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DNA: Gold nanoparticles designed for mRNA sensing in cells: imaging of the gold nanoparticles using two photon photoluminescence spectroscopy

DNA: Gold nanoparticles designed for mRNA sensing in cells: imaging of the gold nanoparticles using two photon photoluminescence spectroscopy
DNA: Gold nanoparticles designed for mRNA sensing in cells: imaging of the gold nanoparticles using two photon photoluminescence spectroscopy

Recently DNA-coated gold nanoparticles have emerged as ideal tools for the detection of mRNA in cells using dye modified oligonucleotides. However, the tracking of the gold core has been hindered by the small size of the particle core. In this work we utilize a home built set up and 43 nm DNA-coated spherical gold nanoparticles for the simultaneous imaging of mRNA detection using fluorescence microscopy and the gold nanoparticle core using two photon photoluminescence (TPPL).

Cancer, Colloids, DNA, Drugs, mRNA, Nanoparticles, TPPL
1996-756X
SPIE
Kyriazi, Maria Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Osinski, Marek
Parak, Wolfgang J.
Kyriazi, Maria Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Osinski, Marek
Parak, Wolfgang J.

Kyriazi, Maria Eleni, Muskens, Otto L. and Kanaras, Antonios G. (2019) DNA: Gold nanoparticles designed for mRNA sensing in cells: imaging of the gold nanoparticles using two photon photoluminescence spectroscopy. Osinski, Marek and Parak, Wolfgang J. (eds.) In Colloidal Nanoparticles for Biomedical Applications XIV. vol. 10892, SPIE.. (doi:10.1117/12.2502724).

Record type: Conference or Workshop Item (Paper)

Abstract

Recently DNA-coated gold nanoparticles have emerged as ideal tools for the detection of mRNA in cells using dye modified oligonucleotides. However, the tracking of the gold core has been hindered by the small size of the particle core. In this work we utilize a home built set up and 43 nm DNA-coated spherical gold nanoparticles for the simultaneous imaging of mRNA detection using fluorescence microscopy and the gold nanoparticle core using two photon photoluminescence (TPPL).

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Kyriazi_kanaras_SPIE_2019 - Accepted Manuscript
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More information

Published date: 7 March 2019
Venue - Dates: Colloidal Nanoparticles for Biomedical Applications XIV 2019, , San Francisco, United States, 2019-02-02 - 2019-02-04
Keywords: Cancer, Colloids, DNA, Drugs, mRNA, Nanoparticles, TPPL

Identifiers

Local EPrints ID: 441651
URI: http://eprints.soton.ac.uk/id/eprint/441651
ISSN: 1996-756X
PURE UUID: 137ffcc3-5083-4d71-8c65-bfaeca2fefd2
ORCID for Otto L. Muskens: ORCID iD orcid.org/0000-0003-0693-5504
ORCID for Antonios G. Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

Catalogue record

Date deposited: 23 Jun 2020 16:30
Last modified: 26 Nov 2021 02:54

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Contributors

Author: Maria Eleni Kyriazi
Author: Otto L. Muskens ORCID iD
Editor: Marek Osinski
Editor: Wolfgang J. Parak

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