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Development of ultrahigh‐performance liquid chromatography‐ mass spectrometry and ultrahigh‐performance supercritical fluid chromatography‐mass spectrometry assays to determine the concentration of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions

Development of ultrahigh‐performance liquid chromatography‐ mass spectrometry and ultrahigh‐performance supercritical fluid chromatography‐mass spectrometry assays to determine the concentration of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions
Development of ultrahigh‐performance liquid chromatography‐ mass spectrometry and ultrahigh‐performance supercritical fluid chromatography‐mass spectrometry assays to determine the concentration of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions
Rationale
Fast and easily transferable chromatography‐mass spectrometry assays were required to detect and quantify the amount of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions.

Methods
Bitrex™ solutions were analysed using reversed phase ultrahigh‐performance liquid chromatography coupled with positive ion electrospray ionisation mass spectrometry (UHPLC/MS). Separation was achieved using a mobile phase gradient with an Acquity BEH C18 packed column. Sodium saccharin solutions were analysed using ultrahigh‐performance supercritical fluid chromatography coupled with negative ion electrospray ionisation (UHPSFC/MS). Separation was achieved using isocratic elution with an Acquity UPC2 Torus Diol packed column and a methanol (25 mM ammonium acetate) co‐solvent.

Results
The calibration curves obtained using the ratio of the active compound an internal standard generated linear regression values (R2) > 0.99. Samples analysed prior to and after an autoclave sterilisation process and bottling give repeatable measurements within 10% of the expected concentration.

Conclusions
The two assays afford a fast robust and quantitative analytical method for the detection of the active components used to test the efficacy of the homemade facemask testing solutions.
0951-4198
Herniman, Julie
530b1a36-1386-4602-8df7-defa6eb3512b
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea
Herniman, Julie
530b1a36-1386-4602-8df7-defa6eb3512b
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea

Herniman, Julie and Langley, G. John (2020) Development of ultrahigh‐performance liquid chromatography‐ mass spectrometry and ultrahigh‐performance supercritical fluid chromatography‐mass spectrometry assays to determine the concentration of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions. Rapid Communications in Mass Spectrometry, 34 (16), [e8848]. (doi:10.1002/rcm.8848).

Record type: Article

Abstract

Rationale
Fast and easily transferable chromatography‐mass spectrometry assays were required to detect and quantify the amount of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions.

Methods
Bitrex™ solutions were analysed using reversed phase ultrahigh‐performance liquid chromatography coupled with positive ion electrospray ionisation mass spectrometry (UHPLC/MS). Separation was achieved using a mobile phase gradient with an Acquity BEH C18 packed column. Sodium saccharin solutions were analysed using ultrahigh‐performance supercritical fluid chromatography coupled with negative ion electrospray ionisation (UHPSFC/MS). Separation was achieved using isocratic elution with an Acquity UPC2 Torus Diol packed column and a methanol (25 mM ammonium acetate) co‐solvent.

Results
The calibration curves obtained using the ratio of the active compound an internal standard generated linear regression values (R2) > 0.99. Samples analysed prior to and after an autoclave sterilisation process and bottling give repeatable measurements within 10% of the expected concentration.

Conclusions
The two assays afford a fast robust and quantitative analytical method for the detection of the active components used to test the efficacy of the homemade facemask testing solutions.

Text
UHPSFC and UHPLC-MS Bitrex and saccharine - Accepted Manuscript
Available under License Other.
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More information

Accepted/In Press date: 1 June 2020
e-pub ahead of print date: 3 June 2020

Identifiers

Local EPrints ID: 441553
URI: http://eprints.soton.ac.uk/id/eprint/441553
ISSN: 0951-4198
PURE UUID: fc003621-9fc1-4db9-887f-9ed984de26a5
ORCID for Julie Herniman: ORCID iD orcid.org/0000-0003-4834-1093
ORCID for G. John Langley: ORCID iD orcid.org/0000-0002-8323-7235

Catalogue record

Date deposited: 17 Jun 2020 16:31
Last modified: 26 Nov 2021 02:41

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