DNA: Gold nanoparticles designed for mRNA sensing in cells: imaging of the gold nanoparticles using two photon photoluminescence spectroscopy
DNA: Gold nanoparticles designed for mRNA sensing in cells: imaging of the gold nanoparticles using two photon photoluminescence spectroscopy
Recently DNA-coated gold nanoparticles have emerged as ideal tools for the detection of mRNA in cells using dye modified oligonucleotides. However, the tracking of the gold core has been hindered by the small size of the particle core. In this work we utilize a home built set up and 43 nm DNA-coated spherical gold nanoparticles for the simultaneous imaging of mRNA detection using fluorescence microscopy and the gold nanoparticle core using two photon photoluminescence (TPPL).
Cancer, Colloids, DNA, Drugs, mRNA, Nanoparticles, TPPL
Kyriazi, Maria Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
7 March 2019
Kyriazi, Maria Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Kyriazi, Maria Eleni, Muskens, Otto L. and Kanaras, Antonios G.
(2019)
DNA: Gold nanoparticles designed for mRNA sensing in cells: imaging of the gold nanoparticles using two photon photoluminescence spectroscopy.
Osinski, Marek and Parak, Wolfgang J.
(eds.)
In Colloidal Nanoparticles for Biomedical Applications XIV.
vol. 10892,
SPIE..
(doi:10.1117/12.2502724).
Record type:
Conference or Workshop Item
(Paper)
Abstract
Recently DNA-coated gold nanoparticles have emerged as ideal tools for the detection of mRNA in cells using dye modified oligonucleotides. However, the tracking of the gold core has been hindered by the small size of the particle core. In this work we utilize a home built set up and 43 nm DNA-coated spherical gold nanoparticles for the simultaneous imaging of mRNA detection using fluorescence microscopy and the gold nanoparticle core using two photon photoluminescence (TPPL).
Text
Kyriazi_kanaras_SPIE_2019
- Accepted Manuscript
More information
Published date: 7 March 2019
Venue - Dates:
Colloidal Nanoparticles for Biomedical Applications XIV 2019, , San Francisco, United States, 2019-02-02 - 2019-02-04
Keywords:
Cancer, Colloids, DNA, Drugs, mRNA, Nanoparticles, TPPL
Identifiers
Local EPrints ID: 441651
URI: http://eprints.soton.ac.uk/id/eprint/441651
ISSN: 1996-756X
PURE UUID: 137ffcc3-5083-4d71-8c65-bfaeca2fefd2
Catalogue record
Date deposited: 23 Jun 2020 16:30
Last modified: 17 Mar 2024 03:18
Export record
Altmetrics
Contributors
Author:
Maria Eleni Kyriazi
Editor:
Marek Osinski
Editor:
Wolfgang J. Parak
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
