The University of Southampton
University of Southampton Institutional Repository

Far-field unlabelled super-resolution imaging with superoscillatory illumination

Far-field unlabelled super-resolution imaging with superoscillatory illumination
Far-field unlabelled super-resolution imaging with superoscillatory illumination
Unlabeled super-resolution is the next grand challenge in imaging. Stimulated emission depletion and single-molecule microscopies have revolutionized the life sciences but are still limited by the need for reporters (labels) embedded within the sample. While the Veselago–Pendry “super-lens,” using a negative-index metamaterial, is a promising idea for imaging beyond the diffraction limit, there are substantial technological challenges to its realization. Another route to far-field subwavelength focusing is using optical superoscillations: engineered interference of multiple coherent waves creating an, in principle, arbitrarily small hotspot. Here, we demonstrate microscopy with superoscillatory illumination of the object and describe its underlying principles. We show that far-field images taken with superoscillatory illumination are themselves superoscillatory and, hence, can reveal fine structural details of the object that are lost in conventional far-field imaging. We show that the resolution of a superoscillatory microscope is determined by the size of the hotspot, rather than the bandwidth of the optical instrument. We demonstrate high-frame-rate polarization-contrast imaging of unmodified living cells with a resolution significantly exceeding that achievable with conventional instruments. This non-algorithmic, low-phototoxicity imaging technology is a powerful tool both for biological research and for super-resolution imaging of samples that do not allow labeling, such as the interior of silicon chips.
2378-0967
1-10
Rogers, Edward
b92cc8ab-0d91-4b2e-b5c7-8a2f490a36a2
Quraishe, Shmma
31673f5e-736f-4849-ba79-6e078cbd2cb2
Rogers, Katrine S.
499a0078-5e16-4db9-8a98-19825985fb85
Newman, Tracey
322290cb-2e9c-445d-a047-00b1bea39a25
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Zheludev, Nikolai
32fb6af7-97e4-4d11-bca6-805745e40cc6
Rogers, Edward
b92cc8ab-0d91-4b2e-b5c7-8a2f490a36a2
Quraishe, Shmma
31673f5e-736f-4849-ba79-6e078cbd2cb2
Rogers, Katrine S.
499a0078-5e16-4db9-8a98-19825985fb85
Newman, Tracey
322290cb-2e9c-445d-a047-00b1bea39a25
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Zheludev, Nikolai
32fb6af7-97e4-4d11-bca6-805745e40cc6

Rogers, Edward, Quraishe, Shmma, Rogers, Katrine S., Newman, Tracey, Smith, P.J.S. and Zheludev, Nikolai (2020) Far-field unlabelled super-resolution imaging with superoscillatory illumination. APL Photonics, 5, 1-10, [066107]. (doi:10.1063/1.5144918).

Record type: Article

Abstract

Unlabeled super-resolution is the next grand challenge in imaging. Stimulated emission depletion and single-molecule microscopies have revolutionized the life sciences but are still limited by the need for reporters (labels) embedded within the sample. While the Veselago–Pendry “super-lens,” using a negative-index metamaterial, is a promising idea for imaging beyond the diffraction limit, there are substantial technological challenges to its realization. Another route to far-field subwavelength focusing is using optical superoscillations: engineered interference of multiple coherent waves creating an, in principle, arbitrarily small hotspot. Here, we demonstrate microscopy with superoscillatory illumination of the object and describe its underlying principles. We show that far-field images taken with superoscillatory illumination are themselves superoscillatory and, hence, can reveal fine structural details of the object that are lost in conventional far-field imaging. We show that the resolution of a superoscillatory microscope is determined by the size of the hotspot, rather than the bandwidth of the optical instrument. We demonstrate high-frame-rate polarization-contrast imaging of unmodified living cells with a resolution significantly exceeding that achievable with conventional instruments. This non-algorithmic, low-phototoxicity imaging technology is a powerful tool both for biological research and for super-resolution imaging of samples that do not allow labeling, such as the interior of silicon chips.

Text
1.5144918 - Version of Record
Available under License Creative Commons Attribution.
Download (6MB)

More information

Submitted date: 12 January 2010
Accepted/In Press date: 20 May 2020
e-pub ahead of print date: 19 June 2020
Published date: 19 June 2020

Identifiers

Local EPrints ID: 441665
URI: http://eprints.soton.ac.uk/id/eprint/441665
ISSN: 2378-0967
PURE UUID: b5519451-9aeb-4436-a7fb-8261501e8e70
ORCID for Tracey Newman: ORCID iD orcid.org/0000-0002-3727-9258
ORCID for P.J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853
ORCID for Nikolai Zheludev: ORCID iD orcid.org/0000-0002-1013-6636

Catalogue record

Date deposited: 23 Jun 2020 16:54
Last modified: 29 Jul 2020 01:39

Export record

Altmetrics

Contributors

Author: Edward Rogers
Author: Shmma Quraishe
Author: Katrine S. Rogers
Author: Tracey Newman ORCID iD
Author: P.J.S. Smith ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×