Development of ultrahigh‐performance liquid chromatography/mass spectrometry and ultrahigh‐performance supercritical fluid chromatography/mass spectrometry assays to determine the concentration of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions
Development of ultrahigh‐performance liquid chromatography/mass spectrometry and ultrahigh‐performance supercritical fluid chromatography/mass spectrometry assays to determine the concentration of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions
Rationale: Fast and easily transferable chromatography/mass spectrometry assays were required to detect and quantify the amount of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions. Methods: Bitrex™ solutions were analysed using reversed-phase ultrahigh-performance liquid chromatography coupled with positive ion electrospray ionisation mass spectrometry (UHPLC/ESI-MS). Separation was achieved using a mobile phase gradient with an Acquity BEH C18-packed column. Sodium saccharin solutions were analysed using ultrahigh-performance supercritical fluid chromatography coupled with negative ion electrospray ionisation (UHPSFC/ESI-MS). Separation was achieved using isocratic elution with an Acquity UPC 2 Torus Diol packed column and a methanol (25 mM ammonium acetate) co-solvent. Results: The calibration curves obtained using the ratio of the active compound to an internal standard generated linear regression values (R 2) >0.99. Samples analysed prior to and after an autoclave sterilisation process and bottling gave repeatable measurements within 10% of the expected concentration. Conclusions: The two assays afford a fast robust and quantitative analytical method for the detection of the active components used to test the efficacy of the homemade facemask testing solutions.
Herniman, Julie M.
530b1a36-1386-4602-8df7-defa6eb3512b
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea
30 August 2020
Herniman, Julie M.
530b1a36-1386-4602-8df7-defa6eb3512b
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea
Herniman, Julie M. and Langley, G. John
(2020)
Development of ultrahigh‐performance liquid chromatography/mass spectrometry and ultrahigh‐performance supercritical fluid chromatography/mass spectrometry assays to determine the concentration of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions.
Rapid Communications in Mass Spectrometry, 34 (16), [e8848].
(doi:10.1002/rcm.8848).
Abstract
Rationale: Fast and easily transferable chromatography/mass spectrometry assays were required to detect and quantify the amount of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions. Methods: Bitrex™ solutions were analysed using reversed-phase ultrahigh-performance liquid chromatography coupled with positive ion electrospray ionisation mass spectrometry (UHPLC/ESI-MS). Separation was achieved using a mobile phase gradient with an Acquity BEH C18-packed column. Sodium saccharin solutions were analysed using ultrahigh-performance supercritical fluid chromatography coupled with negative ion electrospray ionisation (UHPSFC/ESI-MS). Separation was achieved using isocratic elution with an Acquity UPC 2 Torus Diol packed column and a methanol (25 mM ammonium acetate) co-solvent. Results: The calibration curves obtained using the ratio of the active compound to an internal standard generated linear regression values (R 2) >0.99. Samples analysed prior to and after an autoclave sterilisation process and bottling gave repeatable measurements within 10% of the expected concentration. Conclusions: The two assays afford a fast robust and quantitative analytical method for the detection of the active components used to test the efficacy of the homemade facemask testing solutions.
Text
UHPSFC and UHPLC-MS Bitrex and saccharine
- Accepted Manuscript
Available under License Other.
More information
Accepted/In Press date: 1 June 2020
e-pub ahead of print date: 3 June 2020
Published date: 30 August 2020
Additional Information:
Funding Information:
The authors thank Sensient Flavors Europe (Dr Lewis Jones), Nichols plc (Richard Nicolson) Vimto Soft Drinks and Nutraceuticals Group Europe for provision of sodium saccharin. Dr Sam Ferries at Waters Corporation provided training and the initial TargetLynx method. The preparation of the solutions was designed and completed by staff at the School of Chemistry, University of Southampton, Professor Steve Goldup, Dr Matthias Baud and members of their research groups. The sterilisation and bottling methods were developed and undertaken by Professor Sumeet Mahajan and Peter Johnson. Molly Wilson and Dr Tom Sutton provided assistance with QC calibration and sample preparation. This MisSO project was co-ordinated by Professor Delphine Boche, School of Medicine, University of Southampton (https://www.southampton.ac.uk/news/2020/04/misson-ppe-solution.page).
Publisher Copyright:
© 2020 The Authors. Rapid Communications in Mass Spectrometry published by John Wiley & Sons Ltd
Identifiers
Local EPrints ID: 441553
URI: http://eprints.soton.ac.uk/id/eprint/441553
ISSN: 0951-4198
PURE UUID: fc003621-9fc1-4db9-887f-9ed984de26a5
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Date deposited: 17 Jun 2020 16:31
Last modified: 28 Aug 2024 01:36
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