READ ME File For 'Data for Mechanism of Triterpenoid Saponin Mediated Augmentation of Immunotoxin Cytotoxicity'

Dataset DOI: 10.5258/SOTON/D1148

ReadMe Author: HARRISON WENSLEY, University of Southampton 

This dataset supports the thesis entitled 
Mechanism of Triterpenoid Saponin Mediated Augmentation of Immunotoxin Cytotoxicity
AWARDED BY: University of Southampton

This dataset includes experimental data used for generating figures in this thesis. Readers are referred to the thesis for descriptions of the methods used in generating this data and for definitions of terms used in these files.
Confocal microscopy data is contained in the .lif (Leica Image File) format. These can be opened with Leica brand software or with the ImageJ freeware image processing program.

This dataset contains:

Figure 4.xlsx	Fluorescence measurement for Daudi cells containing Fluo-8 recorded at 12s and 50s timepoints. Sheets include data for untreated controls and cells treated with SA at 1, 10, 20 and 40 µg/ml in D10 and CFD10 media.

Figure 5.xlsx	Absorbance data for XTT assays investigating the effect of calcium ion depletion and repletion with calcium chloride on the augmentation of OKT10-SAP cytotoxicity by SA

Figure 6.xlsx	Percentage of Daudi cells permeabilised by 0, 1, 10, 20, 30 and 40 µg/ml of SA in DMEM control and in the presence of EGTA.

Figure 7.lif		Confocal microscopy showing surface LAMP-1 expression in Daudi cells

Figure 7.xlsx	Mean fluorescence intensity determined by flow cytometry for Daudi cells probed with anti-LAMP-1 Ab

Figure 8.lif		Confocal microscopy showing surface ceramide expression in Daudi cells

Figure 8.xlsx	Mean fluorescence intensity determined by flow cytometry for Daudi cells probed with anti-ceramide Ab

Figure 9.xlsx	Absorbance data for XTT assays investigating the toxicity of amitriptyline in Daudi cells

Figure 10.xlsx	Absorbance data for XTT assays investigating the effect of amitriptyline on the augmentation of OKT10-SAP cytotoxicity by SA

Figure 15.xlsx	Absorbance data for XTT assays investigating the toxicity of a number of pharmacological agents in Daudi cells

Figure 16.xlsx	Absorbance data for XTT assays optimising the concentration of pharmacological agents in relation to their effect on the cytotoxicity of OKT10-SAP in the absence and presence of SA

Figure 17.xlsx	Absorbance data for XTT assays investigating the effect of pharmacological agents on the augmentation of OKT10-SAP cytotoxicity by SA in Daudi cells

Figure 18.xlsx	Absorbance data for XTT assays investigating the effect of pharmacological agents on the augmentation of Saporin cytotoxicity by SA in Daudi cells

Figure 19.xlsx	Absorbance data for XTT assays investigating the effect of pharmacological agents on the augmentation of OKT10-SAP cytotoxicity by SA in HSB-2 cells

Figure 20.xlsx	Absorbance data for XTT assays investigating the effect of pharmacological agents on the augmentation of Saporin cytotoxicity by SA in HSB-2 cells

Figure 21.xlsx	Absorbance data for XTT assays investigating the augmentation of Gelonin cytotoxicity by SA

Figure 22.xlsx	Absorbance data for XTT assays investigating the cytotoxicity of the fluorescent conjugates SAP-AF and OKSAP-AF

Figure 23.xlsx	Absorbance data for OKSAP-AF binding ELISA

Figures 33 + 34.xlsx	Median FITC-W and FITC-H values from flow cytometry experiments investigating the uptake of OKSAP-AF and SAP-AF in Daudi and HSB-2 cells

Figure 37.xlsx	Median FITC-W values from flow cytometry experiments investigating the endolysosomal escape of SAP-AF in Daudi cells

Figure 39.xlsx	Median FITC-W values from flow cytometry experiments investigating the endolysosomal escape of OKSAP-AF in Daudi cells

Figure 40.xlsx	Median FITC-W values from flow cytometry experiments investigating the endolysosomal escape of SAP-AF in HSB-2 cells

Figure 42.xlsx	Median FITC-W values from flow cytometry experiments investigating the endolysosomal escape of OKSAP-AF in HSB-2 cells

For Confocal 1.lif, Confocal 2.lif, Confocal 3.lif and Colocalisation Confocal.lif, see Confocal Imaging Index.rtf for index of images

Date of data collection: 1/11/16 - 1/11/19

Geographic location of data collection: Simon Flavell Research Unit, Southampton General Hospital

License: CC BY

Funding was provided by: Leukaemia Busters
Registered Charity 1157147

November, 2019