetic osteo‐promoting materials that are able to stimulate and accelerate bone formation without the addition of exogenous cells or growth factors represent a major opportunity for an aging world population. A co‐assembling system that integrates hyaluronic acid tyramine (HA‐Tyr ), bioactive peptide amphiphiles (GHK‐Cu2+), and Laponite (Lap ) to engineer hydrogels with physical, mechanical, and biomolecular signals that can be tuned to enhance bone regeneration is reported. The central design element of the multicomponent hydrogels is the integration of self‐assembly and enzyme‐mediated oxidative coupling to optimize structure and mechanical properties in combination with the incorporation of an osteo‐ and angio‐promoting segments to facilitate signaling. Spectroscopic techniques are used to confirm the interplay of orthogonal covalent and supramolecular interactions in multicomponent hydrogel formation. Furthermore, physico‐mechanical characterizations reveal that the multicomponent hydrogels exhibit improved compressive strength, stress relaxation profile, low swelling ratio, and retarded enzymatic degradation compared to the single component hydrogels. Applicability is validated in vitro using human mesenchymal stem cells and human umbilical vein endothelial cells, and in vivo using a rabbit maxillary sinus floor reconstruction model. Animals treated with the HA‐Tyr‐HA‐Tyr‐GHK‐Cu2+ hydrogels exhibit significantly enhanced bone formation relative to controls including the commercially available Bio‐Oss.
bone formation, cranio-maxillofacial surgery, multicomponent self-assembly, nanocomposite hydrogels, nanosilicates, self- assembling peptides
1616-301X
1-13
Okesola, Babatunde O.
45408cc8-db61-485a-807b-695e2301d8f5
Ni, Shilei
9b4452c2-61f1-40e9-8ea4-7422d7ee99ec
Derkus, Burak
5a84f5e3-1531-4873-b6c8-c26da839b603
Galeano, Carles C.
601c533b-9da7-4576-88cd-02fd94901517
Hasan, Abshar
e848f7be-0ae4-4831-87da-81caddd92bcb
Wu, Yuanhao
020953e1-848a-4f74-8abc-bd532afa7600
Ramis, Jopeth
4ae03c71-5721-43a8-af61-81ea0148b4a3
Buttery, Lee
83e7e228-9f50-40e8-bf7d-e14b1a357cdf
Dawson, Jonathan I.
b220fe76-498d-47be-9995-92da6c289cf3
D'Este, Matteo
66e669fa-9b51-4fac-b44b-3341cf6ac3bc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Eglin, David
41659588-43dd-4e79-b337-817da93d6386
Sun, Hongchen
26ca977a-48dd-47ce-8c0d-fe19b16f528a
Mata, Alvaro
c67ceb11-02c5-429c-a5e6-308c322f176b
Okesola, Babatunde O.
45408cc8-db61-485a-807b-695e2301d8f5
Ni, Shilei
9b4452c2-61f1-40e9-8ea4-7422d7ee99ec
Derkus, Burak
5a84f5e3-1531-4873-b6c8-c26da839b603
Galeano, Carles C.
601c533b-9da7-4576-88cd-02fd94901517
Hasan, Abshar
e848f7be-0ae4-4831-87da-81caddd92bcb
Wu, Yuanhao
020953e1-848a-4f74-8abc-bd532afa7600
Ramis, Jopeth
4ae03c71-5721-43a8-af61-81ea0148b4a3
Buttery, Lee
83e7e228-9f50-40e8-bf7d-e14b1a357cdf
Dawson, Jonathan I.
b220fe76-498d-47be-9995-92da6c289cf3
D'Este, Matteo
66e669fa-9b51-4fac-b44b-3341cf6ac3bc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Eglin, David
41659588-43dd-4e79-b337-817da93d6386
Sun, Hongchen
26ca977a-48dd-47ce-8c0d-fe19b16f528a
Mata, Alvaro
c67ceb11-02c5-429c-a5e6-308c322f176b

Okesola, Babatunde O., Ni, Shilei, Derkus, Burak, Galeano, Carles C., Hasan, Abshar, Wu, Yuanhao, Ramis, Jopeth, Buttery, Lee, Dawson, Jonathan I., D'Este, Matteo, Oreffo, Richard O.C., Eglin, David, Sun, Hongchen and Mata, Alvaro (2020) Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formation. Advanced Functional Materials, 30 (14), 1-13, [1906205]. (doi:10.1002/adfm.201906205).

Record type: Article

Abstract

Synthetic osteo‐promoting materials that are able to stimulate and accelerate bone formation without the addition of exogenous cells or growth factors represent a major opportunity for an aging world population. A co‐assembling system that integrates hyaluronic acid tyramine (HA‐Tyr ), bioactive peptide amphiphiles (GHK‐Cu2+), and Laponite (Lap ) to engineer hydrogels with physical, mechanical, and biomolecular signals that can be tuned to enhance bone regeneration is reported. The central design element of the multicomponent hydrogels is the integration of self‐assembly and enzyme‐mediated oxidative coupling to optimize structure and mechanical properties in combination with the incorporation of an osteo‐ and angio‐promoting segments to facilitate signaling. Spectroscopic techniques are used to confirm the interplay of orthogonal covalent and supramolecular interactions in multicomponent hydrogel formation. Furthermore, physico‐mechanical characterizations reveal that the multicomponent hydrogels exhibit improved compressive strength, stress relaxation profile, low swelling ratio, and retarded enzymatic degradation compared to the single component hydrogels. Applicability is validated in vitro using human mesenchymal stem cells and human umbilical vein endothelial cells, and in vivo using a rabbit maxillary sinus floor reconstruction model. Animals treated with the HA‐Tyr‐HA‐Tyr‐GHK‐Cu2+ hydrogels exhibit significantly enhanced bone formation relative to controls including the commercially available Bio‐Oss.

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More information

e-pub ahead of print date: 16 February 2020
Published date: 3 April 2020
Keywords: bone formation, cranio-maxillofacial surgery, multicomponent self-assembly, nanocomposite hydrogels, nanosilicates, self- assembling peptides

Identifiers

Local EPrints ID: 441425
URI: http://eprints.soton.ac.uk/id/eprint/441425
ISSN: 1616-301X
PURE UUID: 147c51e0-e1ea-4f1c-98e6-f5ca3b657da9
ORCID for Jonathan I. Dawson: ORCID iD orcid.org/0000-0002-6712-0598
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 11 Jun 2020 16:41
Last modified: 26 Nov 2021 02:53

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Contributors

Author: Babatunde O. Okesola
Author: Shilei Ni
Author: Burak Derkus
Author: Carles C. Galeano
Author: Abshar Hasan
Author: Yuanhao Wu
Author: Jopeth Ramis
Author: Lee Buttery
Author: Matteo D'Este
Author: David Eglin
Author: Hongchen Sun
Author: Alvaro Mata

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