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Spatiotemporal analysis of a glycolytic activity gradient linked to mouse embryo mesoderm development

Spatiotemporal analysis of a glycolytic activity gradient linked to mouse embryo mesoderm development
Spatiotemporal analysis of a glycolytic activity gradient linked to mouse embryo mesoderm development
How metabolism is rewired during embryonic development is still largely unknown, as it remains a major technical challenge to resolve metabolic activities or metabolite levels with spatiotemporal resolution. Here, we investigated metabolic changes during development of organogenesis-stage mouse embryos, focusing on the presomitic mesoderm (PSM). We measured glycolytic labeling kinetics from 13C-glucose tracing experiments and detected elevated glycolysis in the posterior, more undifferentiated PSM. We found evidence that the spatial metabolic differences are functionally relevant during PSM development. To enable real-time quantification of a glycolytic metabolite with spatiotemporal resolution, we generated a pyruvate FRET-sensor reporter mouse line. We revealed dynamic changes in cytosolic pyruvate levels as cells transit toward a more anterior PSM state. Combined, our approach identifies a gradient of glycolytic activity across the PSM, and we provide evidence that these spatiotemporal metabolic changes are intrinsically linked to PSM development and differentiation.
1534-5807
331-341.e4
Bulusu, Vinay
46b0e765-f388-4445-a855-4f85218a655b
Prior, Nicole
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Snaebjornsson, Marteinn T.
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Kuehne, Andreas
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Sonnen, Katharina F.
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Kress, Jana
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Stein, Frank
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Schultz, Carsten
cc8a7ed9-9937-43b6-ad96-8ce99b704064
Sauer, Uwe
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Aulehla, Alexander
6fefa280-b37c-485d-b714-2581e8233d14
Bulusu, Vinay
46b0e765-f388-4445-a855-4f85218a655b
Prior, Nicole
27851f03-6041-4409-a15c-a2a164b883e0
Snaebjornsson, Marteinn T.
9ccefe91-9eb2-4b76-a28d-09350ac60b37
Kuehne, Andreas
df0d73b2-d621-4167-b9db-2eb396fa2d4c
Sonnen, Katharina F.
94fc49f0-6a63-4a0f-8d44-72484ccef268
Kress, Jana
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Stein, Frank
bfe49b25-6556-4bae-a2e8-06f98fdf4ac1
Schultz, Carsten
cc8a7ed9-9937-43b6-ad96-8ce99b704064
Sauer, Uwe
c5ed15ab-afda-4efb-80f7-319b268324b1
Aulehla, Alexander
6fefa280-b37c-485d-b714-2581e8233d14

Bulusu, Vinay, Prior, Nicole, Snaebjornsson, Marteinn T., Kuehne, Andreas, Sonnen, Katharina F., Kress, Jana, Stein, Frank, Schultz, Carsten, Sauer, Uwe and Aulehla, Alexander (2017) Spatiotemporal analysis of a glycolytic activity gradient linked to mouse embryo mesoderm development. Developmental Cell, 40 (4), 331-341.e4. (doi:10.1016/j.devcel.2017.01.015).

Record type: Article

Abstract

How metabolism is rewired during embryonic development is still largely unknown, as it remains a major technical challenge to resolve metabolic activities or metabolite levels with spatiotemporal resolution. Here, we investigated metabolic changes during development of organogenesis-stage mouse embryos, focusing on the presomitic mesoderm (PSM). We measured glycolytic labeling kinetics from 13C-glucose tracing experiments and detected elevated glycolysis in the posterior, more undifferentiated PSM. We found evidence that the spatial metabolic differences are functionally relevant during PSM development. To enable real-time quantification of a glycolytic metabolite with spatiotemporal resolution, we generated a pyruvate FRET-sensor reporter mouse line. We revealed dynamic changes in cytosolic pyruvate levels as cells transit toward a more anterior PSM state. Combined, our approach identifies a gradient of glycolytic activity across the PSM, and we provide evidence that these spatiotemporal metabolic changes are intrinsically linked to PSM development and differentiation.

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Spatiotemporal Analysis of a Glycolytic Activity - Version of Record
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More information

Accepted/In Press date: 27 January 2017
e-pub ahead of print date: 27 February 2017
Published date: 27 February 2017

Identifiers

Local EPrints ID: 441625
URI: http://eprints.soton.ac.uk/id/eprint/441625
ISSN: 1534-5807
PURE UUID: 8eccceda-112b-4ddc-88be-9a00fc4b0387
ORCID for Nicole Prior: ORCID iD orcid.org/0000-0003-2856-7052

Catalogue record

Date deposited: 22 Jun 2020 16:30
Last modified: 16 Nov 2021 03:06

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Contributors

Author: Vinay Bulusu
Author: Nicole Prior ORCID iD
Author: Marteinn T. Snaebjornsson
Author: Andreas Kuehne
Author: Katharina F. Sonnen
Author: Jana Kress
Author: Frank Stein
Author: Carsten Schultz
Author: Uwe Sauer
Author: Alexander Aulehla

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