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Single platelet variability governs population sensitivity and initiates intrinsic heterotypic responses

Single platelet variability governs population sensitivity and initiates intrinsic heterotypic responses
Single platelet variability governs population sensitivity and initiates intrinsic heterotypic responses
Investigations into the nature of platelet functional variety and consequences for homeostasis require new methods for resolving single platelet phenotypes. Here we combine droplet microfluidics with flow cytometry for high throughput single platelet function analysis. A large-scale sensitivity continuum was shown to be a general feature of human platelets from individual donors, with hypersensitive platelets coordinating significant sensitivity gains in bulk platelet populations and shown to direct aggregation in droplet-confined minimal platelet systems. Sensitivity gains scaled with agonist potency (convulxin > TRAP-14>ADP) and reduced the collagen and thrombin activation threshold required for platelet population polarization into pro-aggregatory and pro-coagulant states. The heterotypic platelet response results from an intrinsic behavioural program. The method and findings invite future discoveries into the nature of hypersensitive platelets and how community effects produce population level responses in health and disease.
2399-3642
Jongen, Maaike Sybilla Anna
47c19196-eb09-421c-ab3a-1330ff9a79b0
Macarthur, Benjamin
2c0476e7-5d3e-4064-81bb-104e8e88bb6b
Englyst, Nicola
f84399af-7265-4224-b556-102c3aa272b0
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968
Jongen, Maaike Sybilla Anna
47c19196-eb09-421c-ab3a-1330ff9a79b0
Macarthur, Benjamin
2c0476e7-5d3e-4064-81bb-104e8e88bb6b
Englyst, Nicola
f84399af-7265-4224-b556-102c3aa272b0
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968

Jongen, Maaike Sybilla Anna, Macarthur, Benjamin, Englyst, Nicola and West, Jonathan (2020) Single platelet variability governs population sensitivity and initiates intrinsic heterotypic responses. Communications Biology, 3 (281), [281]. (doi:10.1038/s42003-020-1002-5).

Record type: Article

Abstract

Investigations into the nature of platelet functional variety and consequences for homeostasis require new methods for resolving single platelet phenotypes. Here we combine droplet microfluidics with flow cytometry for high throughput single platelet function analysis. A large-scale sensitivity continuum was shown to be a general feature of human platelets from individual donors, with hypersensitive platelets coordinating significant sensitivity gains in bulk platelet populations and shown to direct aggregation in droplet-confined minimal platelet systems. Sensitivity gains scaled with agonist potency (convulxin > TRAP-14>ADP) and reduced the collagen and thrombin activation threshold required for platelet population polarization into pro-aggregatory and pro-coagulant states. The heterotypic platelet response results from an intrinsic behavioural program. The method and findings invite future discoveries into the nature of hypersensitive platelets and how community effects produce population level responses in health and disease.

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Accepted/In Press date: 12 May 2020
e-pub ahead of print date: 4 June 2020
Published date: 4 June 2020

Identifiers

Local EPrints ID: 441503
URI: http://eprints.soton.ac.uk/id/eprint/441503
ISSN: 2399-3642
PURE UUID: e28368ce-e67f-47f8-bdac-1c8c63e9b8ab
ORCID for Nicola Englyst: ORCID iD orcid.org/0000-0003-0508-8323
ORCID for Jonathan West: ORCID iD orcid.org/0000-0002-5709-6790

Catalogue record

Date deposited: 16 Jun 2020 16:31
Last modified: 26 Nov 2021 02:59

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Contributors

Author: Maaike Sybilla Anna Jongen
Author: Nicola Englyst ORCID iD
Author: Jonathan West ORCID iD

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