In vitro effects of live and heat-inactivated Bifidobacterium animalis subsp. lactis, BB-12 and Lactobacillus rhamnosus GG on Caco-2 cells
In vitro effects of live and heat-inactivated Bifidobacterium animalis subsp. lactis, BB-12 and Lactobacillus rhamnosus GG on Caco-2 cells
Probiotic–host interaction can be cell-to-cell or through metabolite production. Dead (inactive) organisms could interact with the host, leading to local effects and possible health benefits. This research examined the effects of live and heat-inactivated Bifidobacterium animalis subsp. lactis, BB-12 (BB-12) and Lactobacillus rhamnosus GG (LGG) on cultured Caco-2 cells focusing on epithelial integrity and production of inflammatory mediators. Live organisms increased transepithelial electrical resistance (TEER), a barrier-integrity marker, with LGG having a greater effect than BB-12. When mildly heat-treated, both organisms had a more modest effect on TEER than when alive. When they were heat-inactivated, both organisms had only a limited effect on TEER. Neither live nor heat-inactivated organisms affected production of six inflammatory mediators produced by Caco-2 cells compared to control conditions. Pre-treatment with heat-inactivated LGG or BB-12 did not alter the decline in TEER caused by exposure to an inflammatory cocktail of cytokines. However, pre-treatment of Caco-2 cells with heat-inactivated organisms alone or their combination decreased the production of interleukin (IL)-6, IL-18, and vascular endothelial growth factor. To conclude, while the live organisms improve the epithelial barrier using this model, neither live nor heat-inactivated organisms directly elicit an inflammatory response by the epithelium. Pre-treatment with heat-inactivated BB-12 or LGG can reduce some components of the response induced by an inflammatory stimulus.
B. Animalis subsp, BB-12, Gut epithelium, Heat-inactivation, Inflammation, L. Rhamnosus GG, Lactis, Probiotic
Castro Herrera, Vivian Maritza
ee06e5c1-815f-497f-9c67-47ead3772704
Rasmussen, Christine
5c7270f0-453c-4622-98a7-51d006b52d53
Wellejus, Anja
b03ecf04-d89c-496b-a29c-48f7df4c86b3
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
8 June 2020
Castro Herrera, Vivian Maritza
ee06e5c1-815f-497f-9c67-47ead3772704
Rasmussen, Christine
5c7270f0-453c-4622-98a7-51d006b52d53
Wellejus, Anja
b03ecf04-d89c-496b-a29c-48f7df4c86b3
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Castro Herrera, Vivian Maritza, Rasmussen, Christine, Wellejus, Anja, Miles, Elizabeth and Calder, Philip
(2020)
In vitro effects of live and heat-inactivated Bifidobacterium animalis subsp. lactis, BB-12 and Lactobacillus rhamnosus GG on Caco-2 cells.
Nutrients, 12 (6), [1719].
(doi:10.3390/nu12061719).
Abstract
Probiotic–host interaction can be cell-to-cell or through metabolite production. Dead (inactive) organisms could interact with the host, leading to local effects and possible health benefits. This research examined the effects of live and heat-inactivated Bifidobacterium animalis subsp. lactis, BB-12 (BB-12) and Lactobacillus rhamnosus GG (LGG) on cultured Caco-2 cells focusing on epithelial integrity and production of inflammatory mediators. Live organisms increased transepithelial electrical resistance (TEER), a barrier-integrity marker, with LGG having a greater effect than BB-12. When mildly heat-treated, both organisms had a more modest effect on TEER than when alive. When they were heat-inactivated, both organisms had only a limited effect on TEER. Neither live nor heat-inactivated organisms affected production of six inflammatory mediators produced by Caco-2 cells compared to control conditions. Pre-treatment with heat-inactivated LGG or BB-12 did not alter the decline in TEER caused by exposure to an inflammatory cocktail of cytokines. However, pre-treatment of Caco-2 cells with heat-inactivated organisms alone or their combination decreased the production of interleukin (IL)-6, IL-18, and vascular endothelial growth factor. To conclude, while the live organisms improve the epithelial barrier using this model, neither live nor heat-inactivated organisms directly elicit an inflammatory response by the epithelium. Pre-treatment with heat-inactivated BB-12 or LGG can reduce some components of the response induced by an inflammatory stimulus.
Text
Castro-Herrera et al_Accepted Version_Nutrients
- Accepted Manuscript
More information
Accepted/In Press date: 5 June 2020
e-pub ahead of print date: 8 June 2020
Published date: 8 June 2020
Additional Information:
Funding Information:
Funding: V.M.C.-H. was funded by Colciencias, Colombia.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords:
B. Animalis subsp, BB-12, Gut epithelium, Heat-inactivation, Inflammation, L. Rhamnosus GG, Lactis, Probiotic
Identifiers
Local EPrints ID: 441450
URI: http://eprints.soton.ac.uk/id/eprint/441450
ISSN: 2072-6643
PURE UUID: ba438bed-ee1d-42dd-ae69-cef7cda2bef7
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Date deposited: 12 Jun 2020 16:40
Last modified: 17 Mar 2024 02:42
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Author:
Vivian Maritza Castro Herrera
Author:
Christine Rasmussen
Author:
Anja Wellejus
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